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Analysis of AID expression by a novel Cre-based in vivo gene manipulation system.

Research Project

Project/Area Number 23659151
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionGifu University

Principal Investigator

NAGAOKA Hitoshi  岐阜大学, 医学(系)研究科(研究院), 教授 (20270647)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsリンパ球分化 / 免疫 / 遺伝子 / 発現制御 / リンパ球 / 遺伝子組換え / 転写因子 / 細胞分化 / 生体分子医学 / ゲノム医科学
Research Abstract

We studied the Aicda (activation-induced cytidine deaminase's gene) gene regulation in vivo by using Aicda-cre BAC transgenic system. We found that the Aicda gene is expressed in some mature T cells in periphery in addition to the germinal center B cells. The AID expression associated with the development of a IL-10-producing subset of CD4 T cells, that indicates some specific stimuli may be operating for induction of AID. The manipulations of cis regulatory elements of Aicda on the transgenes revealed that indispensable role of C/EBP binding sites in the upstream region for AID expression, and the silencer activity of intronic E2F and Myb binding sites. These results provide a important information to understanding spatiotemporal regulation of Aicda gene.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] Mitotic catastrophe and cell deathinduced by depletion of centrosomalproteins2013

    • Author(s)
      Kimura, M., Yoshioka, T., Saio, M.,Banno, Y., Nagaoka, H., and Okano, Y
    • Journal Title

      Cell Death Dis

      Volume: 4 Issue: 4 Pages: e603-e603

    • DOI

      10.1038/cddis.2013.108

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo analysis of Aicda gene regulation: a critical balance between upstream enhancers and intronic silencers governs appropriate expression2013

    • Author(s)
      Le Thi Huong, Maki Kobayashi,et al
    • Journal Title

      PLoSONE

      Volume: vol.8 Issue: 4 Pages: e61433-e61433

    • DOI

      10.1371/journal.pone.0061433

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The AID Dilemma: Infection, or Cancer?2013

    • Author(s)
      Tasuku Honjo, Maki Kobayashi, Nasim Begum, Ai Kotani, Somayeh Sabour and Hitoshi Nagaoka
    • Journal Title

      Adv Cancer Res

      Volume: vol.113 Pages: 1-44

    • DOI

      10.1016/b978-0-12-394280-7.00001-4

    • ISBN
      9780123942807
    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a Rho family specificguanine nucleotide exchange factorFLJ00018, as a novel actin-bindingprotein2013

    • Author(s)
      Sato, K., Handa, H., Kimura, M., Okano,Y., Nagaoka, H., Nagase, T., Sugiyama,T., Kitade, Y., and Ueda, H.
    • Journal Title

      Cell. Signal.

      Volume: 25 Issue: 1 Pages: 41-49

    • DOI

      10.1016/j.cellsig.2012.09.015

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Imatinib mesylate directly impairs class switch recombination through downregulation of AID.2012

    • Author(s)
      Kawamata T, Lu J, Sato T, Tanaka M, Nagaoka H, Agata Y, Toyoshima T, Yokoyama K, Oyaizu N, Nakamura N, Ando K, Tojo A, Kotani A
    • Journal Title

      Blood

      Volume: 119 Issue: 13 Pages: 3123-3127

    • DOI

      10.1182/blood-2011-01-327932

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Activation-Induced Cytidine Deaminase Expression in CD4 T Cells is Associated with a Unique IL-10-Producing Subset that Increases with Age2011

    • Author(s)
      Qin, H., 他
    • Journal Title

      PLoS One

      Volume: 6(電子出版) Issue: 12 Pages: e29141-e29141

    • DOI

      10.1371/journal.pone.0029141

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Histone chaperone Spt6 is required for class switch recombination but not somatic hypermutation2011

    • Author(s)
      Okazaki IM, Okawa K, Kobayashi M, Yoshikawa K, Kawamoto S, Nagaoka H, Shinkura R, Kitawaki Y, Taniguchi H, Natsume T, Iemura S, and Honjo T
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108 Issue: 19 Pages: 920-925

    • DOI

      10.1073/pnas.1104423108

    • NAID

      120003163668

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Rho 活性化因子FLJ00018 とFour and Half LIM domain 1との相互作用2013

    • Author(s)
      佐藤克哉, 他
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜市
    • Year and Date
      2013-09-12
    • Related Report
      2013 Final Research Report
  • [Presentation] In vivo analysis of Aicda gene regulation : critical balance between upstream enhancer and intronic repressor for the appropriate expression2013

    • Author(s)
      Nagaoka, H., et al.
    • Organizer
      15th International Congress of Immunology
    • Place of Presentation
      Milan, Italy
    • Year and Date
      2013-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] In vivo analysis of Aicda gene regulation: critical balance between upstream enhancer and intronic repressor for the appropriate expression.2013

    • Author(s)
      L. Huong, M. Kobayashi, M. Nakata, G. Shioi, H. Miyachi, T. Honjo, H. Nagaoka
    • Organizer
      15th international congress of immunology
    • Place of Presentation
      Milan, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] n vivo analysis of Aicda gene regulation: critical balance between upstream enhancer and intronic repressor for the appropriate expression2013

    • Author(s)
      L. Huong, M. Kobayashi, M. Nakata, G. Shioi, H. Miyachi, T. Honjo, H. Nagaoka
    • Organizer
      15th International Congress of Immunology
    • Place of Presentation
      Milan, Italy
    • Related Report
      2012 Research-status Report
  • [Presentation] Rho 特異的活性化因子FLJ00018 とアクチンの相互作用によるFLJ00018 の機能抑制2012

    • Author(s)
      佐藤克哉, 他
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡市
    • Year and Date
      2012-12-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Activation-induced cytidine deaminase expression in CD4+ T cells is associated with a unique IL- 10-producing subset that increases with age2012

    • Author(s)
      Nagaoka, H, et al.
    • Organizer
      Genetic and Epigenetic Control of Cell Fate
    • Place of Presentation
      京都市
    • Year and Date
      2012-11-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Activation-induced cytidine deaminase expression in CD4+ T cells is associated with a unique IL-10-producing subset that increases with age2012

    • Author(s)
      Hongyan Qin, Keiichiro Suzuki, Mikiyo Nakata, Shunsuke Chikuma, Nakako Izumi, Le Thi Huong, Mikako Maruya, Sidonia Fagarasan, Meinrad Busslinger, Tasuku Honjo and Hitoshi Nagaoka
    • Organizer
      Genetic and Epigenetic Control of Cell Fate
    • Place of Presentation
      京都市
    • Related Report
      2012 Research-status Report
  • [Presentation] AID 遺伝子が発現するT細胞サブセットはIL-10 を発現し加齢とともに蓄積するユニークなサブセットである2011

    • Author(s)
      長岡 仁, 他
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市
    • Year and Date
      2011-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] RNF8 によるPlk1 タンパク質の減少とがん細胞における異常2011

    • Author(s)
      吉岡 孝, 他
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市
    • Year and Date
      2011-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] AID遺伝子が発現するT細胞サブセットはIL-10を発現し加齢とともに蓄積するユニークなサブセットである

    • Author(s)
      長岡 仁. 他
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市
    • Related Report
      2011 Research-status Report
  • [Presentation] RNF8によるPlk1タンパク質の減少とがん細胞における異常

    • Author(s)
      吉岡 孝 他
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.med.gifu-u.ac.jp/labo/labo-j/molpathobiochem.html

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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