Development of a mouse model for live-imaging of hepatic epithelial patterning in mouse embryonic liver using a HAC carrying BACs
| Project/Area Number |
23659097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kumamoto University |
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Principal Investigator |
YOKOUCHI Yuji 熊本大学, 生命資源研究支援センター, 特任教授 (60252227)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMURA Ken-ichi 熊本大学, 生命資源研究・支援センター, シニア教授 (90115197)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 細胞分化 / 組織形成 / 肝臓 / イメージング / GFP / RFP / 上皮 / BAC / HAC / 組織発生 / 細胞間相互作用 / 血管 / 肝上皮 / 自己組織化 / 蛍光タンパク / 解剖学 / 組織学 / 発生学 / 再生医学 / 三次元 |
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| Research Abstract |
Endodermal organs have tree-like structures of endodermal epithelia which are essential for performing their functions. The structures are autonomously organized by cell-to-cell interactions among different cell populations. As their final tree-like pattern of the endodermal epithelial tubes are fractal, the patterns appear to be formed based on the some mathematical rules. However, the rules have been unknown because we were not able to perform real-time observation of the behaviors of the cells involved. In order to perform simultaneous real-time imaging for different two cell populations in histogenesis of the embryonic organs using Foxa2-nEGFP-CreERT2, WT1-EGFPCre, and Tek-Cre, we generated Ai-3tTom, a new ROSA26 reporter line. This mouse line can expresses myristyl tandem Tomato in a Cre-dependent manner from ROSA26 locus, which emits very strong red fluorescence. Therefore, we are able to perform a real-time live imaging of the cells using this mouse line.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] A Secreted BMP Antagonist, Cerl, Fine Tunes the Spatial Organization of the Ureteric Bud Tree during Mouse Kidney Development2011
Author(s)
Chi, L., Saarela, U., Railo, A., Prunskaite-Hyyrylainen, R., Skovorodkin, I., Anthony, S., Katsu, K., Liu, Y., Shan, J., Salgueiro, AM, Belo, JA, Davies, J., Yokouchi, Y., Vainio, SJ
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Journal Title
PLOS ONE
Volume: 6
Issue: 11
Pages: e27676-e27676
DOI
Related Report
Peer Reviewed
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