| Project/Area Number |
23659146
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Tokyo University of Pharmacy and Life Science |
|---|
Principal Investigator |
ITOH Fumiko 東京薬科大学, 生命科学部, 准教授 (70502582)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | iPS細胞 / 遺伝子改変マウス / TGF-ss / インヒビター / CAG-Cre / TGF-β / inhibitor / MET |
|---|
| Research Abstract |
It is required for the improvement and process of establishment efficiency induced pluripotent stem (iPS) cells. Reprogramming of the cells involves an intermediate mesenchymal-epithelial transition (MET)-like process that is also regulated by TGF ss In this study, we have tried to find out the molecularmechanisms in MET process regulated by TGF-ss. We used mouse embryonic fibroblast (MEF) cells from TGF-b receptors conditional knockout mice crossing with CAG-Cre Tg mice. Then we generated iPS cellsfrom the MEF cells by lentivirus vectors which encode Nakayama factors under the control of doxycycline dependent expression system. These iPS cells showed clonal variety and required longer passage for stabilization.
|
