Roles of p40phox in Crohn disease and its application for drug development
Project/Area Number |
23659137
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General pharmacology
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Research Institution | Kobe University |
Principal Investigator |
SAITOU Naoaki 神戸大学, 自然科学系先端融合研究環バイオシグナル研究センター, 教授 (60178499)
|
Co-Investigator(Kenkyū-buntansha) |
UEYAMA Takehiko 神戸大学, 自然科学系先端融合研究環バイオシグナル研究センター, 准教授 (80346254)
楠 正人 三重大学, 医学(系)研究科(研究院), 教授 (50192026)
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Co-Investigator(Renkei-kenkyūsha) |
KUSUNOKI Masato 三重大学, 医学(系)研究科(研究院) (50192026)
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Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | クローン病 / p40phox / 活性酸素 / NADPH / oxidase / 遺伝子異常 / 乳幼児 / オートファジー / モデルマウス / 遺伝子解析 |
Research Abstract |
On the basis of our hypothesis that the etiology of Crohn’s disease is related with that of CGD enteritis due to p40phox gene abnormality, we examined the new cause of Crohn disease and its treatment. We examined the production of reactive oxygen, and p40phox gene mutations in treatment-resistant of the six cases the (refractory) patients with Crohn's disease, but we were not able to discover the CGD enteritis patients due to p40phox gene abnormality.
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Report
(3 results)
Research Products
(50 results)
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[Journal Article] Novel PKCα-mediated phosphorylation site(s) on cofilin and their potential role in terminating histamine release2012
Author(s)
Sakuma, M., Shirai, Y., Yoshino, K-I., Kuramasu, M., Nakamura, T., Yanagita, T., Mizuno, K., Hide, I., Nakata, Y., Saito, N.
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Journal Title
Mol Biol Cell
Volume: 23(18)
Pages: 3707-3721
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[Journal Article] Establishment of a Novel Fluorescence-Based Method to Evaluate Chaperone-Mediated Autophagy in a Single Neuron.2012
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Seki, T., Ken-ich Yoshino, K., Tanaka, S., Dohi, E., Onji, T., Yamamoto, K., Hide, I., Paulson, H.L., Saito, N., Sakai, N
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Journal Title
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[Journal Article] c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase αby phosphorylation at Tyr2182012
Author(s)
Matsubara, T., Ikeda, M., Kiso, Y., Sakuma, M., Yoshino, K-I., Sakane, F., Merida, I., Saito, N. and Shirai, Y
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 5507-5517
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[Journal Article] Novel PKCalphamediated phosphorylation site(s) on cofilin and their potential role in2012
Author(s)
Sakuma, M., Shirai, Y., Yoshino, K^I., Kuramasu,M., Nakamura, T., Yanagita, T., Mizuno, K.,Hide, I., Nakata, Y., Saito, N.
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Journal Title
Mol Biol. Cell
Volume: 23
Pages: 3707-3721
Related Report
Peer Reviewed
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[Journal Article] Establishment of a Novel Fluorescence-Based Method to Evaluate Chaperone-Mediated Autophagy in a Single Neuron.2012
Author(s)
Seki, T., Yoshino, K., Tanaka, S., Dohi, E., Onji, T., Yamamoto, K., Hide, I., Paulson, H.L., Saito, N., Sakai, N
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Cooperation of p40phox with p47phox for Nox2 activation during FcγR-mediated phagocytosis --Mechanism for acquisition of p40phox PI(3)P binding--2011
Author(s)
Ueyama, T., Nakakita, J., Kobayashi, T., Kobayashi, T., Son, J-h., Sakaguchi, H., Leto, T., and Saito, N
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Journal Title
J. Biol. Chem
Volume: 286
Pages: 40693-40705
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[Journal Article] Elucidation of the Molecular Mechanism and Exploration of Novel Therapeutics for Spinocerebellar Ataxia Caused by Mutant Protein Kinase Cγ2011
