Translational control of glucose-induced insulin biosynthesis
Project/Area Number |
23659161
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Nara Medical University |
Principal Investigator |
TAKASAWA Shin 奈良県立医科大学, 医学部, 教授 (50187944)
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Co-Investigator(Kenkyū-buntansha) |
HIRONAKA Asako 奈良県立医科大学, 医学部, 講師 (80211514)
TSUCHIDA Sumiyo 奈良県立医科大学, 医学部, 教務職員 (70458016)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | インスリン / 翻訳調節 / 糖尿病 / インスリン遺伝子 / ブドウ糖 / 膵β細胞 |
Research Abstract |
Glucose-induced insulin synthesis is important for homeostasis of blood glucose level and regulated by transcription, translation, and secretion. In the present study, we fused several lengths of 5’-untranslated region of human insulin mRNA with protein-destabilization sequence (PEST sequence). We then introduced the fused genes into glucose-sensitive human insulin producing cells (1.1B4 cells) and determined mRNA sequence essential for glucose-induced pro-insulin synthesis by translation. The sequence “GUCCUUCUG” in the 5’-untranslated region of human insulin mRNA was indicated as a sequence essential for translational control of glucose-induced pro-insulin synthesis. The human (Homo sapiens sapiens) sequence was highly conserved in chimpanzee (Pan troglodytes), rat (Rattus norvegicus), mouse (Mus musculus), guinea pig (Cavia Porcellus), pig (Sus scrofa domesticus), bovine(Bos taurus), chicken (Gallus gallus domesticus), leopard frog (Rena pipiens), and zebrafish (Danio rerio) insulin mRNAs; The sequence “NYNSYYNR “ was suggested as consensus sequence for glucose-induced translational control of insulin biosynthesis.
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Report
(3 results)
Research Products
(109 results)
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[Journal Article] Prevention of Reg protein-induced β-cell apoptosis by induction of HGF gene transcription2013
Author(s)
Nakagawa, K., Takasawa, S., Nata, K., Yamauchi, A., Itaya-Hironaka, A., Ota, H., Yoshimoto, K., Sakuramoto-Tsuchida, S., Miyaoka, T., Unno, M. and Okamoto, H
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[Journal Article] Expression of REG III and prognosis in head and neck cancer2013
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Masui, T., Ota, I., Itaya-Hironaka, A., Takeda, M., Kasai, T., Yamauchi, A., Sakurakoto-Tsuchida, S., Mikami, S., Yane, K., Takasawa, S. and Hosoi, H
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[Journal Article] Involvement of autoimmunity to REG, a regeneration factor, in patients with primary Sjogren's syndrome2013
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Yoshimoto, K., Fujimoto, T., Itaya-Hironaka, A., Miyaoka, T., Sakuramoto-Tsuchida, S., Yamauchi, A., Fujimura, T., Takeda, M., Kasai, T., Nakagawara, K., Nonomura, A. and Takasawa, S.
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[Journal Article] Reg 3γ gene expression in regenerating skeletal muscle and corresponding nerve2013
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Klasan, G.S., Ivanac, D., Erzen, D.J., Picard, A., Takasawa, S., Peharec, S., Arbanas, J., Girotto, D. and Jerkovic, R
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[Journal Article] E. Biomarkers for diagnosis and monitoring of Celiac disease2013
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Vives-Pi, M., Takasawa, S., Pujol-Autonell, I., Planas, R., Cabre, E., Ojanguren, I., Montraveta, M. Santos, A. L. and Ruiz-Oritz
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Journal Title
J. Clin. Gastroenterol
Volume: vol. 47
Pages: 308-313
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[Journal Article] Identification of a major enzyme for the synthesis and hydrolysis of cyclic ADP-ribose in amphibian cells and evolutional conservation of the enzyme from human to invertebrate2012
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Ikeda, T., Takasawa, S., Noguchi, N., Nata, K., Yamauchi, A., Takahashi, I., Yoshikawa, T., Sugawara, A., Yonekura, H. and Okamoto,H.
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Journal Title
Mol. Cell. Biochem
Volume: vol. 366
Pages: 69-80
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[Journal Article] Attenuation of glucose-induced insulin secretion by intermittent hypoxia via down-regulation of CD382012
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Ota, H., Tamaki, S., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Morioka, T., Takasawa, S. and Kimura, H
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Journal Title
Life Sci
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[Journal Article] Vascular smooth muscle cell proliferation by intermittent hypoxia is independent on the glucose-induced insulin secretion.2012
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Kyotani, Y., Ota, H., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Zhao, J., