| Project/Area Number |
23659141
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Juntendo University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NEMOTO Naoto 埼玉大学, 理工学研究科, 准教授 (60509727)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KASHIYAMA Taku 順天堂大学, 医学部, 助教 (90338343)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 神経科学 / プロテオーム / 組換え抗体 / 薬理学 |
|---|
| Research Abstract |
Fluorophore-assisted light inactivation (FALI) in combination with a cell-based assay is a uniquemethod for functional proteomic screen to search for regulators of a specific cellular function. One of the major problems with the method is the construction of antibody libraries that contain high-affinity binders to cell surface membrane proteins as potential targets. To overcome barriers to efficiency, we used a cell-free antibody display method. We tried to optimize the in vitro expression of the recombinant antibody that is linked to its genotype and the neuregulin-erbB model system for pilot-scale screening ofpotential regulators for neuron-glia interaction
|
