| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
A homeobox transcription factor, Nanog, is one of the genes which support the self-renewal and pluripotency of embryonic stem (ES) cells. Nanog can reprogram human somatic cells to pluripotent stem cells in combination with OCT4, SOX2, and LIN28. Molecular mechanism(s) of the regulation of Nanog expression is important for understanding of reprogramming process. We sought to establish the reporter cell line, which can monitor the somatic cell initialization factor expression for elucidating the molecular mechanism(s) of cell reprogramming. In addition, stem cell pluripotency and differentiation are regulated by the signal transduction pathways involeved in G protein coupled receptors (GPCRs). Therefore, we investigate ligand binding mode of the representative GPCRs, human formylpeptide receptor (hFPR) and formylpeptide receptor-like 1 (hFPRL1) by three-dimensional homology modeling of receptors and ligand docking simulation.
|
