• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Exploration of a system for cellular response to the electrophilesexhibiting skin sensitization potential

Research Project

Project/Area Number 23659063
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental pharmacy
Research InstitutionUniversity of Tsukuba

Principal Investigator

KUMAGAI Yoshito  筑波大学, 医学医療系, 教授 (00250100)

Co-Investigator(Kenkyū-buntansha) SHINKAI Yasuhiro  筑波大学, 医学医療系, 助教 (10454240)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords化学物質過敏症 / 3,4-ジヒドロクマリン / アミノ基 / 共有結合
Research Abstract

3,4-dihydrocoumarin (3,4-DHC) is known to be a potent skin sensitizer that has electrophilic property to from a covalent bond with deprotonated amino groups. Incubation of 3,4-DHC in aqueous solution resulted in hydrolysis to form 3-(2-hydroxyphenyl)propanoic acid (HPA) and formation of 3,4-DHC-lysine adduct in the presence of lysine. Also, LC-MS analysis showed that 3,4-DHC modified lysine residues in KLH. By using such a 3,4-DHC-KLH adduct, we prepared the polyclonal antibody against 3,4-DHC.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (10 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] The chemical biology of naphthoquinones and its environmental implications2012

    • Author(s)
      Kumagai Y
    • Journal Title

      Annual Review of Pharmacology and Toxicology

      Volume: 52 Issue: 1 Pages: 221-247

    • DOI

      10.1146/annurev-pharmtox-010611-134517

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Redox cycling of 1,2-naphthoquinone by thioredoxin1 through Cys32 and Cys35 causes inhibition of its catalytic activity and activation of ASK1/p38 signaling.2012

    • Author(s)
      Shinkai Y
    • Journal Title

      Chemical Research in Toxicology

      Volume: 25 Issue: 6 Pages: 1222-1230

    • DOI

      10.1021/tx300069r

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 有機-無機ハイブリッド分子による生体防御系の活性化.2013

    • Author(s)
      藤江智也, 中寛史, 立浪忠志, 山本千夏, 廣岡孝志, 安池修之, 新開泰弘,熊谷嘉人, 内山 真伸, 鍜冶利幸
    • Organizer
      日本薬学会第133 年会
    • Place of Presentation
      横浜
    • Year and Date
      2013-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Activation of Nrf2 caused by tert-butylbenzoquinone, a metabolite of butylated hydroxyanisole, requires electrophilic modification of Keap1 through its reactivethiols2012

    • Author(s)
      Abiko Y
    • Organizer
      The 6th International Congress of Asian Society of Toxicology
    • Place of Presentation
      仙台
    • Year and Date
      2012-07-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 有害金属の毒性に対する防御応答を担う細胞応答システム2012

    • Author(s)
      新開泰弘
    • Organizer
      第39回日本毒性 学会学術年会
    • Place of Presentation
      仙台(招待講演
    • Year and Date
      2012-07-18
    • Related Report
      2012 Final Research Report
  • [Presentation] タンパク質と共有結合する薬毒物の生体反応とそれに対する細胞の防御戦略2011

    • Author(s)
      熊谷嘉人
    • Organizer
      安全性評価研究会セミナー
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2011-12-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 環境中親電子物質によるタンパク質の化学修飾を制御する細胞内システム2011

    • Author(s)
      熊谷嘉人
    • Organizer
      九州大学・生体防御医学研究所 共同利用研究集会
    • Place of Presentation
      福岡(招待講演)
    • Year and Date
      2011-07-22
    • Related Report
      2012 Final Research Report
  • [Presentation] タンパク質と共有結合する薬毒物の生体反応とそれに対する細胞の防御戦略.2011

    • Author(s)
      熊谷嘉人
    • Organizer
      安全性評価研究会セミナー(招待講演)
    • Place of Presentation
      明治製菓ビル(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 環境中親電子物質によるタンパク質の化学修飾を制御する細胞内システム.2011

    • Author(s)
      熊谷嘉人
    • Organizer
      九州大学・生体防御医学研究所 共同利用 研究集会(招待講演)
    • Place of Presentation
      九州大学 (福岡県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 有機-無機ハイブリッド分子による生体防御系の活性化.

    • Author(s)
      藤江智也
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] 有害金属の毒性に対する防御応答を担う細胞応答システム.

    • Author(s)
      新開泰弘
    • Organizer
      第39回日本毒性 学会学術年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Activation of Nrf2 caused by tert-butylbenzoquinone, a metabolite of butylated hydroxyanisole, requires electrophilic modification of Keap1 through its reactivethiols.

    • Author(s)
      Abiko Y
    • Organizer
      The 6th International Congress of Asian Society of Toxicology
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/environmental_medicine/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 環境生物学研究室

    • URL

      http://www.md.tsukuba.ac.jp/environmental_medicine/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/environmental_medicine/index.html

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi