Development of detoxification system of phenolic endocrine disruptersusing immobilized Portulaca PPO

• Project/Area Number: 23659064
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental pharmacy
• Research Institution: Osaka University
• Principal Investigator: HIRATA Kazumasa 大阪大学, 薬学研究科, 教授 (30199062)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 内分泌かく乱物質 / 生体機能利用 / 環境 / 水質汚濁・土壌汚染防止・浄化 / 酵素 / 内分泌撹乱物質 / 内分泌攪乱物質

Research Abstract

To develop a novel detoxification system of phenolic endocrine disrupters in wastewater by using Portulaca’ s polyphenol oxidase (PoPPO), we i) identified PoPPO genes and ii) studied protocols to prepare PoPPO-immobilized materials. i) We obtained 5 PPO homologues from Portulaca’ s root. Tabacco cultured cells transformed with PoPPOs showed metabolic activities of endocrine disrupters. ii) We succeeded to prepare glass beads immobilized with enzymes from Portulaca’ s root, and showed its high stabilities against wide range of pH and temperature.

Research Products

• [Journal Article] Isolation of polyphenol oxidase genes from Portulaca oleracea and evaluation of their ability to metabolize endocrine-disrupting chemicals (2012)
  • Author(s): Kaneda H, Matsui T, Tomiyasu R, Kuroda Y, Higashimoto Y, Oda T, Miyasaka H, Okuhata H, Tanaka S, Harada K, Matsuura H, Nakayama H, Kato K, Hirata K
  • Journal Title: Plant Biotechnol Volume: 29 Pages: 351-357
  • NAID: 10031122159
  • Peer Reviewed
• [Journal Article] Characterization of bisphenol A metabolites produced by Portulaca oleracea cv. using liquid chromatography coupled with tandem mass spectrometry. (2012)
  • Author(s): Watanabe, I., Harada, K., Matsui, T., Miyasaka, H., Okuhata, H., Tanaka, S., Nakayama, H., Kato, K., Bamba, T., Hirata, K.
  • Journal Title: Biosci.Biotechnol. Biochem. Volume: 76 Pages: 1015-1017
  • Peer Reviewed
• [Journal Article] Characterization of Bisphenol A Metabolites Produced by <i>Portulaca oleracea</i> cv. by Liquid Chromatography Coupled with Tandem Mass Spectrometry (2012)
  • Author(s): Watanabe, I.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 5 Pages: 1015-1017
  • DOI: 10.1271/bbb.110967
  • NAID: 10030753179
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Isolation of polyphenol oxidase genes from <i>Portulaca oleracea</i> and evaluation of their ability to metabolize endocrine-disrupting chemicals (2012)
  • Author(s): Kaneda, H.
  • Journal Title: Plant Biotechnology Volume: 29 Issue: 4 Pages: 351-357
  • DOI: 10.5511/plantbiotechnology.12.0423a
  • NAID: 10031122159
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Characterization of bisphenol A metabolites produced by Portulaca oleracea cv. using liquid chromatography coupled with tandem mass spectrometry (2012)
  • Author(s): Watanabe, I., Harada, K., Matsui, T., Miyasaka, H., Okuhata, H., Tanaka, S., Nakayama, H., Kato, K., Bamba, T., Hirata, K.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: in press
  • Peer Reviewed
• [Journal Article] Molecular cloning and partial characterization of a peroxidase gene expressed in the roots of Portulaca oleracea cv., One potentially useful in the remediation of phenolic pollutants (2011)
  • Author(s): Matsui, T., Nomura, Y., Takano, M., Imai, S., Nakayama, H., Miyasaka, H., Okuhara, H., Tanaka, S., Matsuura, H., Harada, K., Bamba, T., Hirata, K., Kato, K
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 75 Pages: 882-890
  • NAID: 10028272506
  • Peer Reviewed
• [Journal Article] Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants (2011)
  • Author(s): Matsui, T., Nomura, Y., Takano, M., Imai, S., Nakayama, H., Miyasaka, H., Okuhara, H., Tanaka, S., Matsuura, H., Harada, K., Bamba, T., Hirata, K., Kato, K.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 5 Pages: 882-890
  • DOI: 10.1271/bbb.100823
  • NAID: 10028272506
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] 園芸植物Portulaca oleracea による内分泌攪乱物質代謝機構の解明 (2011)
  • Author(s): 金田洋和,松井健史,黒田友佳子,東本祐佳,奥 畑博史,田中聡, 松浦秀幸,原田和生,宮坂均 , 加藤晃 , 平田收正
  • Organizer: 第14回環境ホルモン学会
  • Place of Presentation: 東京大学山上会館
• [Presentation] 園芸植物Portulaca oleracea 由来ポリフェノールオキシダーゼの内分泌攪乱物質代謝能の解析 (2011)
  • Author(s): 金田洋和,松井健史,黒田友佳子,東本祐佳,奥 畑博史,田中聡, 松浦秀幸,原田和生,宮坂均 , 加藤晃 , 平田收正
  • Organizer: 第29回日本植物細胞分子生物学会
  • Place of Presentation: 九州大学箱崎キャンパス
• [Presentation] 園芸植物Portulaca oleracea由来ポリフェノールオキシダーゼの内分泌攪乱物質代謝能の解析 (2011)
  • Author(s): 金田洋和，松井健史，黒田友佳子，東本祐佳，奥畑博史，田中聡，松浦秀幸，原田和生，宮坂均，加藤晃，平田收正
  • Organizer: 第29回日本植物細胞分子生物学会
  • Place of Presentation: 九州大学箱崎キャンパス
• [Presentation] 園芸植物Portulaca oleraceaによる内分泌攪乱物質代謝機構の解明 (2011)
  • Author(s): 金田洋和，松井健史，黒田友佳子，東本祐佳，奥畑博史，田中聡，松浦秀幸，原田和生，宮坂均，加藤晃，平田收正
  • Organizer: 第14回環境ホルモン学会
  • Place of Presentation: 東京大学山上会館
• [Book] 環境浄化: 植物による環境ホルモン分解と排水処理への応用 (植物機能のポテンシャルを生かした環境保全・浄化技術 -地球を救う超環境適合・自然調和型システム-) (2011)
  • Author(s): 松浦秀幸, 原田和生, 宮坂均, 平田收正
  • Publisher: シーエムシー出版
• [Book] 環境浄化：植物による環境ホルモン分解と排水処理への応用 （植物機能のポテンシャルを生かした環境保全・浄化技術 -地球を救う超環境適合・自然調和型システム- (2011)
  • Author(s): 松浦秀幸，原田和生，宮坂均，平田收正
  • Publisher: シーエムシー出版