Analysis of low temperature tolerance of membrane transporters and development of pan-transporter inhibitors
| Project/Area Number |
23659081
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
TAKANO Mikihisa 広島大学, 医歯薬保健学研究院(薬), 教授 (20211336)
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| Co-Investigator(Renkei-kenkyūsha) |
YUMOTO Ryoko 広島大学, 大学院・医歯薬保健学研究院, 助教 (70379915)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 薬物動態 / 生体膜輸送 / トランスポーター / 低温忍容性 / 汎トランスポーター阻害剤 / 薬学 / 膜小胞 / 輸送阻害剤 / 培養細胞 / GLUT / 生体膜輸送研究 / 赤血球膜小胞 / 腎刷子縁膜小胞 / ENT / MCT |
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| Research Abstract |
Low temperature tolerance of membrane transporters, a characteristic showing transport activity even at ice-cold temperature, was examined in human erythrocytes and cultured cancer cells. A facilitative glucose transporter GLUT1 and a facilitative nucleoside transporter ENT1 showed marked low temperature tolerance in erythrocyte membrane vesicles. A secondary active monocarboxylate transporter MCT1 showed low temperature tolerance in erythrocytes. In addition, GLUT1 in culture cells such as A549 and HepG2 also showed low temperature tolerance.
In order to evaluate the function of these transporters properly, I attempted to develop novel pan-transporter inhibitors (stop and washing solutions for transport study). Among various reagents tested, solutions containing urea plus formaldehyde and 2-mercaptoethanol plus glutaraldehyde were found to be effective pan-transporter inhibitors in both membrane vesicle and culture cell studies.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Characterization of Protamine Uptake by Opossum Kidney Epithelial Cells2013
Author(s)
Nagai, J., Komeda, T., Katagiri, Y., Yumoto, R., Takano, M.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 36
Issue: 12
Pages: 1942-1949
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Endocytic Uptake of FITC-albumin by Human Alveolar Epithelial Cell Line A5492012
Author(s)
Yumoto, R., Suzuka, S., Oda, K., Imaoka, H. and Nagai, J. and Takano, M.
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Journal Title
Drug Metabolism and Pharmacokinetics
Volume: 27
Issue: 3
Pages: 336-343
DOI
NAID
ISSN
1347-4367, 1880-0920
Related Report
Peer Reviewed
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