Biosynthesis of heparin proteoglycan (serglycin)

• Project/Area Number: 23659108
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: YANAGISHITA Masaki 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (70132793)
• Co-Investigator(Kenkyū-buntansha): KATARZYNA ANNA Podyma-Inoue 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (90302877)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 血液凝固 / レオロジー / heparin / heparan sulfate / glycosaminoglycan / proteoglycan

Research Abstract

Heparin is a biological product having anticoagulant activity. It is widely used in heart-lung surgeries, hemodialysis, and in the treatment of arterial/venous thromboses. Biosynthetic processes involved in the processing of heparin, especially that of carbohydrate chains after completion in Golgi until the storage of the final products in secretion granules, have not been elucidated. The current research attempted to analyze this processes in detail, and has reached a conclusion that the “heparanase” is the key enzyme in this process and the newly generated non-reducing ends of heparin chains weresuggested to have specific functions.

Research Products

• [Journal Article] Degradation of MUC7 and MUC5B in Human Saliva (2013)
  • Author(s): Sachiko Takehara, Masaki Yanagishita, Katarzyna Anna Podyma-Inoue and Yoko Kawaguchi
  • Journal Title: PLOS ONE Volume: (in press)
• [Journal Article] (2013)
  • Author(s): Miki Hara-Yokoyama, Kazue Terasawa, Shizuko Ichinose, Akihiko Watanabe, Katarzyna A. Podyma-Inoue, Kazunari Akiyoshi, Yasuyuki Igarashi and Masaki Yanagishita
  • Journal Title: Bioorganic & Madicinal Chemistry letter Volume: (in press)
• [Journal Article] (2012)
  • Author(s): Miki Hara-Yokoyama, Mutsuko Kukimoto-Niino, Kazue Terasawa, Satoru Harumiya, Katarzyna A. Podyma-Inoue, Nobumasa Hino, Kensaku Sakamoto, Satsuki Itoh, Noritaka Hashii, Yoko Hiruta, Nana Kawasaki, Chiemi Mishima- Tsumagari, Yoko Kaitsu, Tomoko Matsumoto, Motoaki Wakiyama, Mikako Shirouzu, Takeshi Kasama, Hiroshi Takayanagi, Nakako Utsunomiya-Tate, Kiyoshi Takatsu, Toshiaki Katada, Yoshio Hirabayashi, Shigeyuki Yokoyama and Masaki Yanagishita
  • Journal Title: Structure Volume: 20 Pages: 1585-1595
• [Journal Article] The proteoglycan repertoire of lymphoid cells (2012)
  • Author(s): Bodil Fadnes, Anne Husbekk, Gunbjorg Sveing, Oystein Rekdal, Masaki Yanagishita, Svein O. Kolset and Lars Uhlin-Hansen
  • Journal Title: Glycoconjugate Volume: J.29 Pages: 513-523
• [Journal Article] Syndecans Reside in Sphingomyelin-enriched Low Density Fractions of the Plasma membrane Isolated from a Parathyroid Cell Line (2012)
  • Author(s): Katarzyna A. Podyma-Inoue, Miki Yokoyama, Tomoko Kimura and Masaki Yanagishita
  • Journal Title: PLOS ONE Volume: 7:3
• [Journal Article] Syndecans reside in Sphyngomyelin-enriched Low-density Fractions of the Plasma Membrane Isolated from a Parathyroid Cell Line (2012)
  • Author(s): Katarzyna A. Podyma-Inoue
  • Journal Title: PLoS One Volume: 7 Issue: 3 Pages: e32351-e32351
  • DOI: 10.1371/journal.pone.0032351
  • Peer Reviewed
• [Journal Article] Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities (2012)
  • Author(s): Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue K. A., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Ta
  • Journal Title: Structure Volume: 20 Issue: 9 Pages: 1585
  • DOI: 10.1016/j.str.2012.06.017
  • Peer Reviewed
• [Journal Article] The proteoglycan repertoire of lymphoid cells (2012)
  • Author(s): Bodil Fadnes
  • Journal Title: Glycoconjugate J. Volume: 29 Issue: 7 Pages: 513-523
  • DOI: 10.1007/s10719-012-9427-9
  • Peer Reviewed
• [Journal Article] Syndecans Reside in Sphyngomyelin-enriched Low-density Fractions of the Plasma membrane Isolated from a Parathyroid Cell Line (2012)
  • Author(s): Katarzyna A. Podyma-Inoue, Miki Hara-Yokoyama, Tamayuki Shinomura, Tomoko Kimura and Masaki Yanagishita
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] Periodontal pockets as the pathological setting for HMGB1 release (2011)
  • Author(s): N. Ebe, M. Hara-Yokoyama, K. Iwasaki, S. Iseki, K. Terasawa, K. A. Podyma-Inoue, M.Yanagishita and Y. Izumi
  • Journal Title: J. Dental Res Volume: 90 Pages: 235-240
• [Journal Article] Periodontal pockets as the pathological setting for HMGB1 release (2011)
  • Author(s): N. Ebe, M. Hara-Yokoyama, K. Iwasaki, S. Iseki, K. Terasawa, K. A. Podyma-Inoue, M. Yanagishita and Y. Izumi
  • Journal Title: J. Dental Res. Volume: 90 Pages: 235-240
  • Peer Reviewed
• [Presentation] Introduction, turnover and degradation of hyaluronan (2013)
  • Author(s): Masaki Yanagishita
