| Project/Area Number |
23659099
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Yokohama City University |
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Principal Investigator |
OHBO Kazuyuki 横浜市立大学, 医学研究科, 教授 (70250751)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUMOTO Naomichi 横浜市立大学, 医学研究科, 教授 (80325638)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 幹細胞 / 精巣 / Non-coding RNA / 細胞分化 / ゲノム修飾 / 生殖細胞 / 前駆細胞 / 小分子RNA |
|---|
| Research Abstract |
In mice, we found that the significant progression of heterochromatin formation occurred at the transition from stem to progenitor cells in adult male germ cells. In addition, the gene expression pattern was also significantly changed at the transition. Since it has been reported that non-coding RNA plays an important role in heterochromatin formation, resulting in changing of gene expression profiles, we analyzed and compared the expression profiles of miRNA and long non-coding RNA between stem cells and progenitor cells in adult male germ cells. We found several miRNA and long non-coding RNA that were specifically expressed in each of the cell populations.
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