| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Highly pathogenic avian influenza (HPAI) H5N1 virus was characterized by the high pathogenicity and unique organ tropism for infected avian and mammalian. Here we show a new internalized pathway of HPAI H5N1 virus without 2-3 linked sialic acids on the cell surface. We revealed that HPAI H5N1 virus but not low pathogenic avian influenza (LPAI) H5N1 virus could infect in cells treated with neuraminidase for removing sialic acid on the cell surface, and the infected cells generated viral particles with intact infectivity. We then identified the multi-basic sequence (MBS) of HPAI H5N1 as a kind of cell penetrating peptides (CPPs) which is known to have an ability of penetrating cellular membrane. GFP fused with MBS could internalize into several kinds of cells, and specific antibody against MBS prevented the fusion protein from internalizing.
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