Establishment of human pluripotent stem cell-derived pace-making cells using physiological approach and its application to the regenerative medicine
| Project/Area Number |
23659112
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Tottori University |
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Principal Investigator |
HISATOME Ichiro 鳥取大学, 大学院・医学系研究科, 教授 (60211504)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAYOSHI Yasuaki 鳥取大学, 医学研究科, 准教授 (90249946)
YAMAMOTO Yasutaka 鳥取大学, 医学研究科, 特任准教授 (20362882)
MIAKE Junichiro 鳥取大学, 医学部, 講師 (40372677)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | human ES cells / HCN4 / pacemaking cells / ヒトES細胞 / ヒトiPS細胞 / HCN4搭載BACベクター / ヒトＥＳ細胞 / ペースメーカ細胞 |
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| Research Abstract |
We attempted to establish the human ES cells harboringHCN4-GFP BAC vector to isolate human pluripotent stem cell-derived pace-making cells using physiological approach. We construct BAC vector harboring the knocked GFP gene into the promoter region of HCN4 gene and introduced it into human ES cells (KhES-1) to establish the several clones including clone #1. These clones expressed the pluripotent gene markers and normal karyo type of chromosome. The embryoid body derived from clone#1 to differentiate cardiac cells included the HCN4-GFP positive cells at 5% out of total human ES cells. HCN4-GFP positive cells expressed cardiac contractile proteins and HCN4 channels. HCN4-GFP positive cells expressed the several ion channels being responsible for their automaticity. The Ca transient derived from the aggregation of HCN4-GFP positive cells propagated into the HL-1 atrial cell-sheet. Taken together, we establish the human ES cells harboringHCN4-GFP BAC vector and successfully isolate the HCN4-GFP positive pacemakingcells.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] A vasodilating β1 blocker celiprolol inhibits muscular release of uric acid precursor in patients with essential hypertension.2013
Author(s)
Mizuta E, Utami SB, Ohtahara A, Endo S, Mishima M, Hasegawa A, Yamada K, Kato M, Yamamoto K, Ogino K, Ninomiya H, Miyazaki S, Hamada T, Taniguchi SI, Cheng J, Hisatome I.
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Journal Title
Horm Metab Res.
Volume: 45(1)
Pages: 69-73
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[Journal Article] Stabilization of Kv1.5 channel protein by bepridil through its action as a chemical chaperone2012
Author(s)
Suzuki S, Kurata Y, Li P, Notsu T, Hasegawa A, Ikeda N, Kato M, Miake J, Sakata S, Shiota G, Yoshida A, Ninomiya H, Higaki K, Yamamoto K, Shirayoshi Y, Hisatome I.
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Journal Title
Eur J Pharmacol.
Volume: 696(1-3)
Pages: 28-34
NAID
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[Journal Article] Electrophysiological properties of prion-positive cardiac progenitors derived from murine embryonic stem cells.2012
Author(s)
Fujii H, Ikeuchi Y, Kurata Y, Ikeda N, Bahrudin U, Li P, Nakayama Y, Endo R, Hasegawa A, Morikawa K, Miake J,Yoshida A, Hidaka K, Morisaki T, Ninomiya H, Shirayoshi Y, Yamamoto K, Hisatome I.
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Journal Title
Circ J.
Volume: 76(12)
Pages: 2875-83
NAID
Related Report
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[Journal Article] Effects of cilnidipine on serum uric acid level and urinary nitrogen monoxide excretion in patients with hypertension.2012
Author(s)
Hamada T, Yamada K, Mizuta E, Watanabe A, Osaki T, Ishida K, HasegawaA, Sakata S, Mishima M, Ogino K, Nosaka Y, Miyazaki S, Ohtahara A, Ninomiya H, Kato M, Yoshida A, Taniguchi S, Yamamoto K, Hisatome I.
