Identification and characterization of desacyl-ghrelin
Project/Area Number |
23659124
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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Research Institution | Kagoshima University |
Principal Investigator |
UEDA Masahiro 鹿児島大学, 自然科学教育研究支援センター, 技術職員 (00448573)
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Co-Investigator(Kenkyū-buntansha) |
KISHIDA Shosei 鹿児島大学, 医歯学総合研究科, 教授 (50274064)
IIJIMA Mikio 鹿児島大学, 医歯学総合研究科, 助教 (00305111)
KISHIDA Michiko 鹿児島大学, 医歯学総合研究科, 助教 (40274089)
乾 明夫 鹿児島大学, 医歯(薬)学総合研究科, 教授 (80168418)
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Co-Investigator(Renkei-kenkyūsha) |
INUI Akio 鹿児島大学, 医歯学総合研究科, 教授 (80168418)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 摂食調節 / デスアシルグレリン / 受容体 |
Research Abstract |
Ghrelin is a peptide hormone to regulate appetite and is known to have two forms (acyl ghrelin and des-acyl ghrelin). Ghrelin receptor (GHSR), which recognizes the post-translational acyl-modification, expressed in various tissues. However, ghrelin without lipid-modification does not bind to GHSR and functions antagonistic to acyl ghrelin. These findings suggested the presenceof unknown receptor specific for des-acyl ghrelin. We performed affinity-purification of des-acyl ghrelin-binding protein using pig brain extract. We tried to elute des-acyl ghrelin-associated protein by high salt condition (1M NaCl) but detected no significant condensation of candidate molecules. Next, we performed conventional receptor panning assay using COS7 cells transfected with sub-pooled mouse brain cDNA libraries. We detected several pools as “positive pools” and further selection of procedures for cloning is in process.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis2013
Author(s)
Kibe T, Fuchigami T, Kishida M, Iijima M, Ishihata K, Hijioka H, Miyawaki A, Semba I, Nakamura N, Kiyono T, Kishida S
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Journal Title
Oral Surg Oral Med Oral Pathol Oral Radiol
Volume: 115(6)
Issue: 6
Pages: 780-8
DOI
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Peer Reviewed
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[Journal Article] ntelligence quotient and cognitive functions in severerestricting-type anorexia nervosa before and after weight gain.2012
Author(s)
Koyama KI, Asakawa A, Nakahara T, Amitani H, Amitani M, Saito M, Taruno Y, Zoshiki T, Cheng KC, Yasuhara D, Inui A
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Journal Title
Nutrition.
Volume: 28(11-12)
Issue: 11-12
Pages: 1132-6
DOI
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[Journal Article] Effects of supplementation on food intake, body weight and hepatic metabolites in the citrin/mitochondrial glycerol-3-phosphate dehydrogenase double-knockout mouse model of human citrin deficiency2012
Author(s)
Saheki T, Inoue K, Ono H, Katsura N, Yokogawa M, Yoshidumi Y, Furuie S, Kuroda E, Ushikai M, Asakawa A, Inui A, Eto K, Kadowaki T, Sinasac DS, Yamamura K, Kobayashi K
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Journal Title
Mol Genet Metab
Volume: 107(3)
Issue: 3
Pages: 322-9
DOI
Related Report
Peer Reviewed
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[Journal Article] Plasma ghrelin isoforms and gastric ghrelin O-acyltransferase expression are influenced by Helicobacter pylori status2012
Author(s)
Ando T, Mizuno S, Ishida T, Kondo Y, Miki I, Yoshida M, Azuma T, Ishikawa T, Takagi T, Yagi N, Kokura S, Naito Y, Yoshikawa T, Asakawa A, Inui A
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Journal Title
Nutrition.
Volume: 28(10)
Issue: 10
Pages: 967-72
DOI
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[Journal Article] Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes2011
Author(s)
Toshiro Kibe, Michiko Kishida, Masayuki Kamino, Mikio Iiima, Lin Clen, Mika Habu, Akihiko Miyawaki, Hiroshi Hijioka, Norifumi Nakamura, Tohru Kiyono, Shosei Kishida
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Journal Title
Oral Science International
Volume: 8(1)
Issue: 1
Pages: 20-28
DOI
NAID
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Peer Reviewed
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[Presentation] Etude de l'expression de la ghreline central,l'anxieteet la depression dans un modele d'anorexie induite par le methotrexate chezle rat2012
Author(s)
Francois M, Takagi K, Tennoune N, Beutheu Youmba S, Bole-Feysot C, Cravezic A, do Rego JC, Coeffier M, Inui A, Dechelotte P, Fetissov SO
Organizer
1er Colloque du GIR-AFDAS-TCA(groupe interdisciplinaire de recherche de l'association francaise pour le developpement des approaches specialisees des troubles du comportement alimentaire)
Place of Presentation
Paris
Year and Date
2012-02-09
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