  • Organizer: 2013 International Society for Hyaluronan Sciences Meeting
  • Place of Presentation: Oklahoma City, OK, USA
• [Presentation] プロテオグライカン、ヒアルロン酸研究の最新トピックス (2012)
  • Author(s): 柳下正樹
  • Organizer: 第19回プロテオグライカンフォーラム
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2012-02-11
• [Presentation] Proteolytic Degradation of Human Salivary MUC5B and MUC7 (2012)
  • Author(s): S. Takehara, M. Yanagishita, K. A. Podyma-Inoue, M. Ueno, K. Shinada and Y. Kawaguchi
  • Organizer: Annual Meeting of International Association for Dental Research
  • Place of Presentation: San Diego
• [Presentation] Association of heparan sulphate proteoglycan sith trypsin-accessible membrane domains (2012)
  • Author(s): Katarzyna A. Podyma-Inoue, Miki Yokoyama and Masaki Yanagishita
  • Organizer: Gordon Research Conference on Poteoglycan
  • Place of Presentation: Andover, NH, USA
• [Presentation] Expression of GIP and itsreceptor in the rat major salivary glands (2012)
  • Author(s): Privatananupunt Jutiporn, WatariIppei, Podyma-Inoue Katarzyna A.,Kubono Mariko, Hattori Ikuko, Honda Koji, Ishida Yuji, Yanagishita Masaki, and Ono Takashi
  • Organizer: 71st Annual Meeting of the JapaneseOrthodontic Society
  • Place of Presentation: Morioka
• [Presentation] Association of heparan sulfate proteoglycan with trypsin-accessible membrane domains (2012)
  • Author(s): Katarzyna A. Podyma-Inoue
  • Organizer: Gordon Research Conference on Poteoglycan
  • Place of Presentation: Andover, USA
• [Presentation] Expression of GIP and its receptor in the rat major salivary glands (2012)
  • Author(s): Privatananupunt Jutiporn
  • Organizer: 71st Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: 盛岡
• [Presentation] Proteolytic Degradation of Human Salivary MUC5B and MUC7, Symposium (2011)
  • Author(s): S. Takehara, M. Yanagishita, K. A. Podyma-Inoue, M. Ueno, K. Shinada and Y. Kawaguchi
  • Organizer: Toward networking dental professionals in Asia
  • Place of Presentation: Tokyo
  • Year and Date: 2011-10-06
• [Presentation] Life is not only wine and roses"? - climbing up Japanese social ladder (2011)
  • Author(s): Katarzyna Inoue
  • Organizer: 第84回日本生化学会ランチョンワークショップ
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] CD38の触媒活性と膜ドメイン局在化を両立させている細胞表面でのアセンブリの構造基盤 (2011)
  • Author(s): Yokoyama Miki, Yokoyama Shigeyuki, Yanagishita Masaki
  • Organizer: 2011日本免疫学会総会
  • Place of Presentation: 日幕張メッセ
• [Presentation] 酪酸による酸化ストレス誘導時に放出されるHMGB1の解析 (2011)
  • Author(s): 江部典子、横山三紀、寺澤和恵、柳下正樹、和泉雄一
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 大阪
• [Presentation] Analysis of neurotransmitters/neuromodulators released under low gravity usingmicrodialysis technique (2011)
  • Author(s): Katarzyna A. Inoue, Kimiya Norikiyo, Jorge L. Zaredo, Akira Masuda, Shuji Aou and Yasuhiro Kumei
  • Organizer: 第34回日本神経科学会
  • Place of Presentation: 横浜
• [Presentation] Characterization of Membrane Rafts Enriched in Cell Surface Haparan Sulfate Proteoglycans Isolated from a RatParathyroid Cell Line (2011)
  • Author(s): Katarzyne A. Podyma-Inoue, Miki Yokoyama and Masaki Yanagishita
  • Organizer: 第84回日本生化学大会
  • Place of Presentation: 京都
• [Presentation] リンパ球表面抗原CD38の4量体化を反映したN型糖鎖プロセシング (2011)
  • Author(s): 横山三紀、新野睦子、寺澤和恵、橋井則貴、蛭田葉子、川崎ナナ、脇山素明、白水美香子、横山茂之、柳下正樹
  • Organizer: 第30回日本糖質学会
  • Place of Presentation: 長岡
• [Presentation] GM2/GD2合成酵素欠損マウスにおける血液-精巣関門の異常 (2011)
  • Author(s): 横山三紀、市野瀬志津子、市野瀬省三、多田晃弘、寺澤和恵、吉垣純子、只野-有冨桂子、古川鋼一、柳下正樹、岩渕和久
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
• [Presentation] Characterization of Membrane Rafts Enriched in Cell Surface Haparan Sulfate Proteoglycans Isolated from a Rat Parathyroid Cell Line. (2011)
  • Author(s): Katarzyne A. Podyma-Inoue, Miki Yokoyama and Masaki Yanagishita
  • Organizer: 第84回日本生化学大会、
  • Place of Presentation: 京都
• [Presentation] Proteolytic Degradation of Human Salivary MUC5B and MUC7, (2011)
  • Author(s): S. Takehara, M. Yanagishita, K. A. Podyma-Inoue, M. Ueno, K. Shinada and Y. Kawaguchi
  • Organizer: Symposium "Toward networking dental professionals in Asia"
  • Place of Presentation: Tokyo
• [Presentation] "Life is not only wine and roses"? - climbing up Japanese social ladder (2011)
  • Author(s): Katarzyna Inoue
  • Organizer: 第84回日本生化学会ランチョンワークショップ(招待講演)
  • Place of Presentation: 京都
• [Presentation] Analysis of neurotransmitters/neuromodulators released under low gravity using microdialysis technique (2011)
  • Author(s): Katarzyna A. Inoue, Kimiya Norikiyo, Jorge L. Zaredo, Akira Masuda, Shuji Aou and Yasuhiro Kumei
  • Organizer: 第34回日本神経科学会
  • Place of Presentation: 横浜