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Development of a long-term culture system for functional Sertoli cells.

Research Project

Project/Area Number 23659095
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyoto University

Principal Investigator

SHINOHARA Takashi  京都大学, 医学(系)研究科(研究院), 教授 (30322770)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords精子形成 / セルトリ細胞 / 精子幹細胞 / 増殖 / T antigen
Research Abstract

It has not been possible to culture functional Sertoli cells for long-term. We have developed a new system for culturing mouse Sertoli cells that provide microenvironment for spermatogonial stem cells. In this culture system, Sertoli cells could maintain spermatogonial stem cell activity for more than five months when they were supplemented with epidermal growth factor and fibroblast growth factor 2. Spermatogonial stem cells migrated under the Sertoli cell feeder layer and formed cobblestone colonies. Cultured spermatogonial stem cells could produce functional sperm upon transplantation into the seminiferous tubules of infertile mouse testes. Because cobblestone colony formation was suppressed by inhibiting CXCR4 or RET, these molecules may be involved in migration of spermatogonial stem cells to germline niche. Taken together, our novel culture system will provide a new system for functional assay of spermatogonial stem cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2014 2013 2012 2011 Other

All Journal Article (23 results) (of which Peer Reviewed: 9 results) Presentation (2 results) Remarks (3 results)

  • [Journal Article] Cell-cycle-dependent colonization of mouse spermatogonial stem cells after transplantation into seminiferous tubules2014

    • Author(s)
      Ishii K., Kanatsu-Shinohara M., Shinohara T
    • Journal Title

      J. Reprod. Dev

      Volume: 60(1) Pages: 37-46

    • NAID

      130003381858

    • Related Report
      2013 Final Research Report
  • [Journal Article] Cell-cycle-dependent Colonization of Mouse Spermatogonial Stem Cells After Transplantation into Seminiferous Tubules.2014

    • Author(s)
      Ishii K, Kanatsu-Shinohara M, Shinohara T.
    • Journal Title

      J Reprod Dev.

      Volume: 60 Pages: 37-46

    • NAID

      130003381858

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enrichment of mouse spermatogonial stem cells based on aldehyde dehydrogenase activity2013

    • Author(s)
      Kanatsu-Shionohara M., Mori Y., Shinohara T
    • Journal Title

      Biol. Reprod

      Volume: 89(6) Pages: 140-140

    • Related Report
      2013 Final Research Report
  • [Journal Article] Spermatogonial stem cell self-renewal and development2013

    • Author(s)
      Kanatsu-Shinohara M., Shinohara T
    • Journal Title

      Annu. Rev. of Cell and Developmental Biology

      Volume: 29 Pages: 163-187

    • Related Report
      2013 Final Research Report
  • [Journal Article] Regulation of pluripotency in male germline stem cells by Dmrt12013

    • Author(s)
      Takashima S., Hirose M., Ogonuki N., Ebisuya M., Inoue K., Kanatsu-Shinohara M., Tanaka T., Nishida E., Ogura A., Shinohara T
    • Journal Title

      Genes Dev

      Volume: 27(18) Pages: 1949-1958

    • Related Report
      2013 Final Research Report
  • [Journal Article] ROS are required for spermatogonial stem cell self-renewal2013

    • Author(s)
      Morimoto H., Iwata K., Ogonuki N., Inoue K., Ogura A., Kanatsu-Shinohara M., Morimoto T., Yabe-Nishimura C., Shinohara T
    • Journal Title

      Cell Stem Cell

      Volume: 12(6) Pages: 774-786

    • Related Report
      2013 Final Research Report
  • [Journal Article] Enrichment of mouse spermatogonial stem cells based on aldehyde dehydrogenase activity.2013

    • Author(s)
      Kanatsu-Shinohara M, Mori Y, Shinohara T.
    • Journal Title

      Biol Reprod.

      Volume: 89 Issue: 6 Pages: 140-140

    • DOI

      10.1095/biolreprod.113.114629

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spermatogonial stem cell self-renewal and development.2013

    • Author(s)
      Kanatsu-Shinohara M, Shinohara T.
    • Journal Title

      Annu Rev Cell Dev Biol.

      Volume: 29 Issue: 1 Pages: 163-87

    • DOI

      10.1146/annurev-cellbio-101512-122353

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of pluripotency in male germline stem cells by Dmrt1.2013

    • Author(s)
      Takashima S, Hirose M, Ogonuki N, Ebisuya M, Inoue K, Kanatsu-Shinohara M, Tanaka T, Nishida E, Ogura A, Shinohara T.
    • Journal Title

      Genes Dev.

      Volume: 27 Issue: 18 Pages: 1949-58

    • DOI

      10.1101/gad.220194.113

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ROS are required for mouse spermatogonial stem cell self-renewal.2013

    • Author(s)
      Morimoto H, Iwata K, Ogonuki N, Inoue K, Atsuo O, Kanatsu-Shinohara M, Morimoto T, Yabe-Nishimura C, Shinohara T.
    • Journal Title

      Cell Stem Cell.

