Research on the mechanisms underlying developmental synaptogenesis using functional brainbow strategy

• Project/Area Number: 23659105
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Tohoku University
• Principal Investigator: YAWO Hiromu 東北大学, 大学院・生命科学研究科, 教授 (00144353)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 発生 / 分化 / シナプス形成 / トロフィック物質 / 自律神経節 / オプトジェネティクス / チャネルロドプシン / ニワトリ胚 / エレクトロポーレーション / シナプス前終末 / 遺伝子導入 / 電気穿孔法 / スライス培養

Research Abstract

The calyx-type synapse of chick ciliary ganglion (CG) has been intensively studied for decades as a model system for the synaptic development, morphology and physiology. Here, we evaluated a method for manipulating the molecular and functional organization of the presynaptic terminals of this model synapse.

Research Products

• [Journal Article] Optogenetic manipulation of neural and non-neural functions. (2013)
  • Author(s): Yawo H, Asano T, Sakai S, Ishizuka T
  • Journal Title: Development, Growth & Differentiation Volume: 55 Issue: 4 Pages: 474-490
  • DOI: 10.1111/dgd.12053
  • Peer Reviewed
• [Journal Article] Optogenetic Probing and Manipulation of the Calyx-Type Presynaptic Terminal in the Embryonic Chick Ciliary Ganglion. (2013)
  • Author(s): Egawa, R., Hososhima, S., Hou, X., Katow, H, S., Ishizuka, T. Nakamura, H., and Yawo, H.
  • Journal Title: PLoS ONE Volume: 8 Issue: 3 Pages: e59179-e59179
  • DOI: 10.1371/journal.pone.0059179
  • Peer Reviewed
• [Journal Article] Adventure beyond borders of scientific fields with optogenetics (2013)
  • Author(s): Yawo H, Koizumi A, Hegemann P
  • Journal Title: Neurosci Res Volume: 75 Issue: 1 Pages: 1-2
  • DOI: 10.1016/j.neures.2013.02.001
  • Peer Reviewed
• [Journal Article] Channelrhodopsins - their potential in gene therapy for neurological disorders (2013)
  • Author(s): Ji Z-G, Ishizuka T, Yawo H
  • Journal Title: Neurosci Res Volume: 75 Issue: 1 Pages: 6-12
  • DOI: 10.1016/j.neures.2012.09.004
  • Peer Reviewed
• [Journal Article] Targeted expression of a chimeric channelrhodopsin in zebrafish under regulation of Gal4-UAS system. (2013)
  • Author(s): Umeda K, Shoji W Sakai S, Muto A, Kawakami K, Ishizuka T, and Yawo H
  • Journal Title: Neuroscience Research Volume: 75 Issue: 1 Pages: 69-75
  • DOI: 10.1016/j.neures.2012.08.010
  • Peer Reviewed
• [Journal Article] Involvement of glutamate 97 in ion influx through photo-activated channelrhodopsin-2 (2013)
  • Author(s): Tanimoto S, Sugiyama Y, Takahashi T, Ishizuka T, Yawo H
  • Journal Title: Neurosci Res Volume: 75(1) Issue: 1 Pages: 13-22
  • DOI: 10.1016/j.neures.2012.05.008
  • Peer Reviewed
• [Journal Article] Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector (2012)
  • Author(s): Sakai S, Ueno K, Ishizuka T, Yawo H
  • Journal Title: Neuroscience Research Volume: (In press) Issue: 1 Pages: 59-64
  • DOI: 10.1016/j.neures.2012.03.009
  • Peer Reviewed
