Induction of spermatogenic cells from human multipotent stem cell by microinjection into the mouse testis
| Project/Area Number |
23659090
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO Chizuru 千葉大学, 大学院・医学研究院, 講師 (80347054)
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| Co-Investigator(Renkei-kenkyūsha) |
MAEKAWA Mamiko 千葉大学, 大学院・医学研究院, 助教 (20181571)
YAMATOYA Kenji 千葉大学, 大学院・医学研究院, 特任研究員 (80447309)
KAMIMURA Kyoko 千葉大学, 大学院・医学研究院, 技術専門職員 (20422264)
MUTOH Tohru 千葉大学, 大学院・医学研究院, 技術専門職員 (30422265)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | Muse細胞 / 精巣内移植 / 精子幹細胞 / 精子形成 / 精子 / 精巣移植 / Muse 細胞 |
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| Research Abstract |
EGFP-tagged Muse cells from human mesenchymal cells were prepared by established method. Thus prepared EGFP-Muse cells were microinjected into the testes (testicular seminiferous tubules) of W/Wv mutant male mice lacking spermatogenic stem cells, and we found the following results. Enough amount of live Muse cells were obtained even after long time journey for several hours. Muse cells prepared caused cell-cell aggregation when they were placed in the conditioned medium containing albumin for microinjection, but the microinjection efficiency rate increased when they were microinjected using medium lacking albumin. However, 3 month later, EGFP-Muse cell signal was not recognized at all in the injected testes, indicating that Muse cells in the transplanted medium in this study could not develop to the spermatogenic cells.
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Report
(3 results)
Research Products
(91 results)
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[Journal Article] Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage2011
Author(s)
Sato B, Katagiri YU, Miyado K, Okino N, Ito M, Akutsu H, Okita H, Umezawa A, Fujimoto J, Toshimori K, Kiyokawa N
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Journal Title
BMC Dev Biol
Volume: 11
Issue: 1
Pages: 22-22
DOI
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Peer Reviewed
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[Presentation] Tysnd1 deficiency interferes with mouse peroxisomal functions2011
Author(s)
Mizuno Y, Ninomiya Y, Iseki M, Iwasa H, Akita M, Tsukui T, Ito C, Toshimori K,Shimozawa N, Nishimukai M, Hara H, MaebaR, Okazaki T, Horai Y, Watanabe M, Motegi H, Wakana S, Noda T, Kurochkin Igor V, Mizuno Y, Schoenbach C, Okazaki Y.
Organizer
第34回日本分子生物学会年会
Place of Presentation
パシフィコ横浜(神奈川県)(口演)
Year and Date
2011-12-15
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[Book] 卵子学2011
Author(s)
年森清隆、伊藤千鶴
Total Pages
1210
Publisher
京都大学学術出版
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