Disease models using gene expression system in Bombyx mori

• Project/Area Number: 23659144
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Hokkaido University
• Principal Investigator: HATAKEYAMA Shigetsugu 北海道大学, 大学院・医学研究科, 教授 (70294973)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ゲノム医化学 / 遺伝学 / 遺伝子 / 昆虫 / タンパク質 / ウイルス

Research Abstract

We tried to establish gene expression system in Bombyx mori in order to use Bombyx as a model for analyzing human disease. We used specific Bombyx strains which can be infected by AcNPV. After AcNPV containing a target gene (GFP gene) was infected in this specific Bombyx strains, GFP was transiently expressed in Bombyx.

Research Products

• [Journal Article] Activation of double-stranded RNA-activated protein kinase (PKR) by interferon-stimulated gene 15 (ISG15) modification down-regulates protein translation (2013)
  • Author(s): Okumura, F., Okumura, AJ., Uematsu, K., Hatakeyama, S., Zhang, DE., Kamura, T
  • Journal Title: J Biol Chem Volume: 288(4) Issue: 4 Pages: 2839-47
  • DOI: 10.1074/jbc.m112.401851
  • Peer Reviewed
• [Journal Article] 14-3-3 proteins sequester a pool of soluble TRIM32 ubiquitin ligase to repress autoubiquitination and cytoplasmic body formation. (2013)
  • Author(s): Ichimura, T., Taoka, M., Shoji, I., Kato, H., Sato, T., Hatakeyama, S., Isobe, T. and Hachiya, N.
  • Journal Title: J. Cell Sci. Volume: in press
  • Peer Reviewed
• [Journal Article] Ubiquitin-mediated regulation of JAK-STAT signaling in embryonic stem cells (2012)
  • Author(s): Hatakeyama, S
  • Journal Title: JAK-STAT Volume: 1 Pages: 168-175
  • Peer Reviewed
• [Journal Article] TRIM45 negatively regulates NF-kB-mediated transcription and suppresses cell prolifration. (2012)
  • Author(s): Shibata, M., Sato, T., Nukiwa, R., Ariga, T. and Hatakeyama, S.
  • Journal Title: Biophys. Res. Commun. Volume: 423 Issue: 1 Pages: 104-109
  • DOI: 10.1016/j.bbrc.2012.05.090
  • Peer Reviewed
• [Journal Article] TRIM59 interacts with ECSIT and negatively regulates NF-kB and IRF-3/7-mediated signal pathway. (2012)
  • Author(s): Kondo, T., Watanabe, M. and Hatakeyama, S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 422 Issue: 3 Pages: 501-507
  • DOI: 10.1016/j.bbrc.2012.05.028
  • Peer Reviewed
• [Journal Article] Ymer acts as a multufunctional regulator in NF-kB and Fas signaling pathways (2012)
  • Author(s): Tsukiyama, T., et al
  • Journal Title: Mol.Med. Volume: (in press) Issue: 4 Pages: 587-597
  • DOI: 10.2119/molmed.2011.00435
  • Peer Reviewed
• [Journal Article] TRIM6 interacts with c-Myc and maintains pluripotency of mouse embryonal stem cells (2012)
  • Author(s): Sato T, Okumura F, Ariga T, Hatakeyama S
  • Journal Title: J Cell Sci Volume: (in press) Pages: 1544-1555
  • DOI: 10.1242/jcs.095273
  • Peer Reviewed
• [Journal Article] TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and indices neuritogenesis (2012)
  • Author(s): Yaguchi, H., Okumura, F., Takahashi, H., Kano, T., Kameda, H., Uchigashima, M., Tanaka, S., Watanabe, M., Sasaki, H. and Hatakeyama, S.
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 15 Pages: 12050-12059
  • DOI: 10.1074/jbc.m111.307678
  • Peer Reviewed
• [Journal Article] TRIM6 interacts with Myc and maintains the pluripotency of mouse embryonal stem cells (2012)
  • Author(s): Sato, T., Okumura, F., Ariga, T. and Hatakeyama, S.
  • Journal Title: J. Cell Sci. Volume: 125 Issue: 20 Pages: 1544-1555
  • DOI: 10.1242/jcs.088799
  • Peer Reviewed
• [Journal Article] TRIM32 promotes retinoic acid receptor alpha-mediated differentiation in human promyelogenous leukemic cell line HL60 (2012)
  • Author(s): Sato, T., Okumura, F., Iguchi, A., Ariga, T. and Hatakeyama, S.
