Elucidation of mechanism for production of causal factor of vasospasm

• Project/Area Number: 23659113
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Yamaguchi University
• Principal Investigator: KOBAYASHI Sei 山口大学, 医学(系)研究科(研究院), 教授 (80225515)
• Co-Investigator(Kenkyū-buntansha): KISHI Hiroko 山口大学, 大学院医学系研究科, 准教授 (40359899)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 病態生理 / 血管攣縮 / 血管異常収縮 / 血管病 / 酸化ストレス / 活性酸素

Outline of Final Research Achievements

The purpose of this study is to clarify the molecular mechanism of causal factor of vasospasm, SPC. Surprisingly, SPC was not produced by the enzymatic reaction, but by the non-enzymatic reaction. We discovered that OH radical plays an essential role of the non-enzymatic production of SPC.

Research Products

• [Journal Article] Fatty acid-binding protein 7 regulates function of caveolae in astrocytes through expression of caveolin-1 (2015)
  • Author(s): Yoshiteru Kagawa, Yuki Yasumoto, Kazem Sharifi, Majid Ebrahimi, Ariful Islam, Hirofumi Miyazaki, Yui Yamamoto, Tomoo Sawada, Hiroko Kishi, Sei Kobayashi, Motoko Maekawa, Takeo Yoshikawa, Eiichi Takaki Akira Nakai, Hiroshi Kogo, Toyoshi Fujimoto, Yuji Owada
  • Journal Title: Glia Volume: 63 Pages: 780-794
  • Peer Reviewed
• [Journal Article] Phosphorylation of the Kinase Domain Regulates Autophosphorylation of Myosin IIIA and Its Translocation in Microvilli (2014)
  • Author(s): Byung Chull An, Tsuyoshi Sakai, Shigeru Komaba, Hiroko Kishi, Sei Kobayashi, Jin Young Kim, Reiko Ikebe, Mistuo Ikebe
  • Journal Title: Biochemistry Volume: 53 Issue: 49 Pages: 7835-7845
  • DOI: 10.1021/bi501247z
  • Peer Reviewed
• [Journal Article] 食品成分による血管病予防の新展開 (2012)
  • Author(s): 加治屋勝子、岸博子、高田雄一、張影、木村友彦、宮成健司、萩原弘、小林誠
  • Journal Title: FFIジャーナル Volume: 217 Pages: 284-289
  • NAID: 40019419438
  • Peer Reviewed
• [Journal Article] Sphingosylphosphorylcholine induces stress fiber formation via activation of Fyn-RhoA-ROCK signaling pathway in fibroblasts (2012)
  • Author(s): Dan xu
  • Journal Title: Cellular Signaling Volume: 24 Issue: 1 Pages: 282-289
  • DOI: 10.1016/j.cellsig.2011.09.013
  • Peer Reviewed
• [Presentation] N-terminus of paxillin regulates actin stress fiber formation by binding to the active Fyn (2015)
  • Author(s): Ying Zhang, Hiroko Kishi, Kenji Miyanari, Tomohiko Kimura, Ryodai Takagaki, Bochao Lyu, Sei Kobayashi
  • Organizer: 第120回日本解剖学会総会全国学術集会・第92回日本生理学会大会 合同大会
  • Place of Presentation: 神戸国際会議場・展示場 (神戸市)
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Discovery of novel Salacia-derived components which specifically inhibit the ROK-mediated Ca2+- sensitization of vascular smooth muscle contraction (2015)
  • Author(s): Kenji Miyanari, Kazutaka Nojimoto, Katsuko Kajiya, Yuichi Takada, Tomohiko Kimura, Ying Zhang, Hiroko Kishi, Sei Kobayashi
  • Organizer: 第120回日本解剖学会総会全国学術集会・第92回日本生理学会大会 合同大会
  • Place of Presentation: 神戸国際会議場・展示場 (神戸市)
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] アクチン・ストレスファイバー形成における、新規シグナル分子パキシリンと活性型Fynチロシンキナーゼ との相互作用の重要性 (2014)
  • Author(s): 張 影、岸 博子、宮成健司、木村友彦、高柿了大、呂 博超、小林 誠
  • Organizer: 第56回日本平滑筋学会総会
  • Place of Presentation: 新横浜プリンスホテル (横浜市)
  • Year and Date: 2014-08-06 – 2014-08-08
• [Presentation] The role of direct phosphorylation of myosin light chain by Rho-kinase in Ca2+-sensitization of smooth muscle contraction (2014)
  • Author(s): Sei Kobayashi
  • Organizer: 第91回日本生理学会
  • Place of Presentation: 鹿児島大学（鹿児島県）
  • Invited
• [Presentation] Important role of lipid mediators in molecular mechanisms of abnormal vascular contraction (2012)
  • Author(s): S. Kobayashi
  • Organizer: 第89回日本生理学会(招待講演)
  • Place of Presentation: 長野県松本文化会館（松本）
• [Patent(Industrial Property Rights)] 血管攣縮抑制剤 (2014)
  • Inventor(s): 小林 誠、高柿了 大、岸 博子、張 影、加治屋勝子、 渡邉和晃
  • Industrial Property Rights Holder: 国立大学法人山 口大学、株式会 社ラフィーネイン ターナショナル
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-10-14
• [Patent(Industrial Property Rights)] 血管攣縮抑制剤 (2014)
  • Inventor(s): 高松日出子、小 林誠、宮成健司、 岸 博子、他3名
  • Industrial Property Rights Holder: 株式会社タカマ
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-09-10
• [Patent(Industrial Property Rights)] 血管弛緩作用を有するペプチド及び血管弛緩剤 (2013)
  • Inventor(s): 小林誠、木村友彦、岸博子、加治屋勝子、高田雄一、白土絵里
  • Industrial Property Rights Holder: 山口大学、林兼産業株式会社
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-09-09