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Journal Title
Clin Exp Hypertens
Volume: 34(7)
Pages: 470-3
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[Journal Article] Reciprocal Control of hERG Stability by Hsp70 and Hsc70 with Implication for Restoration of LQT2 Mutant Stability2011
Author(s)
Peili Li, Haruaki Ninomiya, Yasutaka Kurata, Masaru Kato, Junichiro Miake, Yasutaka Yamamoto, Osamu Igawa, Akira Nakai, Katsumi Higaki, Futoshi Toyoda, Jie Wu, Minoru Horie, Hiroshi Matsuura, Akio Yoshida, Yasuaki Shirayoshi, Masayasu Hiraoka, Ichiro Hisatome.
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Journal Title
Circ Res.
Volume: 108(4)
Pages: 458-68
NAID
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[Journal Article] Transcriptional activation of SAP97 by HSF-1 stabilizes Kv1.5 in HL-1 cells2011
Author(s)
YK Ting, K Morikawa, Y Kurata, PL Li, U Bahrudin, E Mizuta, M Kato,J Miake, Y Yamamoto, A Yoshida, M Murata, T Inoue, A Nakai, G Shiota, K Higaki, E Nanba, H Ninomiya, Y Shirayoshi, I Hisatome.
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Journal Title
Br J Pharmacol
Volume: 162(8)
Pages: 1832-1842
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[Journal Article] Chemical chaperone therapy: chaperone effect on mutant enzyme and cellular pathophysiology in β-galactosidase deficiency2011
Author(s)
Higaki K, Li L, Bahrudin U, Okuzawa S, Takamura A, Yamamoto K, Adachi K, Paraguison RC, Takai T, Ikehata H, Tominaga L, Hisatome I, Iida M, Ogawa S, Matsuda J, Ninomiya H, Sakakibara Y, Ohno K, Suzuki Y, Nanba E.
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Journal Title
Hum Mutat
Volume: 32(7)
Pages: 843-852
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[Journal Article] c-Jun N-terminal kinase activation by oxidative stress suppresses retinoid signaling through proteasomal degradation of retinoic acid receptor α protein in hepatic cells.2011
Author(s)
Hoshikawa Y, Kanki K, Ashla AA, Arakaki Y, Azumi J, Yasui T, Tezuka Y, Matsumi Y, Tsuchiya H, Kurimasa A, Hisatome I, Hirano T, Fujimoto J, Kagechika H, Shomori K, Ito H, Shiota G.
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Journal Title
Cancer Sci.
Volume: 120(5)
Pages: 934-41
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[Journal Article] Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation2011
Author(s)
Ikeda R, Ishii K, Hoshikawa Y, Azumi J, Arakaki Y, Yasui T, Matsuura S, Matsumi Y, Kono Y, Mizuta Y, Kurimasa A, Hisatome I, Friedman SL, Kawasaki H, Shiota G.
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Journal Title
Inflamm Res
Volume: 60(6)
Pages: 597-604
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[Journal Article] Impairment of ubiquitin-proteasome system by E334K cMyBPC modifies channel proteins, leading to electrophysiological dysfunction2011
Author(s)
Bahrudin U, Morikawa K, Takeuchi A, Kurata Y, Miake J, Mizuta E, Adachi K, HigakiK, Yamamoto Y, Shirayoshi Y, Yoshida A, Kato M, Yamamoto K, Nanba E, Morisaki H, Morisaki T, Matsuoka S, Ninomiya H, Hisatome I.
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Journal Title
J Mol Biol
Volume: 413(4)
Pages: 857-78
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[Journal Article] Enhancing effects of salicylate on quinidine-induced block of human wild type and LQT3 related mutant cardiac Na+ channels2011
Author(s)
Urashima T, Kurata Y, Miake J, Kato M, Ogura K, Yano A, Adachi M, Tanaka Y, Yamada K, Hamada T, Mizuta E, Kuwabara M, Kato M, Yamamoto Y, Ogino K, Yoshida A, Shirayoshi Y, Hisatome I.
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Journal Title
Biomed Res
Volume: 32(5)
Pages: 303-12
NAID
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[Journal Article] AMP deaminase 3 plays a critical role in remote reperfusion lung injury.
Author(s)
Li P, Ogino K, Hoshikawa Y, Morisaki H, Toyama K, Morisaki T, Morikawa K, Ninomiya H, Yoshida A, Hashimoto K, Shirayoshi Y, Hisatome I.
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Journal Title
Biochem Biophys Res Commun.
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