Author(s)
Seki, T., Adachi, N., Abe-Seki, N., Shimahara, T., Takahashi, H., Yamamoto, K., Saito, N. and Sakai, N
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Journal Title
J Pharmacol Sci
Volume: 116
Pages: 239-247
NAID
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[Journal Article] Streptococcus mutans diacylglycerol kinase homologue:a potential target for anti-caries chemotherapy2011
Author(s)
Shibata, Y., Kawada-Matsuo, M., Shirai, Y., Saito, N.,Li. D., and Yamashita, Y
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Journal Title
J. Med. Microbiol
Volume: 60
Pages: 625-630
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[Journal Article] Direct binding of RalA to PKCη and its crucial role in morphological change during keratinocytes differentiation2011
Author(s)
Shirai, Y., Morioka, S., Sakuma, M., Yoshino, K., Otsuji, C., Sakai, N., Kashiwagi, K., Chida, K., Shirakawa, R., Horiuchi, H., Nishigori, C., Ueyama, T., Saito, N
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Journal Title
Mol. Biol. Cell
Volume: 22
Pages: 1340-1352
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[Journal Article] Nuclear Choline Acetyltransferase Activates Transcription of a High-affinity Choline Transporter2011
Author(s)
Matsuo, A., Bellier, J-P, Nishimura, M., Yasuhara, O., Saito, N. and Kimura, H
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Journal Title
J. Biol. Chem
Volume: 286
Pages: 5836-5845
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[Journal Article] Establishment of a novel fluorescence-based method to evaluate chaperone-mediate autophagy in a singlz neuron2011
Author(s)
Seki, T., Ki Y., Tanaka, S., Dohi, E., Onji, T., Yamamoto, K., Seki T, Yoshino KI, Tanaka S, Dohi E, Onji T, Yamamoto K, Hide, I., Sakai, N. et al.
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Journal Title
PLoS ONE
Volume: 7
Issue: 2
Pages: e31232-e31232
DOI
Related Report
Peer Reviewed
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[Journal Article] c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase α by phosphorylation at Tyr2182011
Author(s)
Matsubara, M., Ikeda, M., Kiso, Y., Sakuma, M., Sakane, F., Merida, I, Saito, N., Shirai, Y.
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Journal Title
J.Biol.Chem
Volume: 287
Issue: 8
Pages: 5507-5517
DOI
Related Report
Peer Reviewed
-
[Journal Article] Cooperation of p40phox with p47phox for Nox2 activation during Fc{lower case gamma}R-mediated phagocytosis --Mechanism for acquisition of p40phox PI(3)P binding--"2011
Author(s)
Ueyama, T., Nakakita, J., Kobayashi, T., Kobayashi, T., -hyun Son, J-h., Sakaguchi, H., Leto, T., and Saito, N
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Journal Title
J. Biol. Chem.
Volume: 286
Pages: 40693-40705
Related Report
Peer Reviewed
-
-
[Journal Article] Elucidation of the Molecular Mechanism and Exploration of Novel Therapeutics for Spinocerebellar Ataxia Caused by Mutant Protein Kinase Cγ.2011
Author(s)
Seki, T., Adachi, N., Abe-Seki, N., Shimahara, T., Takahashi, H., Yamamoto, K., Saito, N. and Sakai, N.
-
Journal Title
J Pharmacol Sci
Volume: 116
Pages: 239-247
NAID
Related Report
Peer Reviewed
-
[Journal Article] Streptococcus mutans diacylglycerol kinase homologue:a potential target for anti-caries chemotherapy.2011
Author(s)
Shibata, Y., Kawada-Matsuo, M., Shirai, Y., Saito, N., Li. D., and Yamashita, Y.
-
Journal Title
J. Med. Microbiol.
Volume: 60
Pages: 625-630
Related Report
Peer Reviewed
-
[Journal Article] Direct binding of RalA to PKC and its crucial role in morphological change during keratinocytes differentiation.2011
Author(s)
Shirai, Y., Morioka, S., Sakuma, M., Yoshino, K., Otsuji, C., Sakai, N., Kashiwagi, K., Chida, K., Shirakawa, R., Horiuchi, H., Nishigori, C., Ueyama, T., Saito, N.
