Analysis of non-coding RNA expression in stem and progenitor cells in mouse testes

• Project/Area Number: 23659099
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Yokohama City University
• Principal Investigator: OHBO Kazuyuki 横浜市立大学, 医学研究科, 教授 (70250751)
• Co-Investigator(Renkei-kenkyūsha): MATSUMOTO Naomichi 横浜市立大学, 医学研究科, 教授 (80325638)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 幹細胞 / 精巣 / Non-coding RNA / 細胞分化 / ゲノム修飾 / 生殖細胞 / 前駆細胞 / 小分子RNA

Research Abstract

In mice, we found that the significant progression of heterochromatin formation occurred at the transition from stem to progenitor cells in adult male germ cells. In addition, the gene expression pattern was also significantly changed at the transition. Since it has been reported that non-coding RNA plays an important role in heterochromatin formation, resulting in changing of gene expression profiles, we analyzed and compared the expression profiles of miRNA and long non-coding RNA between stem cells and progenitor cells in adult male germ cells. We found several miRNA and long non-coding RNA that were specifically expressed in each of the cell populations.

Research Products

• [Journal Article] Serum level of HE4 is closely associated with pulmonary adenocarcinoma progression (2012)
  • Author(s): SI. Yamashita, K. Tokuishi, T. Moroga, S. Yamamoto, K. Ohbo, S. Miyahara, Y. Yoshida, J. Yanagisawa, D. Hamatake, M. Hiratsuka, Y. Yoshinaga, T. Shiraishi, A. Iwasaki and K. Kawahara
  • Journal Title: Tumour Biol Volume: 33 Issue: 6 Pages: 2365-2370
  • DOI: 10.1007/s13277-012-0499-8
  • Peer Reviewed
• [Journal Article] Splice variant HE4-V3 expression is associated with favorable prognosis in pulmonary adenocarcinoma (2012)
  • Author(s): K. Tokuishi, SI. Yamashita, K. Ohbo and K. Kawahara
  • Journal Title: Tumour Biol Volume: 33 Issue: 1 Pages: 103-109
  • DOI: 10.1007/s13277-011-0252-8
  • Peer Reviewed
• [Journal Article] Serum level of HE4 is closely associated with pulmonary adenocarcinoma progression. (2012)
  • Author(s): Yamashita S, Tokuishi K, Moroga T, Yamamoto S, Ohbo K, Miyahara S, Yoshida Y, Yanagisawa J, Hamatake D, Hiratsuka M, Yoshinaga Y, Shiraishi T, Iwasaki A, Kawahara K.
  • Journal Title: Tumour Biol Volume: 33 Pages: 2365-2370
  • Peer Reviewed
• [Journal Article] HP1γ links histone methylation marks to meiotic synapsis in mice (2011)
  • Author(s): Takada Y, Naruse C, Costa Y, Shirakawa T, Tachibana M, Sharif J, Kezuka-Shiotani F, Kakiuchi D, Masmnoto H, Shinkai Y, Ohbo K, Peters AH, Turner JM, Asano M, Koseki H
  • Journal Title: Development Volume: 138 Issue: 19 Pages: 4207-4217
  • DOI: 10.1242/dev.064444
  • Peer Reviewed
• [Presentation] Changing epigenetic modification and chromocenter formation associating with spermatogonia differentiation (2012)
  • Author(s): K. Ohbo, T.Shirakawa and H.Koseki
  • Organizer: 10th.EMBL Conference, Transcription and Chromatin
  • Place of Presentation: EMBL, Heidelbeerg, Germany
  • Year and Date: 2012-08-26
• [Presentation] Analysis of epigenetic changes associated with spermatogonia stem cell differentiation (2012)
  • Author(s): K.Ohbo
  • Organizer: The 58th/60th NIBB Conference, Germline Specification, Sex, and Stem Cells
  • Place of Presentation: 岡崎カンファランスセンター(愛知県)
  • Year and Date: 2012-07-21
• [Presentation] マウス精子形成過程における幹細胞と前駆細胞をゲノム修飾からとらえる (2012)
  • Author(s): 大保和之、白川峰征、神里亮人、中島久仁子、古関明彦
  • Organizer: 第117回日本解剖学会総会・全国学術総会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-28
• [Presentation] Analysis of epigenetic feature of spermatogonial stem cells in mice (2012)
  • Author(s): K. Ohbo, T. Shirakawa, R.Yaman-Deveci,Y.Kamizato, K.Nakajima, H.Sone, Y.Sato, S.Jafar, Y.Takada-Horisawa, S.Yoshida, K.Ura, M.Muto and H.Koseki
  • Organizer: Keystone Symposia, Epigenomics, Chromatin Dynamics
  • Place of Presentation: Keystone resort, Keystone, USA.
  • Year and Date: 2012-01-19
• [Presentation] Analysis of epigenetic changes associated with spermatogonia stem cell differentiation. (2012)
  • Author(s): Ohbo K.
  • Organizer: The 58th/60th NIBB Conference, Germline Specification, Sex, and Stem Cells.
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県）
• [Presentation] Changing epigenetic modification and chromocenter formation associating with spermatogonia differentiation (2012)
  • Author(s): Ohbo K, Shirakawa T. Koseki H
  • Organizer: 10th.EMBL Conference, Transcription and Chromatin
  • Place of Presentation: ハイデルベルグ、ドイツ
• [Presentation] Analysis of epigenetic features of spermatogonial stem cells. (2012)
  • Author(s): Ohbo K. Shirakawa T. Yaman Devece R. et al.
  • Organizer: Keystone symposia, Epigenomics
  • Place of Presentation: Keystone, USA
• [Presentation] Identification of an epigenetic checkpoint that controls the differentiation from spermatogonial stem cells to progenitor cells (2011)
  • Author(s): T. Shirakawa,R.Yaman-Deveci,Y.Kamizato, K.Nakajima, H.Sone, Y.Sato, S.Jafar, Y.Takada-Horisawa, S.Yoshida, K.Ura, M.Muto, H.Koseki, T.Suda and K.Ohbo
  • Organizer: Stem Cells in Development and Disease
  • Place of Presentation: Max-Delbrueck-Center,Berlin,Germany
  • Year and Date: 2011-09-12
• [Presentation] An epigenetic checkpoint controls the transition from a stem cell pool to a progenitor cell stage in testes (2011)
  • Author(s): K.Ohbo
  • Organizer: EMBO Conference Series on Chromatin and Epigenetics
  • Place of Presentation: EMBL, Heidelberg, Germany
  • Year and Date: 2011-06-03
• [Presentation] Identification of an epigenetic checkpoint that controls the differentiation from spermatogonial stem cells to progenitor cells. (2011)
  • Author(s): Shirakawa T. Ohbo K.
  • Organizer: Stem cells in Development and Disease
  • Place of Presentation: Berlin, Germany
• [Presentation] マウス精子形成過程における幹細胞と前駆細胞をゲノム修飾からとらえる
  • Author(s): 大保和之、白川峰征、神里亮人 他
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス（山梨県）