| Project/Area Number |
23659124
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Kagoshima University |
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Principal Investigator |
UEDA Masahiro 鹿児島大学, 自然科学教育研究支援センター, 技術職員 (00448573)
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| Co-Investigator(Kenkyū-buntansha) |
KISHIDA Shosei 鹿児島大学, 医歯学総合研究科, 教授 (50274064)
IIJIMA Mikio 鹿児島大学, 医歯学総合研究科, 助教 (00305111)
KISHIDA Michiko 鹿児島大学, 医歯学総合研究科, 助教 (40274089)
乾 明夫 鹿児島大学, 医歯(薬)学総合研究科, 教授 (80168418)
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| Co-Investigator(Renkei-kenkyūsha) |
INUI Akio 鹿児島大学, 医歯学総合研究科, 教授 (80168418)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 摂食調節 / デスアシルグレリン / 受容体 |
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| Research Abstract |
Ghrelin is a peptide hormone to regulate appetite and is known to have two forms (acyl ghrelin and des-acyl ghrelin). Ghrelin receptor (GHSR), which recognizes the post-translational acyl-modification, expressed in various tissues. However, ghrelin without lipid-modification does not bind to GHSR and functions antagonistic to acyl ghrelin. These findings suggested the presenceof unknown receptor specific for des-acyl ghrelin. We performed affinity-purification of des-acyl ghrelin-binding protein using pig brain extract. We tried to elute des-acyl ghrelin-associated protein by high salt condition (1M NaCl) but detected no significant condensation of candidate molecules. Next, we performed conventional receptor panning assay using COS7 cells transfected with sub-pooled mouse brain cDNA libraries. We detected several pools as “positive pools” and further selection of procedures for cloning is in process.
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