Analysis of cell dynamics using intravital nano-imaging.
| Project/Area Number |
23659116
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Research Collaborator |
FUKUDA Norio 東京慈恵会医科大学, 医学部・細胞生理学講座, 准教授 (30301534)
OTSUKI Iwao 東京慈恵会医科大学, 医学部・細胞生理学講座, 客員教授 (70009992)
TERUI Takako 東京慈恵会医科大学, 医学部・麻酔学講座, 助教 (10366247)
HIGUCHI Hideo 東京大学, 理学部・物理学専攻, 教授 (90165093)
SHIMOZAWA Togo 早稲田大学, 生命理工学部, 助教 (00386608)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | in vivoイメージング / 微小管 / サルコメア / 生体運動・形態形成・細胞間相互作用 / 生体ナノイメージング / 細胞運動・形態形成・細胞間相互作用 / ナノイメージング / in vivo |
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| Outline of Final Research Achievements |
In vivo nano-imaging have the potential to investigated molecular dynamics and mechanism in living mammals with nanometer accuracy. In this study, in the xenograft tumors, the mean velocity of EB1-comets suggests that the velocity of MT tips in vivo is affected by surrounding microenvironment of the cells by using high-speed (100fps) and high-resolution (~20 nm) confocal fluorescent microscope. Likewise, using the same imaging system, in living heart, we found that the working range of sarcomere length (1.90 and 1.68 μm in diastole and systole, respectively) existed on the shorter resting distribution side, and the left ventricular developed pressure was linearly correlated with the sarcomere length change between diastole and systole on the order of 100 nm.
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Report
(5 results)
Research Products
(12 results)
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[Journal Article] Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin-AcGFP in Z-discs2014
Author(s)
Shintani, S., Oyama, K., Kobirumaki, F., Shimozawa, F., Ohki, T., Ishiwata, S., and Fukuda, N.
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Journal Title
J. Gen. Physiol.
Volume: 143
Issue: 4
Pages: 513-524
DOI
Related Report
Peer Reviewed
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[Journal Article] Depressed Frank-Starling mechanism in the left ventricular muscle of the knock-in mouse model of dilated cardiomyopathy with troponin T deletion mutation ΔK2102013
Author(s)
Inoue T, Kobirumaki-Shimozawa F, Kagemoto T, Fujii T, Terui T, Kusakari Y, Hongo K, Morimoto S, Ohtsuki I, Hashimoto K, Fukuda N
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Journal Title
J Mol Cell Cardiol.
Volume: 63
Pages: 69-78
DOI
Related Report
Peer Reviewed
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