• [Journal Article] Remodeling of hippocampal network in pilocarpine-treated mice expressing synaptopHluorin in the mossy fiber terminals (2012)
  • Author(s): Ito S, Ishizuka T, Yawo H
  • Journal Title: Neurosci Res Volume: 74(1) Issue: 1 Pages: 25-31
  • DOI: 10.1016/j.neures.2012.07.003
  • Peer Reviewed
• [Journal Article] Light-evoked somatosensory perception of transgenic rats which express channelrhodopsin-2 in dorsal root ganglion cells (2012)
  • Author(s): Ji Z-G, Ito S, Honjoh T, Ohta H, Ishizuka T, Fukazawa Y, Yawo H
  • Journal Title: PLoS ONE Volume: 7 Issue: 3 Pages: e32699-e32699
  • DOI: 10.1371/journal.pone.0032699
  • Peer Reviewed
• [Journal Article] Optically Controlled Contraction of Photosensitive Skeletal Muscle cells (2012)
  • Author(s): Toshifumi Asano, et al
  • Journal Title: Biotechnology and Bioengineering Volume: 109 Issue: 1 Pages: 199-204
  • DOI: 10.1002/bit.23285
  • Peer Reviewed
• [Journal Article] Optically controlled contraction of photosensitive skeletal muscle cells (2012)
  • Author(s): Asano T, Ishizuka T, Yawo H
  • Journal Title: Biotechnol Bioeng Volume: 109 Issue: 4 Pages: 199-204
  • DOI: 10.1016/j.neures.2011.08.013
  • Peer Reviewed
• [Journal Article] Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector. (2012)
  • Author(s): Sakai S, Ueno K, Ishizuka T, Yawo H
  • Journal Title: Neurosci Res. Volume: in press
  • Peer Reviewed
• [Presentation] Optogenetic manipulation of presynaptic activity in chick ciliary ganglion during synapse elimination (2013)
  • Author(s): Shoko Hososhima, Toru Ishizuka, Hiromu Yawo
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] An experimental platform to study physiology, morphology and development of the vertebrate presynaptic terminal with genetic manipulations (2013)
  • Author(s): Ryo Egawa, Shoko Hososhima, Saki Tanimoto, Toru Ishizuka, Harukazu Nakamura, Hiromu Yawo
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] 光操作システムの開発とオプトジェネティクス(光遺伝学)への応用 (2012)
  • Author(s): 八尾寛
  • Organizer: 第32回東邦大学生命科学シンポジウム
  • Place of Presentation: 船橋市
  • Year and Date: 2012-10-31
• [Presentation] 杯状シナプスにおけるシナプス除去機構への光遺伝学的アプローチ (2012)
  • Author(s): 細島頌子、石塚徹、八尾寛
  • Organizer: 第44回東北生理談話会
  • Place of Presentation: 山形
  • Year and Date: 2012-10-27
• [Presentation] 光受容体チャネル、チャネルロドプシンのイオン流はGd3+によりブロックされる (2012)
  • Author(s): 谷本早希、石塚徹、八尾寛
  • Organizer: 第44回東北生理談話会
  • Place of Presentation: 山形
  • Year and Date: 2012-10-27
• [Presentation] 次世代シナプスモデルを用いた発達期シナプス刈込機構の研究 (2012)
  • Author(s): 江川遼、八尾寛
  • Organizer: 東北大学脳科学GCOE異分野融合研究報告会
  • Place of Presentation: 仙台
  • Year and Date: 2012-03-02
• [Presentation] Optogenetic manipulation of presynaptic activity in the ciliary ganglion of chick embryo (2012)
  • Author(s): Shoko Hososhima, Toru Ishizuka, Hiromu Yawo
  • Organizer: 7th International chick meeting
  • Place of Presentation: Nagoya, Japan
• [Presentation] チャネルロドプシンを発現した骨格筋管細胞における筋収縮と成熟の光操作 (2012)
  • Author(s): 浅野豪文,石塚徹,八尾寛
