Gene regulation by amino acids

• Project/Area Number: 23659147
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Chiba University
• Principal Investigator: TAKIGUCHI Masaki 千葉大学, 大学院・医学研究院, 教授 (40179578)
• Co-Investigator(Kenkyū-buntansha): 松本 絵里子 千葉大学, 医学(系)研究科(研究院), 技術職員 (20422256)
• Co-Investigator(Renkei-kenkyūsha): MATSUMOTO Eriko 千葉大学, 大学院・医学研究院, 技術職員 (20422256)
• Research Collaborator: IWASE Katsuro 千葉大学, 大学院・医学研究院, 講師 (80322030) ; OOHIRA Ayano 千葉大学, 大学院・医学研究院, 技術補佐員 ; ARITA Emiko 千葉大学, 大学院・医学研究院, 技術補佐員 ; TAIRA Akiko 千葉大学, 大学院・医学研究院, 博士課程大学院生
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生体分子医学 / 生物時計 / 時間生物学 / 糖新生 / オルニチンサイクル / グルカゴン / 日周リズム / 概日リズム / 遺伝子発現 / 代謝調節

Research Abstract

In the liver of nocturnal mice fed on the standard diet, the gluconeogenic pathway, which synthesizes glucose from amino acids, is activated during the resting-fasting phase i.e. the light period. Here, we showed that in the liver of mice fed on high protein diets, compared to the standard diet, mRNA levels of gluconeogenic enzymes were increased during the acting-feeding phase i.e. the dark period. While corticosterone levels in the plasma showed no apparent change, glucagon increased prominently during the feeding phase of high protein diets, and was regarded as a candidate for the humoral factor that mediates the activation of gluconeogenic enzyme genes in response to dietary amino acids.

Research Products

• [Journal Article] Autologous antibody to src-homology 3-domain GRB2-like 1 specifically increases in the sera of patients with low-grade gliomas (2012)
  • Author(s): 5. Matsutani T, Hiwasa T, Takiguchi M, Oide T, Kunimatsu M, Saeki N, Iwadate Y
  • Journal Title: J Exp Clin Cancer Res Volume: 31 Issue: 1 Pages: 31-85
  • DOI: 10.1186/1756-9966-31-85
  • Peer Reviewed
• [Journal Article] Functional similarity of anticancer drugs by MTT Bioassay (2011)
  • Author(s): Hiwasa, T., Utsumi, T., Yasuraoka, M., Hanamura, N., Shimada, H., Nakajima, H., Kitagawa, M., Iwadate, Y., Goto, K.I., Takeda, A., Ohtsuka, K., Ariga, H., and Takiguchi, M.
  • Journal Title: J. Cancer Sci. Therapy Volume: 3 Pages: 250-255
  • Peer Reviewed
• [Journal Article] Identification of a novel SEREX antigen family, ECSA, in esophageal squamous cell carcinoma (2011)
  • Author(s): Kagaya A, Shimada H, Shiratori T, et al.(2番目/17人中)
  • Journal Title: Proteome Science Volume: 9 Issue: 1 Pages: 31-31
  • DOI: 10.1186/1477-5956-9-31
  • Peer Reviewed
• [Journal Article] Calpain regulates thymidylate synthase-5-fluoro-dUMP complex levels associated with response to 5-fluorouracil in gastric cells (2011)
  • Author(s): Nabeya, Y., Suzuki, T., Furuya, A., Koide, N., Ohkoshi, M., Takiguchi, M., Ochiai, T., Matsubara, H., and Hiwasa, T.
  • Journal Title: Cancer Sci Volume: 102 Pages: 1509-1515
  • Peer Reviewed
• [Journal Article] Functional similarity of anticancer drugs by MTT bioassay (2011)
  • Author(s): Hiwasa T, Iwadate Y, et al.
  • Journal Title: J Cancer Science & Therapy Volume: 3 Issue: 10 Pages: 250-255
  • DOI: 10.4172/1948-5956.1000099
  • Peer Reviewed
• [Journal Article] Calpain regulates thymidylate synthase-5-fluoro-dUMP complex levels associated with response to 5-fluorouracil in gastric cancer cells (2011)
  • Author(s): Nabeya, Y., Suzuki, T., Furuya, A., Koide, N., Ohkoshi, M., Takiguchi, M., Ochiai, T., Matsubara, H., Hiwasa, T.
  • Journal Title: Cancer Science Volume: 102 Issue: 8 Pages: 1509-1515
  • DOI: 10.1111/j.1349-7006.2011.01978.x
  • NAID: 10030242173
  • Peer Reviewed
• [Presentation] アルファライザ法による食道癌SEREX抗原の再評価 (2012)
  • Author(s): 日和佐隆樹(発表者),劉天玲,島田英昭,松原久裕,瀧口正樹
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-16
• [Presentation] 脳梗塞と糖尿病の診断に有用な血清抗体マーカーの同定 (2012)
  • Author(s): 日和佐隆樹(発表者),張暁萌,薦田操,瀧口正樹,森雅裕,鵜沢顕之,武藤真弓,桑原聡,竹本稔,小林一貴,横手幸太郎,青墳章代,和田猛,吉川由利子,片山薫,町田利生,土居洋文,工藤孝,中村利華,冨吉郷,新免奈津子,吉田涼,太田優子,黒田英行
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] 脳梗塞関連ペプチドアレイ法を用いた動脈硬化血清抗体マーカーの探索 (2011)
  • Author(s): 日和佐隆樹(発表者),國松己歳,伊藤舞,小越朱根,張暁萌,町田利生,中村利華,冨吉郷,新免奈津子,吉田涼,太田優子,黒田英行,瀧口正樹
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-24
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/seika1/
• [Patent(Industrial Property Rights)] 整長cDNA 由来両鎖cRNA サブトラクション方法 (2012)
  • Inventor(s): 瀧口正樹,岩瀬克郎,大塚里子,坂尾詠子,宮内修
  • Industrial Property Rights Holder: 瀧口正樹,岩瀬克郎
  • Filing Date: 2012-07-27