-
Journal Title
Mol. Biol. Cell
Volume: 22
Pages: 1340-1352
Related Report
Peer Reviewed
-
[Journal Article] Nuclear Choline Acetyltransferase Activates Transcription of a High-affinity Choline Transporter.2011
Author(s)
Matsuo, A., Bellier, J-P, Nishimura, M., Yasuhara, O., Saito, N. and Kimura, H.
-
Journal Title
J. Biol. Chem.
Volume: 286
Pages: 5836-5845
Related Report
Peer Reviewed
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[Journal Article] Increased seizure susceptibility in a mouse with diacylglycerol kinase β2 deficiency
Author(s)
Ishisaka, M., Tsuruma, K., Shimazawa, M., Shirai, Y.,Saito, N., and Hara, H
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Journal Title
Related Report
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[Presentation] Glial functions of Rho-family small GTPases during cerebellar development and after CNS injury2012
Author(s)
Ueyama,T., Kondoh T., Kohta M., Kohmura,E., Hayashibe,M., Nakamura,T., Sakahara,M., Kassai,H., Nakao,K., Aiba,A. and Saito,N.
Organizer
14th International congress of histochemistry and cytochemistry
Place of Presentation
京都(ポスター・英語)
Related Report
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[Presentation] Involvment of PKCgamma in neurodegenerative disease2012
Author(s)
Saito, N., Takahashi, H., Shirafuji, T., Adachi, N., Danno, S., Ueyama, T., Seki, T. and Sakai,N
Organizer
14th International congress of histochemistry and cytochemistry
Place of Presentation
京都(ポスター・英語)
Related Report
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[Presentation] Role of PKCα-mediated cofilin phosphorylation in the degranulation from RBL-2H3 mast cells2012
Author(s)
Sakuma, M., Shirai, Y., Yoshino, K., Kuramasu, M., Nakamura, T., Mizuno, K., Hide,I., Nakata,Y.and Saito,N
Organizer
22th IUBMB & 37th FEBS
Place of Presentation
スペイン(ポスター発表・英語)
Related Report
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[Presentation] Glial functions of Rho-family small GTPases during cerebellar development and after CNS injury2012
Author(s)
Ueyama,T., Kondoh T., Kohta M., Kohmura,E., Hayashibe,M., Nakamura,T.,
Organizer
14th International congress of histochemistry and cytochemistry
Place of Presentation
京都
Related Report
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[Presentation] Involvment of PKCgamma in neurodegenerative disease2012
Author(s)
Saito, N., Takahashi, H., Shirafuji, T., Adachi, N., Danno, S., Ueyama, T.,
Organizer
14th International congress of histochemistry and cytochemistry
Place of Presentation
京都
Related Report
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[Presentation] le of PKCalpha-mediated cofilin phosphorylation in the degranulation from RBL-2H3 mast cells.2012
Author(s)
Sakuma, M., Shirai, Y., Yoshino, K., Kuramasu, M., Nakamura, T., Mizuno, K.,
Organizer
22th IUBMB & 37th FEBS
Place of Presentation
スペイン
Related Report
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[Presentation] Novel phosphorylation site(s) of cofilin by PKCα and its negative role in the degranulation from RBL-2H3 mast cells2011
Author(s)
Sakuma,M., Shirai,Y., Yoshino K., Kuramasu,M., Nakamura,T., Mizuno,K., Hide,I., Nakata,Y. and Saito,N
Organizer
he American society for cell biology
Place of Presentation
デンバー(ポスター発表・英語)
Related Report
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[Presentation] Novel phosphorylation site(s) of cofilin by PKCalpha and its negative role in the degranulation from RBL-2H3 mast cells.2011
Author(s)
Sakuma,M., Shirai,Y., Yoshino K., Kuramasu, M., Nakamura,T., Mizuno,K., Hide,I., Nakata,Y. and Saito,N.
Organizer
The American society for cell biology.
Place of Presentation
Denver, USA
Related Report
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