| Project/Area Number |
23659100
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | Iwate Medical University |
|---|
Principal Investigator |
HITOMI Jiro 岩手医科大学, 医学部, 教授 (00218728)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ISOGAI Sumio 岩手医科大学, 医学部, 准教授 (60212966)
KIMURA Eji 岩手医科大学, 医学部, 助教 (50405750)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 発生・分化 / 脳 / 脈管形成 / 分子認識 / イメージング / 発現制御 / 脳血管 / 細胞応答 / 血管新生 |
|---|
| Research Abstract |
The cranial vascular system of zebrafish was composed of three clusters of the fli1 positive angioblasts, of which the first cluster recognized as lateral dorsal artery or primitive internal carotid artery, and the second cluster assembling at the rostral-internal region to the optic capsule, and the third cluster arising among the lateral-ventral wall of hindbrain. The rostral and caudal clusters of arterial origins express the arterial maker hey 2, while the clusters arising among the lateral-ventral wall of hindbrain express the venous maker flt4. Shh was expressed in the adjacent region of arterial angioblast-clusters, which are suggested to determine arterial endothelial fate. Time-lapse movies imaging the arterial-venous differentiation also revealed that the branches of intercarotid artery are originated by arterial angioblast clusters at the rostral, and the branches of vertebral-basilar artery are derived from the venous angioblast clusters arising among the hindbrain.
|
