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Detection and functional analysis of cardiac stem cells

Research Project

Project/Area Number 23659096
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including Histology/Embryology)
Research InstitutionHiroshima University

Principal Investigator

TAKIHARA Yoshihiro  広島大学, 原爆放射線医科学研究所, 教授 (60226967)

Co-Investigator(Kenkyū-buntansha) YASUNAGA Shin'ichiro  広島大学, 原爆放射線医科学研究所, 准教授 (50336111)
SHIRAI Manabu  独立行政法人国立循環器病研究センター, 分子生物部, 室長 (70294121)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords細胞分化 / 組織形成 / 心臓幹細胞 / 再生医学 / ポリコーム複合体 / Geminin
Research Abstract

In this study we further analyzed a molecular role for the Polycomb-rgoup (PcG) complex 1 including Rae28/Phc1 and attempted to identify cardiac stem cells focusing on the expression level of Geminin. PcG complex 1 regulates protein stability through the ubiquitin-proteasome system as well as transcription through the chromatin regulation. We here identified a novel PcG complex with Pcgf5 in the heart and Gemininhighcellular subpopulations in the heart. Since Gemininhighcells reside in the cardiac atrium, in which there previously shown was the presence of cardiac stem cells, these are presumed to be candidates for cardiac stem cells. On the other hand, expression of Geminin is high in S-phase cells, suggesting an intriguing possibility that Gemininhigh cells are proliferating cells in the adult heart.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (21 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (11 results) Remarks (2 results)

  • [Journal Article] Aurora-A controls pre-replicative complex formation and DNA replication by promoting the stabilization of geminin in mitosis2013

    • Author(s)
      Tsunematsu, T., Takihara, Y., Ishimura, N., Pagano, M., Takata, T., Kudo, Y.
    • Journal Title

      Nature Commun.

      Volume: 4 Pages: 1885-1885

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hoxa9 transduction induces mouse hematopoietic stem and progenitor cell activity through direct down-regulation of Geminin.2013

    • Author(s)
      Ohno, Y., Yasunaga, S., Janmohamed, S., Ohtubo, M., Mihara, K., Iscove, N., Takihara, Y.
    • Journal Title

      PLoS ONE

      Volume: 8(1) Issue: 1 Pages: e53161-e53161

    • DOI

      10.1371/journal.pone.0053161

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Scmh1 has E3 ubiquitin ligase activity for Geminin and histone H2A and regulates Geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb42013

    • Author(s)
      Yasunaga, S., Ohtsubo, M., Ohno, Y., Tanaka-Okamoto, M., Ishizaki, H., Shirai, M., Mihara, K., Brock, W. H., Miyoshi, J., Takihara, Y.
    • Journal Title

      Mol. Cell. Biol

      Volume: 33 Pages: 644-660

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] MK3 controls Polycomb-target gene expression via negative feedback on ERK.2013

    • Author(s)
      Prickaerts, P.
    • Journal Title

      Epigenetics Chromatin

      Volume: 5 Issue: 1 Pages: 12-12

    • DOI

      10.1186/1756-8935-5-12

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Scmh1 has E3 ubiquitin ligase activity for Geminin and histone H2A and regulates Geminin stability directly or indirectly via transcription repression of Hoxa9 and Hoxb42013

    • Author(s)
      Yasunaga, S., Ohtsubo, M., Ohno, Y., Saeki, K., Kurogi. T., Tanaka-Okamoto, M., Ishizaki, H., Shirai, M., Mihara, K., Brock, H. W., Miyoshi, J. & Takihara Y
    • Journal Title

      Mol. Cell. Biol.

      Volume: 33(4) Issue: 4 Pages: 644-660

    • DOI

      10.1128/mcb.00974-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 幹細胞における細胞周期制御特集:がん幹細胞研究の新たな展開2012

    • Author(s)
      瀧原義宏、安永晋一郎、大野芳典、大坪素秋
    • Journal Title

      血液フロンティア(医薬ジャーナル社)

      Volume: 22 Pages: 33-43

    • Related Report
      2012 Final Research Report
  • [Journal Article] Role for Geminin in sustaining the activity of hematopoietic stem cells.2011

    • Author(s)
      Takihara, Y.
    • Journal Title

      Cell Cycle

      Volume: 10 Pages: 561-562

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 造血幹細胞の守護神Geminin2011

    • Author(s)
      瀧原義宏
    • Journal Title

      原医研ニュース

      Volume: 2 Pages: 6-7

    • Related Report
      2012 Final Research Report
  • [Presentation] Novel molecular regulatory network governing Geminin maintains hematopoietic homeostasis.2012

    • Author(s)
      Takihara, Y., Yasunaga, S., Ohno, Y., Saeki, K., Nakashima, Y., Ohtsubo, M.
    • Organizer
      41st annual scientific meeting of the international society for hematology and stem cells
    • Place of Presentation
      Amsterdam, Netherlands
    • Related Report
      2012 Final Research Report
  • [Presentation] Scmh1, a member of Polycomb-complex 1, maintains hematopoietic homeostasis through the regulation of Geminin protein stability2012

    • Author(s)
      Takihara, Y., Ohno, Y.,Yasunaga, S., Saeki, K., Kurogi, T., Nakashima, Y., Ohtsubo, M.
    • Organizer
      International symposium: Genetic and Epigenetic Control of Cell Fate
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Final Research Report
  • [Presentation] Functional analysis of Pcgf5 (Polycomb Ring Finger 5) gene during Cardiogenesis.2012

    • Author(s)
      Shirai, M., Takihara, Y., Morisaki, T
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Scmh1 is required for maintaining hematopoietic homeostasis through the molecular network governing Geminin expression.2012

    • Author(s)
      Ohno, Y., Yasunaga, S., Saeki, K., Ohtsubo, M., Kurogi, T., Nakashima, Y., Takihara, Y.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular role for Geminin in E2F-mediated transcriptional regulation2012

    • Author(s)
      Saeki, K., Ohno, Y., Yasunaga, S., Ohtsubo, M., Kurogi, T., Nakashima, Y., Takihara, Y.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] 心臓発生における、ポリコーム遺伝子群の発現及び機能解析2012

    • Author(s)
      白井 学、瀧原義宏、森崎隆幸
    • Organizer
      日本心臓血管発生研究会
    • Place of Presentation
      熱海
    • Related Report
      2012 Final Research Report
  • [Presentation] ポリコーム複合体1と Hoxb4による Geminin 制御と造血幹細胞活性2012

    • Author(s)
      瀧原義宏
    • Organizer
      特定領域研究「細胞増殖制御」終了シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] ポリコーム遺伝子群の心臓形態形成に対する発現・機能解析2012

    • Author(s)
      白井 学
    • Organizer
      心臓血管発生研究会
    • Place of Presentation
      郡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] ポリコーム遺伝子群の心臓形態形成に対する発現・機能解析2011

    • Author(s)
      白井 学、瀧原義宏、森崎隆幸
    • Organizer
      心臓血管発生研究会
    • Place of Presentation
      郡山
    • Related Report
      2012 Final Research Report
  • [Presentation] Distribution of Polycomb-group proteins in the developing heart2011

    • Author(s)
      Shirai, M., Takihara, Y., Morisaki, T
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] Distribution of Polycomb-group proteins in the developing heart

    • Author(s)
      Shirai, M.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/dscb/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/dscb/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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