Development of a long-term culture system for functional Sertoli cells.
| Project/Area Number |
23659095
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyoto University |
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Principal Investigator |
SHINOHARA Takashi 京都大学, 医学(系)研究科(研究院), 教授 (30322770)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 精子形成 / セルトリ細胞 / 精子幹細胞 / 増殖 / T antigen |
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| Research Abstract |
It has not been possible to culture functional Sertoli cells for long-term. We have developed a new system for culturing mouse Sertoli cells that provide microenvironment for spermatogonial stem cells. In this culture system, Sertoli cells could maintain spermatogonial stem cell activity for more than five months when they were supplemented with epidermal growth factor and fibroblast growth factor 2. Spermatogonial stem cells migrated under the Sertoli cell feeder layer and formed cobblestone colonies. Cultured spermatogonial stem cells could produce functional sperm upon transplantation into the seminiferous tubules of infertile mouse testes. Because cobblestone colony formation was suppressed by inhibiting CXCR4 or RET, these molecules may be involved in migration of spermatogonial stem cells to germline niche. Taken together, our novel culture system will provide a new system for functional assay of spermatogonial stem cells.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Reconstitution of mouse spermatogonial stem cell niches in culture2012
Author(s)
Kanatsu-Shinohara, M., Inoue, K., Takashima, S.,Takehashi, M.,Ogonuki, N., Morimoto, H., Nagasawa, T., Ogura, A. and Shinohara, T
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Journal Title
Cell Stem Cell
Volume: 11(4)
Issue: 4
Pages: 567-578
DOI
Related Report
Peer Reviewed
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[Journal Article] In vitro transformation of mouse testis cells by oncogene transfection2012
Author(s)
Morimoto, H., Lee, J., Tanaka, T., Ishii, K., Toyokuni, S., Kanatsu-Shinohara, M., Shinohara, T.
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Journal Title
Biology of Reproduction
Volume: 86
Related Report
Peer Reviewed
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[Journal Article] Rac mediates spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier2011
Author(s)
Takashima, S., Kanatsu-Shinohara,M. Tanaka,T., Takehashi, M.,Morimoto, H.,Shinohara, T.
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Journal Title
Cell Stem Cell
Volume: 9
Pages: 463-475
Related Report
Peer Reviewed
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