Elucidation of molecular mechanism of cell damage by cigarette smoke extract and development of cigarette smoke detoxification methods
| Project/Area Number |
23659129
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Hokkaido University |
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Principal Investigator |
MIWA Soichi 北海道大学, 大学院・医学研究科, 教授 (40157706)
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| Co-Investigator(Kenkyū-buntansha) |
HORINOUCHI Takahiro 北海道大学, 大学院・医学研究科, 講師 (20307771)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タバコ煙 / メチルビニルケトン / シクロペンテノン / 細胞傷害 / PKC / 喫煙 / タバコ煙水抽出物 / カルボニル化合物 / マクロファージ / 細胞分化 / 動脈硬化 / タバコ煙水抽出液 / プロテインキナーゼC / NADPHオキシダーゼ / 活性酸素種 / 薬理学 |
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| Research Abstract |
Cigarette smoking is one of the major risks for atherosclerosis. We have identified several carbonyl compounds responsible for cell injury from cigarette main stream. These chemicals activated PKC and caused cell membrane damages and apoptosis in intracellular Ca2+-dependent manner in rat glioma C6 cells.. Furthermore, these compounds also caused intracellular Ca2+-dependent cell injury in blood vessel cells (epithelial cells and smooth muscle cells) and blood cells, and promoted differentiation of monocytes into macrophages.
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Report
(3 results)
Research Products
(36 results)
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[Journal Article] Different binding property of STIM1 and its novel splice variant STIM1L to Orai1, TRPC3, and TRPC6 channels2012
Author(s)
Horinouchi T, Higashi T, Higa T, Terada K, Mai Y, Aoyagi H, Hatate C, Nepal P, Harada T, Miwa S
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Journal Title
Biochem Biophys Res Commun
Volume: 428
Pages: 252-258
Related Report
Peer Reviewed
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[Journal Article] Nicotine-and tar-free cigarette smoke induces cell damage through reactive oxugen species newly generated by PKC-dependent activation of NADPH oxidase2012
Author(s)
Asano H, Horinouchi T, Mai Y, Sawada O, Fujii S, Nishiya T, Minami M, Katayama T, Iwanaga T, Terada K, Miwa S
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Journal Title
J Pharmacol Sci
Volume: 118
Pages: 275-287
Related Report
Peer Reviewed
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