Establish of iPS by expression of microRNA

• Project/Area Number: 23659148
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: WATANABE Sumiko 東京大学, 医科学研究所, 特任教授 (60240735)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: miRNA / iPS / 分化 / MEF / RNA / microRNA / 過剰発現 / マウス / レトロウイルス

Research Abstract

miRNA expression pattern of human and mouse iPS was examined by qPCR array followed by bioinformatics, and a list of miRNA, which are expressing highly in iPS, was constructed. Expression vector of these miRNA was made, and they were introduced into MEF, immature iPS, and embryoid body to examine effects of these miRNA for efficiency to establish iPS, immaturity of iPS, and differentiation ability. Several types of expression vector had been constructed, and finally we got a vector expressing miRNA or its antagonized sequence stablyand with high level.

Research Products

• [Journal Article] The early retinal progenitor-expressed gene Sox11 regulates the timing of the differentiation of retinal cells (2013)
  • Author(s): Usui, A. et al.
  • Journal Title: Development Volume: 140 Pages: 740-750
  • Peer Reviewed
• [Journal Article] Selection of RNA aptamers against mouse embryonic stemcells (2012)
  • Author(s): Iwagawa, T., Ohuchi, S., Watanabe, S., Nakamura, Y.
  • Journal Title: Biochimie Volume: 94 Pages: 250-257
  • Peer Reviewed
• [Journal Article] In vitro cell subtype-specific transduction of adeno-associated virus in the mouse and marmoset retinal explant culture (2012)
  • Author(s): Baba, Y., Satoh, S., Otsu, M., Sasaki, E., Okada, T., and Watanabe, S.
  • Journal Title: Biochimie Volume: 94 Pages: 2716-2722
• [Journal Article] Serine/Threonine kinase Melk regulates proliferation and glial differentiationof retinal progenitor cells (2012)
  • Author(s): Saito, R., Nakauchi, H., Watanabe, S.
  • Journal Title: Can. Sci. Volume: 103 Pages: 42-49
  • Peer Reviewed
• [Journal Article] Selection of RNA aptamers against mouse embryonic stem cells (2012)
  • Author(s): Iwagawa, T et al.
  • Journal Title: Biochimie Volume: 94 Pages: 250-257
  • Peer Reviewed
• [Journal Article] Serine/Threonine kinase Melk regulates proliferation and glial differentiation of retinal progenitor cells (2012)
  • Author(s): Saito, R.et al.
  • Journal Title: Can. Sci. Volume: 103 Pages: 42-49
  • Peer Reviewed
• [Journal Article] Transposon mutagenesis identifies genes that transform neural stem cells intoglioma-initiating cells (2012)
  • Author(s): Koso, H. et al.
  • Journal Title: Proc.Natl.Acad.Sci.,USA Volume: 109 Pages: 2998-3007
  • Peer Reviewed
• [Journal Article] Expression of Sox4 and Sox11 is regulated by multiple mechanisms during retinal development (2012)
  • Author(s): Usui, A. et al.
  • Journal Title: FEBS Letters Volume: 587 Pages: 358-363
  • Peer Reviewed
• [Journal Article] Serine/Threonine kinase Melk regulates proliferation and glial differentiation of retinal progenitor cells. (2012)
  • Author(s): Saito, R., Nakauchi, H., Watanabe, S.
  • Journal Title: Can. Sci. Volume: 103 Pages: 42-49
  • Peer Reviewed
• [Journal Article] Dicer plays essential roles for retinal development by regulation of survival and differentiation (2011)
  • Author(s): Iida, A., Shinoe, T., Baba, Y., Mano, H., W atanabe, S.
  • Journal Title: Invest. Ophthalmol. Vis. Sci. Volume: 52 Pages: 3008-3017
• [Journal Article] Sox9is upstream of microRNA-140 in cartilage (2011)
  • Author(s): Nakamura, Y., He, X., Kato, H., Wakitani, S., Kobayashi, T., Watanabe, S., Iida, A., Tahara, H., Warman, M. L., Watanapokasin, R., Postlethwait, J.H.
  • Journal Title: Appl. Biochem. Biotechnol Volume: 166 Pages: 64-71
  • Peer Reviewed
• [Journal Article] 細胞移植による組織の再生とその実現化 をささえる技術開発 (2011)
  • Author(s): Journal of the japan society of appliedelectromagnetics and mechanics
  • Journal Title: 日本AEM学会誌 Volume: V 20 Pages: 583-588
• [Journal Article] Dicer plays essential roles for retinal development by regulation of survival and differentiation. (2011)
  • Author(s): Iida, A., Shinoe, T., Baba, Y., Mano, H., Watanabe, S.
  • Journal Title: Invest. Ophthalmol. Vis. Sci. Volume: 52 Pages: 3008-3017
  • Peer Reviewed
• [Journal Article] β-Catenin signaling regulates the timing of cell differentiation in mouse retinal progenitor cells, (2011)
  • Author(s): Ouchi, Y., Baba, Y., Koso, H., Taketo, M. M., Iwamoto, T., Aburatani, H., Watanabe, S.
  • Journal Title: Mol. Cell. Neurosci. Volume: 46 Pages: 770-780
  • Peer Reviewed
• [Journal Article] Sall3 plays essential roles in horizontal cell maturation through regulation of neurofilament expression levels. (2011)
  • Author(s): Baba, Y., Iida, A., Watanabe, S.
  • Journal Title: Biochimie, Volume: 93 Pages: 1037-1046
  • Peer Reviewed
• [Journal Article] The role of Zic family zinc finger transcription factors in the proliferation and differentiation of retinal progenitor cells. (2011)
  • Author(s): Watabe, Y., Baba, Y., Nakauchi, H., Mizota, A., Watanabe, S.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 415 Pages: 42-47
  • Peer Reviewed
• [Presentation] Studies of retinal cell lineage and differentiation mechanisms by using cell surface antigens (2012)
  • Author(s): Watanabe, S
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2012-07-22
• [Presentation] Identification of the expression pattern of microRNAs in iPS cells
  • Author(s): Siti Razila, Watanabe, S
  • Organizer: and analysis of their roles in maintenance of iPS cell immaturityISRF 2012
  • Place of Presentation: Omaha, USA
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/moldev/