  • Journal Title: Biochem. Biophys. Res.Commun. Volume: 417 Issue: 4 Pages: 594-600
  • DOI: 10.1016/j.bbamcr.2011.12.012
  • Peer Reviewed
• [Journal Article] Ubiquitin-mediated regulation of JAK-STAT signaling in embryonic stem cells. (2012)
  • Author(s): Hatakeyama, S.
  • Journal Title: JAK-STAT Volume: 1 Pages: 168-175
  • Peer Reviewed
• [Journal Article] TRIM proteins and cancer (2011)
  • Author(s): Hatakeyama, S.
  • Journal Title: Nature Rev. Cancer Volume: 11 Issue: 11 Pages: 792-804
  • DOI: 10.1038/nrc3139
  • Peer Reviewed
• [Journal Article] TRIM8 regulates Nanog via Hsp90 beta-mediated nuclear translocation of STAT3 in embryonic stem cells (2011)
  • Author(s): Okumura, F., Okumura, A.J., Matsumoto, M., Nakayama, K.I. and Hatakeyama, S.
  • Journal Title: BBA-Mol. Cell Res. Volume: 1813 Issue: 10 Pages: 1784-1792
  • DOI: 10.1016/j.bbamcr.2011.05.013
  • Peer Reviewed
• [Journal Article] TRIM29 negativelyregulates p53 via inhibition of Tip60 (2011)
  • Author(s): Sho, T., Tsukiyama, T., Sato, T., Kondo, T., Cheng, J., Saku, T., Asaka, M. and Hatakeyama, S.
  • Journal Title: BBA-Mol. Cell Res. Volume: 1813 Issue: 6 Pages: 1245-1253
  • DOI: 10.1016/j.bbamcr.2011.03.018
  • Peer Reviewed
• [Journal Article] MDA-9/syntenin interacts with ubiquitin via a novel ubiquitin-binding motif (2011)
  • Author(s): Okumura, F., et al
  • Journal Title: Mol.Cell.Biochem. Volume: 352 Issue: 1-2 Pages: 163-172
  • DOI: 10.1007/s11010-011-0750-4
  • Peer Reviewed
• [Journal Article] TRM40 promotes neddylation of IKK gamma and is downregulated in gastrointestinal cancers (2011)
  • Author(s): Noguchi, K., Okumura, F., Takahashi, N., Kataoka, A., Kamiyama, T., Todo, S. and Hatakeyama, S.
  • Journal Title: Carcinogenesis Volume: 32 Issue: 7 Pages: 995-1004
  • DOI: 10.1093/carcin/bgr068
  • Peer Reviewed
• [Journal Article] 14-3-3 proteins sequester a pool of soluble TRIM32 ubiquitin ligase to repress autoubiquitination and cytoplasmic body formation
  • Author(s): Ichimura, T., Taoka, M., Shoji, I., Kato, H., ato, T., Hatakeyama, S., Isobe, T. and Hachiya, N
  • Journal Title: J. Cell Sci Volume: (in press)
  • Peer Reviewed
• [Presentation] TRIM proteins and cancers (2012)
  • Author(s): Hatakeyama, S
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌, 札幌
  • Year and Date: 2012-09-19
• [Presentation] RNF43はNEDL1と結合し,p53依存性の転写を調節する (2012)
  • Author(s): 築山忠維,畠山鎮次
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌,札幌
  • Year and Date: 2012-09-19
• [Presentation] 癌化を制御するTRIMファミリーユビキチンリガーゼ (2012)
  • Author(s): 畠山鎮次
  • Organizer: 日本実験動物技術者協会北海道支部第59回特別講演
  • Place of Presentation: 北海道大学,札幌
  • Year and Date: 2012-04-26
• [Presentation] Med26 は Little elongation complex をリクルート することで small nuclearRNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚, 佐藤智信, Conaway, J.W., Conaway, R.C.,山鎮次
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡,福岡
• [Presentation] double-stranded RNA-activated protein kinase (PKR) は interferonstimulated gene 15 (ISG15)修飾により活性化され,キャップ構造依存性のタンパク質翻訳を抑制する (2012)
  • Author(s): 奥村文彦,畠山鎮次,ドンガーザン,嘉村巧
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡,福岡
• [Presentation] Med26はLittle elongation complexをリクルートすることで small nuclearRNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚, Joan W. Conaway,Ronald C.Conaway,山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡,福岡