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
• [Presentation] An experimental platform for the study of presynaptic development, morphology and physiology - gene manipulation of the vertebrate model synapse, Cold Spring Harbor Laboratory Meeting (2012)
  • Author(s): Egawa R, Hososhima S, Tanimoto S, Ishizuka T, Nakamura H, Yawo H
  • Organizer: Axon Guidance, Synapse Formation and Regeneration"
  • Place of Presentation: Cold Spring Harbor, USA
• [Presentation] ニワトリ胚毛様体神経節における杯状シナプスの発達プロセス-Brainbow法による解析 (2012)
  • Author(s): Egawa,R.,Hososhima,S.,Ishizuka,T.,Nakamura,H.,andYawo,H
  • Organizer: 東北脳科学ウィンタースクール
  • Place of Presentation: 仙台・蔵王
• [Presentation] Involvement of L-type Ca2+ channels in the postnatal neurogenesis (2012)
  • Author(s): Teh DBL, Ishizuka T, Yawo H
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] An experimental platform for the study of presynaptic development, morphology and physiology - gene manipulation of the vertebrate model synapse (2012)
  • Author(s): Egawa R, Hososhima S, Tanimoto S, Ishizuka T, Nakamura H, Yawo H
  • Organizer: Cold Spring Harbor Laboratory Meeting “Axon Guidance, Synapse Formation and Regeneration”
  • Place of Presentation: Cold Spring Harbor, USA
• [Presentation] Optogenetic manipulation of presynaptic activity in the ciliary ganglion of chick embryo (2012)
  • Author(s): Shoko Hososhima, Toru Ishizuka, Hiromu Yawo
  • Organizer: 7th International chick meeting
  • Place of Presentation: 名古屋
• [Presentation] オプトジェネティクスを用いた培養骨格筋の光操作 (2011)
  • Author(s): 浅野豪文、石塚徹、八尾寛
  • Organizer: 生体情報インターフェース創生のためのフォトニクス研究‐共同プロジェクト研究会
  • Place of Presentation: 仙台
  • Year and Date: 2011-12-13
• [Presentation] ニワトリ胚毛 様体神経節における杯状シナプスの発 達プロセス-Brainbow 法による解析 (2011)
  • Author(s): Egawa, R., Hososhima, S., Ishizuka, T., Nakamura, H., and Yawo, H
  • Organizer: 生 化学若い研究者の会 若手シンポジウ ム
  • Place of Presentation: 仙台
  • Year and Date: 2011-12-10
• [Presentation] Distribution of the actin-binding protein, coactosin in the chick ciliary ganglion during embryonic synaptogenesis (2011)
  • Author(s): 細島頌子、侯旭濱、仲村春和、石塚徹、八尾寛
  • Organizer: 3rd UCL-Tohoku symposium
  • Place of Presentation: London, UK
  • Year and Date: 2011-10-19
• [Presentation] チャネルロドプシンを用いた光刺激による筋収縮制御 (2011)
  • Author(s): 浅野豪文、石塚徹、八尾寛
  • Organizer: 第1回オプトジェネティクス講習会
  • Place of Presentation: 仙台
  • Year and Date: 2011-09-23
• [Presentation] Resonant rhythms generated by neurons and network- opto-current-clamp with optimizedchannelrhodopsins (2011)
  • Author(s): Yawo H, Wen L, Sakai S, Wang H, Tanimoto S, Egawa R, Ishizuka T
  • Organizer: 第84回日本生化学会 大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-21
• [Presentation] Expression of the actin-bindingproteins, coactosin and drebrin, in the developing chick ciliary ganglion (2011)
  • Author(s): 細島頌子、侯旭濱、仲村春和、石塚徹、八尾寛
  • Organizer: 第34回 日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] The molecular determinants involved in ion flux regulation of channelrhodopsin-2 (2011)
