Development of a mouse model for live-imaging of hepatic epithelial patterning in mouse embryonic liver using a HAC carrying BACs

• Project/Area Number: 23659097
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kumamoto University
• Principal Investigator: YOKOUCHI Yuji 熊本大学, 生命資源研究支援センター, 特任教授 (60252227)
• Co-Investigator(Renkei-kenkyūsha): YAMAMURA Ken-ichi 熊本大学, 生命資源研究・支援センター, シニア教授 (90115197)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 細胞分化 / 組織形成 / 肝臓 / イメージング / GFP / RFP / 上皮 / BAC / HAC / 組織発生 / 細胞間相互作用 / 血管 / 肝上皮 / 自己組織化 / 蛍光タンパク / 解剖学 / 組織学 / 発生学 / 再生医学 / 三次元

Research Abstract

Endodermal organs have tree-like structures of endodermal epithelia which are essential for performing their functions. The structures are autonomously organized by cell-to-cell interactions among different cell populations. As their final tree-like pattern of the endodermal epithelial tubes are fractal, the patterns appear to be formed based on the some mathematical rules. However, the rules have been unknown because we were not able to perform real-time observation of the behaviors of the cells involved. In order to perform simultaneous real-time imaging for different two cell populations in histogenesis of the embryonic organs using Foxa2-nEGFP-CreERT2, WT1-EGFPCre, and Tek-Cre, we generated Ai-3tTom, a new ROSA26 reporter line. This mouse line can expresses myristyl tandem Tomato in a Cre-dependent manner from ROSA26 locus, which emits very strong red fluorescence. Therefore, we are able to perform a real-time live imaging of the cells using this mouse line.

Research Products

• [Journal Article] Multi-modal effects of BMP signaling on Nodal expression in the lateral plate mesoderm during left-right axis formation in the chick embryo (2013)
  • Author(s): Kenjiro Katsu, Norifumi Tatsumi, Daisuke Niki, Ken-ichi Yamamura, Yuji Yokouchi
  • Journal Title: Developmental Biology Volume: 374 Pages: 71-84
  • Peer Reviewed
• [Journal Article] BMP inhibition by DAN in Hensen's node is a critical step for the establishment of left-right asymmetry in the chick embryo (2012)
  • Author(s): Kenjiro Katsu, Daisuke Tokumori, Norifumi Tatsumi, Atsushi Suzuki, Yuji Yokouchi
  • Journal Title: Developmental Biology Volume: 363 Issue: 1 Pages: 15-26
  • DOI: 10.1016/j.ydbio.2011.12.015
  • Peer Reviewed
• [Journal Article] A Secreted BMP Antagonist, Cerl, Fine Tunes the Spatial Organization of the Ureteric Bud Tree during Mouse Kidney Development (2011)
  • Author(s): Chi, L., Saarela, U., Railo, A., Prunskaite-Hyyrylainen, R., Skovorodkin, I., Anthony, S., Katsu, K., Liu, Y., Shan, J., Salgueiro, AM, Belo, JA, Davies, J., Yokouchi, Y., Vainio, SJ
  • Journal Title: PLOS ONE Volume: 6 Issue: 11 Pages: e27676-e27676
  • DOI: 10.1371/journal.pone.0027676
  • Peer Reviewed
• [Presentation] Functional analysis of the TGF-beta pathway in hepatoblasts during mouse hepatic histogenesis. マウス肝組織形成時の肝芽細胞におけるTGF-beta 経路の機能解析 (2014)
  • Author(s): Yuji Yokouchi, Daiki Yoshii, Hiroki Takeda, Hiroko Suda, Yukihiro Inomata, and Ken-ichi Yamamura
  • Organizer: 47th Annual Meeting of the Japanese Society of Developmental Biologists. Co-sponsor: Asia-Pacific Developmental Biology Network
  • Place of Presentation: Nagoya, WINC AICHI
• [Presentation] Functional Analysis of TGF-beta pathway in hepatoblasts during mouse hepatic histogenesis (2014)
  • Author(s): Yuji Yokouchi, Naoki Takeda, Hiroshi Sasaki, and Ken-ich Yamamura
  • Organizer: 47th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: 名古屋
• [Presentation] 脾臓形成を制御する遺伝子ネットワークの同定とその作用機序 (2012)
  • Author(s): ○勝 賢二郎、辰巳徳史、仁木大輔、横内裕二
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 甲府市
• [Remarks] 横内裕二-研究内容｜熊本大学生命資源研究支援センター 疾患モデル分野
  • URL: http://irda-genetics.kuma-u.jp/research/r02.html
• [Remarks] 横内裕二-研究業績｜熊本大学生命資源研究支援センター 疾患モデル分野
  • URL: http://irda-genetics.kuma-u.jp/publication/p03.html
• [Remarks] 熊本大学 生命資源研究支援センター 疾患モデル分野 研究内容 横内裕二
  • URL: http://irda-genetics.kuma-u.jp/research/r02.html
• [Remarks] 熊本大学 生命資源研究支援センター 疾患モデル分野 研究業績 横内裕二
  • URL: http://irda-genetics.kuma-u.jp/publication/p03.html
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/pattern_formation/