      Volume: 12 Issue: 6 Pages: 774-86

    • DOI

      10.1016/j.stem.2013.04.001

    • NAID

      120005285490

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enrichment of mouse spermatogaonial stem cells by melanoma cell adhesion molecule expression2012

    • Author(s)
      Kanatsu-Shinohara M., Morimoto H., Shinohara T
    • Journal Title

      Biol. Reprod

      Volume: 87(6) Pages: 139-139

    • Related Report
      2013 Final Research Report
  • [Journal Article] Reconstitution of mouse spermatogonial stem cell niches in culture2012

    • Author(s)
      Kanatsu-Shinohara M., Inoue K., Takashima S., Takehashi M., Ogonuki N., Morimoto H., Nagasawa T., Ogura A., Shinohara T
    • Journal Title

      Cell Stem Cell

      Volume: 11 Pages: 567-578

    • Related Report
      2013 Final Research Report
  • [Journal Article] FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAPK2K1 activation2012

    • Author(s)
      Ishii K., Kanatsu-Shinohara M., Toyokuni S., Shinohara T
    • Journal Title

      Development

      Volume: 139(10) Pages: 1734-1743

    • Related Report
      2013 Final Research Report
  • [Journal Article] Hybridization of testis-derived stem cells with somatic cells and embryonic stem cells in mice2012

    • Author(s)
      Takehashi M., Tada M., Kanatsu-Shinohara M., Morimoto H., Kazuki Y., Oshimura M., Tada T., Shinohara T
    • Journal Title

      Biol. Reprod

      Volume: 86(6) Pages: 178-178

    • Related Report
      2013 Final Research Report
  • [Journal Article] In vitro transformation of mouse testis cells by oncogene transfection2012

    • Author(s)
      Morimoto H., Lee J., Tanaka T., Ishii K., Toyokuni S., Kanatsu-Shinohara M., Shinohara T
    • Journal Title

      Biol. Reprod

      Volume: 86(5)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Reconstitution of mouse spermatogonial stem cell niches in culture2012

    • Author(s)
      Kanatsu-Shinohara, M., Inoue, K., Takashima, S.,Takehashi, M.,Ogonuki, N., Morimoto, H., Nagasawa, T., Ogura, A. and Shinohara, T
    • Journal Title

      Cell Stem Cell

      Volume: 11(4) Issue: 4 Pages: 567-578

    • DOI

      10.1016/j.stem.2012.06.011

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vitro transformation of mouse testis cells by oncogene transfection2012

    • Author(s)
      Morimoto, H., Lee, J., Tanaka, T., Ishii, K., Toyokuni, S., Kanatsu-Shinohara, M., Shinohara, T.
    • Journal Title

      Biology of Reproduction

      Volume: 86

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rac mediates mouse spermatogonial stem cell homing to germline nishes by regulating transmigration through the blood-testis barrier2011

    • Author(s)
      Takashima S., Kanatsu-Shinohara M., Tanaka T., Takehashi M., Morimoto H., Shinohara T
    • Journal Title

      Cell Stem Cell

      Volume: 9(5) Pages: 463-475

    • Related Report
      2013 Final Research Report
  • [Journal Article] Dynamic changes in EPCAM expression during spermatogonial stem cell differentiation in the mouse testis2011

    • Author(s)
      Kanatsu-Shinohara M., Takashima S., Ishii K., Shinohara T
    • Journal Title

      PLoS One

      Volume: 6(8)

    • NAID

      120003405532

    • Related Report
      2013 Final Research Report
  • [Journal Article] Homologous recombination in rat germline stem cells2011

    • Author(s)
      Kanatsu-Shinohara M., Kato-Itoh M., Ikawa M., Takehashi M., Sanbo M., Morioka Y., Tanaka T., Morimoto H., Hirabayashi M., Shinohara T
    • Journal Title

      Biol. Reprod

      Volume: 85(1) Pages: 208-217

    • Related Report
      2013 Final Research Report
  • [Journal Article] Unstable side population phenotype of mouse spermatogonial stem cells in vitro2011

    • Author(s)
      Shinohara T., Ishii K., Kanatsu-Shinohara M
    • Journal Title

      J. Reprod. Dev

      Volume: 57(2) Pages: 288-295

    • NAID

      10028275625

    • Related Report
      2013 Final Research Report
  • [Journal Article] Dynamic changes in EPCAM expression during spermatogonial stem cell differentiation in the mouse testis2011

    • Author(s)
      Kanatsu-Shinohara M., Takashima S., Shinohara T.
    • Journal Title

      PLoS One

      Volume: 6

    • NAID

      120003405532

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rac mediates spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier2011

    • Author(s)
      Takashima, S., Kanatsu-Shinohara,M. Tanaka,T., Takehashi, M.,Morimoto, H.,Shinohara, T.
    • Journal Title

      Cell Stem Cell

      Volume: 9 Pages: 463-475

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Derivation of stem cell lines from the male germline2012

    • Author(s)
      Takashi Shinohara
    • Organizer
      International Workshop: Radiation Effects on Mutation in Somatic and Germline Stem Cell(招待講演)
    • Place of Presentation
      放影研(広島県)
    • Related Report
      2011 Research-status Report
  • [Presentation]

    • Organizer
      Brinster symposium
    • Related Report
      2013 Final Research Report
  • [Remarks] 新聞での報道 2011年11月5日 京都新聞朝刊 27面京大グループ 精子幹細胞の増殖に働くタンパク質特定 移植治療への応用に期待

    • Related Report
      2013 Final Research Report
  • [Remarks] 新聞での報道 2012年10月5日 京都新聞朝刊 24面精子幹細胞 増殖環境を再現-京大など, 試験管内に 移植治療に応用期待

    • Related Report
      2013 Final Research Report
  • [Remarks] 新聞での報道 2012年10月5日 産經新聞朝刊 23面精巣内定着率向上 タンパク質特定 京大グループ

    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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