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Development of novel vaccine system with nanocarrier

Research Project

Project/Area Number 23659082
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Medical pharmacy
Research InstitutionThe University of Tokushima

Principal Investigator

ISHIDA Tatsuhiro  徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (50325271)

Co-Investigator(Kenkyū-buntansha) KIWADA Hiroshi  徳島大学, 大学院・へルスバイオサイエンス研究部, 教授 (50120184)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords薬物動態 / 代謝学 / ワクチン / アジュバント / リポソーム / DDS / PEG / 抗原デリバリー / ポリエチレングリコール / 免疫反応
Research Abstract

We showed that second dose PEGylated liposome is aggressively transported from marginal zone (MZ) into follicle in spleen when they are injected twice into the same rat with short interval1. We revealed that splenic MZ B cells capture and transport second dose liposomes into follicle in a time after second dose injection-dependent manner. The captured and then transported liposomes were finally taken up by follicular dendritic cell. We tried to apply this unique immune response to be a novel adjuvant system which enhances a specific antibody response against antigen encapsulated in second dose liposomes. Pre-immunization with low dose empty liposome, which triggers the transport of antigen-containing second dose liposomes into follicle, induced both anti-OVA IgM and anti-OVA IgG productions as OVA-containing liposomes, not free OVA, was intravenously injected as a second dose. The produced anti-OVA IgG contained IgG1, IgG2a and IgG2b subclasses. In addition, the high level of anti-OVA IgG lasted over 12 weeks after the immunization. These suggest that pre-stimulation with empty PEGylated liposomes could enhance the specific antibody response against antigen encapsulated in second dose liposomes. We conclude that our active transport methodology can be useful for a novel and alternative vaccine strategy to potentiate specific antibody response.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2013 2012 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (10 results)

  • [Journal Article] Transport of PEGylated liposomes from the splenic marginal zone to the follicle in the induction phase of the accelerated blood clearance phenomenon.2013

    • Author(s)
      Shimizu T
    • Journal Title

      Immunobiol.

      Volume: 218 Issue: 5 Pages: 725-732

    • DOI

      10.1016/j.imbio.2012.08.274

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] PEG修飾リポソームを用いた濾胞への高原送達による抗体誘導効果2013

    • Author(s)
      石田竜弘
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2013-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] PEG修飾リポソームを用いた濾胞への高原送達による抗体誘導効果2013

    • Author(s)
      清水太郎
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Activation of splenic marginal zone B cell by PEGylated liposome with lower dose: triggering transport of antigen-containing second dose PEGylated liposome from marginal zone to follicle2012

    • Author(s)
      Ishida, T
    • Organizer
      39th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Centre des Congress de Quebec(カナダケベック)
    • Year and Date
      2012-07-16
    • Related Report
      2012 Final Research Report
  • [Presentation] PEG 修飾リポソームと脾臓辺縁帯 B 細胞との相互作用に関する検討2012

    • Author(s)
      石田 竜弘
    • Organizer
      日本薬学会第132 年会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 腫瘍内微小環境の能動的制御に基づくsiRNAデリバリー技術の開発とがん治療への展開2012

    • Author(s)
      石田 竜弘
    • Organizer
      日本薬学会第132 年会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-03-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Activation of splenic marginal zone B cell by PEGylated liposome with lower dose: triggering transport of antigen-containing second dose PEGylated liposome from marginal zone to follicle.2012

    • Author(s)
      Shimizu, T.
    • Organizer
      39th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Centre des congres de Quebec (カナダ)
    • Related Report
      2012 Annual Research Report
  • [Presentation] PEG修飾リポソームと脾臓辺縁帯B 細胞との相互作用に関する検討2012

    • Author(s)
      清水太郎
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] 腫瘍内微小環境の能動的制御に基づくsiRNAデリバリー技術の開発とがん治療への展開2012

    • Author(s)
      石田竜弘
    • Organizer
      日本薬学会第132年会(招待講演)
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Anti-PEG IgM分泌誘導においてPEG修飾剤末端構造が与える影響に関する検討2011

    • Author(s)
      石田 竜弘
    • Organizer
      第50回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      サンポートホール高松(高松市)
    • Year and Date
      2011-11-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Anti-PEG IgM分泌誘導においてPEG修飾剤末端構造が与える影響に関する検討2011

    • Author(s)
      藤田理沙子
    • Organizer
      第50回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      サンポート高松(香川県)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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