| Project/Area Number |
23659117
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
KUBO Yoshihiro 生理学研究所, 分子生理研究系, 教授 (80211887)
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| Research Collaborator |
KECELI Batu 生理学研究所, 分子生理研究系, 特別協力研究員 (80645250)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 生理学 / 生体分子 / タンパク質 / ATP / 受容体 / 蛋白質 |
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| Research Abstract |
ATP receptor channel P2X_2 consists of three subunits, but it is known to be activated by binding of two ATP. The aim of this research was to understand how the activation signal by two ATP flows in the trimer. By establishing a method to introduce mutations controlling the number of mutations in the trimer, we introduced 1, 2 or 3 mutations at various amino acid residues in the trimer. The results suggested that the activation signal flows on the two ATP bound subunits down to the linker level and it spreads to three subunits at the ion channel pore level.
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