| Project/Area Number |
23659085
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
YUASA Hiroaki 名古屋市立大学, 大学院・薬学研究科, 教授 (20191471)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Katsuhisa 名古屋市立大学, 大学院・薬学研究科, 准教授 (50315892)
OHTA Kinya 名古屋市立大学, 大学院・薬学研究科, 助教 (90448704)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | トランスポーター / 樹状細胞 / 小胞体 / 免疫 / 抗アレルギー薬 / 創薬標的 |
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| Research Abstract |
Functional characteristics of human vesicular organic anion transporter 1 (VOAT1) were examined by assessing the cellular uptake (accumulation in the endoplasmic reticulum) of 5-aminofluorescein (5-AF) as a fluorescent probe substrate, based on the observation of fluorescent microscopic images. 5-AF uptake was found to be specifically inhibited by several anti-allergic drugs, anti-inflammatory drugs, and endogenous fatty acids, including their oxidative products. These findings suggest that VOAT1 might be involved in the transport of lipid mediators and, thereby, play a role in inflammatoric and immunologic processes. It might also be involved in the pharmacologic action of anti-inflammatory drugs. Thus, we could obtain valuable information about the functional characteristics of VOAT1, although it needs to be further validated by quantitative assessments.
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