  • Author(s): Tanimoto S, Wang H, Sugiyama Y, Ishizuka T, Yawo H
  • Organizer: 第34回日本神経科 学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] The molecular determinants involved in ion flux regulation of channelrhodopsins (2011)
  • Author(s): Tanimoto S, Wang H, Sugiyama Y, Ishizuka T, Yawo H
  • Organizer: 8th IBRO World Congress of Neuroscience
  • Place of Presentation: Florence, Itary
  • Year and Date: 2011-07-17
• [Presentation] 新世代チャネルロドプシンによるオプトジェネティクス.神経組織の成長・再生・移植研究会 (2011)
  • Author(s): 八尾寛,石塚徹
  • Organizer: 第26回学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-25
• [Presentation] 光受容イオンチャネル、チャネルロドプシンの光受容-チャネル構造・機能連関 (2011)
  • Author(s): 八尾寛
  • Organizer: CBI学会第317回研究講演会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-27
• [Presentation] Localization of the actin binding protein, coactosin that expressed in the developing chick ciliary ganglion (2011)
  • Author(s): 細島頌子、侯旭濱、仲村春和、石塚徹、八 尾寛
  • Organizer: 第44回 日本発生生物学会年会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-19
• [Presentation] ニワトリ胚毛様体 神経節における杯状シナプスの形成- Brainbow 法による解析 (2011)
  • Author(s): Egawa R, Hososhima S, Ishizuka T, Nakamura H, Yawo H
  • Organizer: The 34th annualMeeting of the Japan Neuroscience Society (NEURO2011)
  • Place of Presentation: 横浜
• [Presentation] Synaptogenesis in the embryonic chick ciliary ganglion - a brainbow study. OIST International Workshop (2011)
  • Author(s): Egawa R, Hososhima S, Ishizuka T, Nakamura H, Yawo H
  • Organizer: Molecular & structural organization of presynaptic function and plasticity
  • Place of Presentation: Okinawa
• [Presentation] Development of the calyx-type synapses in the embryonic chick ciliary ganglion - a brainbow study (2011)
  • Author(s): Egawa R, Hososhima S, Ishizuka T, Nakamura H, Yawo H
  • Organizer: 8th IBRO WORLD CONGRESS OFNEUROSCIENCE
  • Place of Presentation: Florence, Italy
• [Presentation] Development of the calyx-type synapses in the embryonic chick ciliary ganglion - a brainbow study. (2011)
  • Author(s): Egawa R, Hososhima S, Ishizuka T, Nakamura H, Yawo H
  • Organizer: 8th IBRO WORLD CONGRESS OF NEUROSCIENCE
  • Place of Presentation: Florence (Italy)
• [Presentation] Synaptogenesis in the embryonic chick ciliary ganglion - a brainbow study. (2011)
  • Author(s): Egawa R, Hososhima S, Ishizuka T, Nakamura H, Yawo H
  • Organizer: OIST International Workshop "Molecular & structural organization of presynaptic function and plasticity"
  • Place of Presentation: Okinawa
• [Presentation] Localization of the actin binding protein, coactosin that expressed in the developing chick ciliary ganglion. (2011)
  • Author(s): 細島頌子、侯旭濱、仲村春和、石塚徹、八尾寛
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄
• [Presentation] ニワトリ胚毛様体神経節における杯状シナプスの形成－Brainbow法による解析。 (2011)
  • Author(s): Egawa R, Hososhima S, Ishizuka T, Nakamura H, Yawo H
