Comprehensive Analysis of Ubiquitome during DNA damage response

• Project/Area Number: 23659152
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: TAKEDA Shunichi 京都大学, 大学院・医学研究科, 教授 (60188191)
• Co-Investigator(Kenkyū-buntansha): SASANUMA Hiroyuki 京都大学, 大学院・医学研究科, 准教授 (00531691)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: プロテオミクス / ユビキチン / ユビキトーム / DNA修復 / 質量分析 / SILAC / プロテオノミクス

Research Abstract

The objective of this study is to establish the protocol thatensures the sensitive analysis of ubiquitome for the chicken DT40 cell line. We havemet the objective, and identified a number of ubiquitylated proteins.

Research Products

• [Journal Article] Interference in DNA replieation can cause mitotic chromosomal breakage unassociated with double-strand breaks (2013)
  • Author(s): Fujita Mari, Sasanuma H, Kimiyo N. Yamamoto Harada H, Kurosawa A, Adachi N, Omura M, Hiraoka M, Takeda S, Hirota K.
  • Journal Title: PLos One Volume: 8 Issue: 4 Pages: 60043-60043
  • DOI: 10.1371/journal.pone.0060043
  • Peer Reviewed
• [Presentation] 遺伝学的手法を応用した、新規抗がん剤のスクリーニングとその作用機序の解析 (2013)
  • Author(s): 武田俊一
  • Organizer: 金沢大学薬学シンポジウム2012-DNA損傷応答を標的とした革新的がん創薬研究-
  • Place of Presentation: 金沢
  • Year and Date: 2013-02-19
• [Presentation] 遺伝学的手法を応用した、新規抗がん剤のスクリーニングとその作用機序の解析 (2013)
  • Author(s): 武田 俊一
  • Organizer: 金沢大学薬学シンポジウム2012 -DNA損傷応答を標的とした革新的がん創薬研究-
  • Place of Presentation: 金沢
  • Invited
• [Presentation] Xpf and Mus81 are requiredto process homologous recombinationintermediates (2012)
  • Author(s): Takeda S
  • Organizer: EMBO Workshop,Structure-specific nucleases in DNAreplication and repair
  • Place of Presentation: Hyeres, France.
  • Year and Date: 2012-09-19
• [Presentation] Genetic and Biocheicalevidence for translesion DNAsynthesis by replicative DNApolymerase delta (2012)
  • Author(s): Takeda S
  • Organizer: Gordon ResearchConference, Mutagenesis
  • Place of Presentation: Newport, USA
• [Presentation] Genetic and Biocheical evidence for translesion DNA synthesis by replicative DNA polymerase delta (2012)
  • Author(s): Takeda S
  • Organizer: Gordon Research Conference, Mutagenesis
  • Place of Presentation: Newport, USA
• [Presentation] Xpf and Mus81 are required to process homologous recombination intermediates (2012)
  • Author(s): Takeda S
  • Organizer: EMBO Workshop, Structure-specific nucleases in DNA replication and repair
  • Place of Presentation: Hyeres, France
• [Presentation] Complex functionalrelationship of the UBC13 E2ubiquitin conjugating enzyme, and theRAD18 and RNF8 E3 ubiquitin ligases,FASEB Summer Research Conferences (2011)
  • Author(s): Takeda S
  • Organizer: Genetic Recombination & GenomeRearrangements
  • Place of Presentation: Steamboat Springs, USA
• [Presentation] Complex functional relationship of the UBC13 E2 ubiquitin conjugating enzyme, and the RAD18 and RNF8 E3 ubiquitin ligases. (2011)
  • Author(s): Takeda S, Kobayashi S
  • Organizer: FASEB Summer Research Conferences, Genetic Recombination & Genome Rearrangements
  • Place of Presentation: Steamboat Springs（アメリカ）