| Project/Area Number |
23659150
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAWASHITA Jinko 信州大学, 医学系研究科, 助教 (40359732)
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| Co-Investigator(Renkei-kenkyūsha) |
KAMETANI Fuyuki 公益財団法人東京都医学総合研究所, 認知症・高次脳機能研究分野, 主席研究員 (70186013)
KAKUTA Shigeru 東京大学, 農学生命科学研究科, 准教授 (80345032)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 加齢医学 / 老化 / アミロイド / 疾患モデル動物 / 質量分析 / 蛋白質 / プロテオーム解析 / 凝集体 / アミロイドーシス / タンパク質 / 恒常性 / プロテオーム / マウス / ApoA-II |
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| Research Abstract |
Comprehensive and quantitative proteome analysis using LS/MS/MS system was performed to amyloid fibrils /amyloid like aggregates protein fractions which were isolated form 1) the brain, liver, heart and muscle of young and old mice, and 2) the livers of mice induced amyloidosis with the injection of amyloid fibrils.
1) Over 40 proteins which were increased significantly in the old mouse brain were identified in amyloid fibrils/amyloid like aggregates fractions.
2) Apolipoprotein E, clusterin, vetronectin and polyubiquitin were increased in amyloid fractions associated with AApoAII amyloid deposition in the lever.
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