  • Organizer: The 34th annual Meeting of the Japan Neuroscience Society (NEURO2011)
  • Place of Presentation: 横浜
• [Presentation] Expression of the actin-binding proteins, coactosin and drebrin, in the developing chick ciliary ganglion. (2011)
  • Author(s): 細島頌子、侯旭濱、仲村春和、石塚徹、八尾寛
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Distribution of the actin-binding protein, coactosin in the chick ciliary ganglion during embryonic synaptogenesis. (2011)
  • Author(s): 細島頌子、侯旭濱、仲村春和、石塚徹、八尾寛
  • Organizer: 3rd UCL-Tohoku symposium
  • Place of Presentation: London （英国）
• [Presentation] 光操作システムの開発とオプトジェネティクス(光遺伝学)への応用
  • Author(s): 八尾 寛
  • Organizer: 第32回 東邦大学生命科学シンポジウム
  • Place of Presentation: 船橋市
  • Invited
• [Presentation] 杯状シナプスにおけるシナプス除去機構への光遺伝学的アプローチ
  • Author(s): 細島 頌子、石塚 徹、八尾 寛
  • Organizer: 第44回東北生理談話会
  • Place of Presentation: 山形
• [Book] Muscle Optogenetics: Controlling muscle functions with light. In: Muscle Cells (Ed, Benigno Pezzo) (2013)
  • Author(s): Asano T, Ishizuka T, Yawo H
  • Publisher: Nova Science Publisher Inc.Hauppauge NY
• [Book] Muscle Optogenetics: Controlling muscle functions with light. In: Muscle Cells (Ed, Benigno Pezzo) (2013)
  • Author(s): Asano T, Ishizuka T, Yawo H
  • Publisher: Nova Science Publisher Inc., Hauppauge NY
• [Book] Whole-Cell Patch Method" Patch-Clamp Techniques: From Beginning to Advanced Protocols (Springer Protocols Handbooks) (Ed. Yasunobu Okada) (2012)
  • Author(s): Hiromu Yawo
  • Publisher: Springer Verlag, Japan
• [Book] "Whole-Cell Patch Method" Patch-Clamp Techniques: From Beginning to Advanced Protocols (Springer Protocols Handbooks) (Ed. Yasunobu Okada) (2012)
  • Author(s): Hiromu Yawo
  • Total Pages: 27
  • Publisher: Springer Verlag, Japan
• [Book] 「ホールセル記録法」最新パッチクランプ実験技術法（岡田泰伸 編） (2011)
  • Author(s): 八尾 寛
  • Total Pages: 17
  • Publisher: 吉岡書店
• [Remarks] 1.日本経済新聞(朝刊,科学欄),光が照らす脳の迷宮.2012年7月1日. 新聞報道
• [Remarks] 2.日経産業新聞、皮膚で光感じるネズミ‐触角の仕組み解明に活用、2012年3月21日 新聞報道
• [Remarks] 3.朝日新聞(朝刊、科学欄)、手足の皮膚で光を感知、2012年3月15日 新聞報道
• [Remarks] 4.河北新報(朝刊)、皮膚で光を感知、東北大グループがマウス使い発見応用期待、2012年03月09日 新聞報道
• [Remarks]
  • URL: http://neuro.med.tohoku.ac.jp/
• [Remarks]
  • URL: http://ja.sendaibrain.org/member/yawo/index.html
• [Remarks]
  • URL: http://www.lifesci.tohoku.ac.jp/research/field_list/neurosciences/t_yawo
• [Patent(Industrial Property Rights)] ラット脳内光誘発けいれんモデル (2012)
  • Inventor(s): 大沢 伸一郎,岩崎 真樹,虫明 元, 八尾 寛 ,冨永 悌二,古澤 義人
  • Industrial Property Rights Holder: 東北大学
  • Filing Date: 2012-09-12
• [Patent(Industrial Property Rights)] ラット脳内光誘発けいれんモデル (2011)
  • Inventor(s): 大沢 伸一郎,岩崎 真樹,虫明 元, 八尾 寛 ,冨永 悌二,古澤 義人
  • Industrial Property Rights Holder: 東北大学
  • Industrial Property Number: 2011-198126
  • Filing Date: 2011-09-12
• [Patent(Industrial Property Rights)] ラット脳内光誘発けいれんモデル (2011)
  • Inventor(s): 大沢伸一郎,岩崎真樹,虫明元,八尾寛,冨永悌二,古澤義人
  • Industrial Property Rights Holder: 東北大学
  • Industrial Property Number: 2011-198126
  • Filing Date: 2011