• [Presentation] TRIM29によるTRRAP/Tip60複合体の機能制御とがん化との関わり (2012)
  • Author(s): 桝田安志,高橋秀尚,畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡,福岡
• [Presentation] TRIMタンパク質の多様性と機能 (2012)
  • Author(s): 畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡,福岡
• [Presentation] RNF43 は canonical/noncanonical Wnt シグナルの両方を抑制する (2012)
  • Author(s): Tadasuke Tsukiyama, Akimasa Fukui, Sayuri Terai, Keisuke Shinada, Shigetsugu Hatakeyama
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡,福岡
• [Presentation] Human Mediator subunit Med26 plays a role in recruitment of "Little elongation complex" to small nuclear RNA genes (2012)
  • Author(s): Hidehisa Takahashi, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場 , マリンメッセ福岡
• [Presentation] TRIM proteins in inflammation and carcinogenesis (2012)
  • Author(s): Hatakeyama, S
  • Organizer: 2nd international symposium: Infection-related canbcers
  • Place of Presentation: (Institute for Genetic Medicine, Hokkaido University, Research Center for Infection-associated Cancer (RCIAC), Sapporo
• [Presentation] 癌化を制御するTRIMファミリーユビキチンリガーゼ (2012)
  • Author(s): 畠山鎮次
  • Organizer: 第21回泌尿器科分子・細胞研究会
  • Place of Presentation: 北海道大学医学部学友会館「フラテ」,札幌
• [Presentation] Ymer functions as a multifunctional regulator in NF-kB and Fas signaling pathways (2012)
  • Author(s): Tsukiyama T., Tsukiyama M M., bohgaki M., Tanaka S., Hatakeyama, S
  • Organizer: the 41st meeting of the Australasian Society for immunology2011
  • Place of Presentation: Adelaide Convention Center, Adelaide
• [Presentation] ダイオキシン受容体内因性リガンドによる前立腺がん細胞におけるアンドロゲンレセプター発言低下と増殖抑制 (2012)
  • Author(s): 丸山覚,築山忠維,河津隆文,佐藤択矢,千葉博基,安部崇重,佐澤陽,篠原信雄,畠山鎮次,野々村克也
  • Organizer: 第21回泌尿器科分子・細胞研究会
  • Place of Presentation: 北海道大学医学部学友会館「フラテ」,札幌
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚,佐藤智信,Conaway, J.W., Conaway, R.C.,畠山鎮次
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] double-stranded RNA-activated protein kinase (PKR)はinterferonstimulated gene 15 (ISG15)修飾により活性化され、キャップ構造依存性のタンパク質翻訳を抑制する (2012)
  • Author(s): 奥村文彦,畠山鎮次,ドンガー ザン,嘉村巧
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡第35回日本分子生福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 髙橋秀尚、Joan W. Conaway、Ronald C. Conaway、畠山 鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] TRIM29によるTRRAP/Tip60複合体の機能制御とがん化との関わり (2012)
  • Author(s): 桝田安志、髙橋秀尚、畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] TRIMタンパク質の多様性と機能 (2012)
  • Author(s): 畠山 鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡第35福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] RNF43はcanonical/noncanonical Wntシグナルの両方を抑制する (2012)
  • Author(s): Tadasuke Tsukiyama, Akimasa Fukui, Sayuri Terai, Keisuke Shinada, Shigetsugu Hatakeyama
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] Human Mediator subunit Med26 plays a role in recruitment of "Little elongation complex" to small nuclear RNA genes (2012)
  • Author(s): Hidehisa Takahashi, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] 癌化を制御するTRIMファミリーユビキチンリガーゼ (2012)
  • Author(s): 畠山 鎮次
  • Organizer: 第21回泌尿器科分子・細胞研究会(招待講演)
  • Place of Presentation: 北海道大学（札幌市）
• [Presentation] ダイオキシン受容体内因性リガンドによる前立腺がん細胞におけるアンドロゲンレセプター発言低下と増殖抑制 (2012)
