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Analysis of cell dynamics using intravital nano-imaging.

Research Project

Project/Area Number 23659116
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General physiology
Research InstitutionJikei University School of Medicine

Principal Investigator

FUYU Kobirumaki  東京慈恵会医科大学, 医学部, 助教 (40548905)

Research Collaborator FUKUDA Norio  東京慈恵会医科大学, 医学部・細胞生理学講座, 准教授 (30301534)
OTSUKI Iwao  東京慈恵会医科大学, 医学部・細胞生理学講座, 客員教授 (70009992)
TERUI Takako  東京慈恵会医科大学, 医学部・麻酔学講座, 助教 (10366247)
HIGUCHI Hideo  東京大学, 理学部・物理学専攻, 教授 (90165093)
SHIMOZAWA Togo  早稲田大学, 生命理工学部, 助教 (00386608)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsin vivoイメージング / 微小管 / サルコメア / 生体運動・形態形成・細胞間相互作用 / 生体ナノイメージング / 細胞運動・形態形成・細胞間相互作用 / ナノイメージング / in vivo
Outline of Final Research Achievements

In vivo nano-imaging have the potential to investigated molecular dynamics and mechanism in living mammals with nanometer accuracy. In this study, in the xenograft tumors, the mean velocity of EB1-comets suggests that the velocity of MT tips in vivo is affected by surrounding microenvironment of the cells by using high-speed (100fps) and high-resolution (~20 nm) confocal fluorescent microscope. Likewise, using the same imaging system, in living heart, we found that the working range of sarcomere length (1.90 and 1.68 μm in diastole and systole, respectively) existed on the shorter resting distribution side, and the left ventricular developed pressure was linearly correlated with the sarcomere length change between diastole and systole on the order of 100 nm.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results) (of which Invited: 2 results)

  • [Journal Article] Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin-AcGFP in Z-discs2014

    • Author(s)
      Shintani, S., Oyama, K., Kobirumaki, F., Shimozawa, F., Ohki, T., Ishiwata, S., and Fukuda, N.
    • Journal Title

      J. Gen. Physiol.

      Volume: 143 Issue: 4 Pages: 513-524

    • DOI

      10.1085/jgp.201311118

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cardiac thin filament regulation and the Frank-Starling mechanism.2014

    • Author(s)
      Kobirumaki-Shimozawa F, Inoue T, Shintani SA, Oyama K, Terui T, Minamisawa S, Ishiwata S, Fukuda N.
    • Journal Title

      The Journal of Physiological Sciences

      Volume: Ahead of print

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Depressed Frank-Starling mechanism in the left ventricular muscle of the knock-in mouse model of dilated cardiomyopathy with troponin T deletion mutation ΔK2102013

    • Author(s)
      Inoue T, Kobirumaki-Shimozawa F, Kagemoto T, Fujii T, Terui T, Kusakari Y, Hongo K, Morimoto S, Ohtsuki I, Hashimoto K, Fukuda N
    • Journal Title

      J Mol Cell Cardiol.

      Volume: 63 Pages: 69-78

    • DOI

      10.1016/j.yjmcc.2013.07.001

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sarcomere imaging by quantum dots for the study of cardiac muscle physiology.2012

    • Author(s)
      Fuyu Kobirumaki-Shimozawa, Kotaro Oyama, Takahiro Serizawa, Akari Mizuno, Tatsuya Kagemoto, Togo Shimozawa, Shin'ichi Ishiwata, Satoshi Kurihara and Norio Fukuda
    • Journal Title

      Journal of Biomedicine and Biotechnology

      Volume: Vol. 2012

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] High-speed live imaging of sarcomere dynamics in the beating mouse heart in vivo2015

    • Author(s)
      小比類巻生, 福田紀男
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ナノスケール高速ライブイメージングによるマウスin vivoサルコメア計測2014

    • Author(s)
      小比類巻生, 大山廣太郎, 下澤東吾, 照井貴子, 南沢享, 石渡信一, 福田紀男
    • Organizer
      第12回ナノ学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-05-22 – 2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Real-time imaging of sarcomere dynamics in the mouse heart in vivo2014

    • Author(s)
      Fuyu Kobirumaki-Shimozawa, Kotaro Oyama, Akari Mizuno, Takako Terui, Togo Shimozawa, Takeshi Ohki, Susumu Minamisawa, Shin'ichi Ishiwata, Norio Fukuda
    • Organizer
      Biophysical Society 58th Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 高速ライブイメージングを用いたin vivo単一サルコメア計測2014

    • Author(s)
      小比類巻生, 大山廣太郎, 広川恵里沙, 下澤東吾, 照井貴子, 南沢享, 石渡信一, 福田紀男
    • Organizer
      第91回日本生物物理学会大会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Research-status Report
  • [Presentation] 生きたマウスの心臓における単一サルコメア長解析2013

    • Author(s)
      小比類巻生, 大山廣太郎, 照井貴子, 水野紅理, 影本達也, 下澤東吾, 石渡信一, 福田紀男
    • Organizer
      第11回ナノ学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] Real-time high-resolution cardiac imaging in vivo2013

    • Author(s)
      小比類巻生, 大山廣太郎, 新谷正嶺, 広川恵里沙, 下澤東吾, 照井貴子, 石渡信一, 福田紀男
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Real-time measurement of sarcomere length in the rodent heart by using α-Actinin-GFP2011

    • Author(s)
      Fuyu Kobirumaki, Takako Terui, Akari Mizuno, Tatsuya Kagemoto, Togo Shimozawa, Shin'ichi Ishiwata, Norio Fukuda
    • Organizer
      日本生物物理学会
    • Place of Presentation
      兵庫県立大学・姫路書写キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] α-Actinin-GFPを用いたアダルト心筋細胞のサルコメア長計測2011

    • Author(s)
      小比類巻 生, 照井 貴子, 水野 紅理, 影本 達也, 下澤 東吾, 石渡 信一, 栗原 敏, 福田 紀男
    • Organizer
      ナノ学会
    • Place of Presentation
      北海道大学 学術交流会館
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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