  • Author(s): 丸山 覚、築山 忠維、河津 隆文、佐藤 択矢、千葉 博基、安部 崇重、佐澤 陽、篠原 信雄、 畠山 鎮次、野々村 克也
  • Organizer: 第21回泌尿器科分子・細胞研究会
  • Place of Presentation: 北海道大学（札幌市）
• [Presentation] Role of apolipoprotein B100 and oxidized low-density lipoprotein in anti beta2 glycoprotein I induced tissue factor expression on monocytes (2011)
  • Author(s): Otomo K, Atsumi T, Fujieda Y, Nakagawa H, Amengual O, Kato M, Horita T, Tasuda S, Matsumoto M, Hatakeyama, S., Koike T
  • Organizer: The 75th annual meeting of the American College of Rheumatology
  • Place of Presentation: Cicago, Illinois
• [Presentation] TRIMファミリー分子によるPPARγシグナルの制御 (2011)
  • Author(s): 渡部昌,畠山鎮次
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館,京都
• [Presentation] TRIMファミリー分子によるPPARγシグナルの制御 (2011)
  • Author(s): 渡部 昌、畠山 鎮次
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] ランチタイム教育講演2 「免疫と癌を制御するTRIM型ユビキチンリガーゼ」
  • Author(s): 畠山 鎮次
  • Organizer: 第40回日本臨床免疫学会総会
  • Place of Presentation: 京王プラザホテル札幌（札幌）
  • Invited
• [Presentation] シンポジウム：オートファジー・プロテオステイシスとがん「TRIM proteins and cancer」
  • Author(s): Hatakeyama, S
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（札幌）
• [Presentation] RNF43はNEDL1と結合し、ｐ53依存性の転写を調節する（ポスター）
  • Author(s): 築山忠維、畠山鎮次
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（札幌）
• [Presentation] TRIM proteins in inflammation and carcinogenosis
  • Author(s): Hatakeyama, S.
  • Organizer: 2nd International Symposium INFECTION-ASSOCIATED CANCERS(招待講演)
  • Place of Presentation: The Hokkaido University Conference Hall, Sapporo
• [Presentation] Ymer functions as a multifunctional regulator in NF-kB and Fas signaling pathways.
  • Author(s): Tsukiyama T., Tsukiyama M M., bohgaki M., Tanaka S., Hatakeyama S.
  • Organizer: the 41st meeting of the Australasian Society for immunology 2011
  • Place of Presentation: Adelaide Convention Center, Adelaide
• [Presentation] Role of apolipoprotein B100 and oxidized low-density lipoprotein in anti beta2 glycoprotein I induced tissue factor expression on monocytes.pathways.
  • Author(s): Otomo K, Atsumi T, Fujieda Y, Nakagawa H, Amengual O, Kato M, Horita T, Tasuda S, Matsumoto M, Hatakeyama S, Koike T.
  • Organizer: The 75th annual meeting of the American College of Rheumatology
  • Place of Presentation: McCormick Place, Chicago, Illinois
• [Book] 標準生化学 (2012)
  • Author(s): 畠山鎮次
  • Total Pages: 351
  • Publisher: 医学書院
• [Book] 「標準生化学」 (2012)
  • Author(s): 藤田道也
  • Total Pages: 351
  • Publisher: 医学書院
• [Book] 複雑化するユビキチンリガーゼの機能と多様性 (2011)
  • Author(s): 畠山鎮次
  • Publisher: 羊土社
• [Book] PSA and Prostate Cancer (2011)
  • Author(s): Maruyama, S. , Nonomura, K, Hatakeyama, S
  • Publisher: Nova Science Publishers
• [Book] タンパク質分解系による生体制御「複雑化するユビキチンリガーゼの機能と多様性」 (2011)
  • Author(s): 畠山 鎮次、渡部 昌
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.hucc.hokudai.ac.jp/~d20505/index.html
• [Patent(Industrial Property Rights)] 前立腺基底細胞の検出方法 (2012)
  • Inventor(s): 畠山鎮次,田中伸哉
  • Industrial Property Rights Holder: 畠山鎮次,田中伸哉
  • Industrial Property Number: 2012-277980
  • Filing Date: 2012-12-20
• [Patent(Industrial Property Rights)] 前立腺基底細胞の検出方法 (2012)
  • Inventor(s): 畠山鎮次、田中伸哉
  • Industrial Property Rights Holder: 畠山鎮次、田中伸哉
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-277980
  • Filing Date: 2012-12-20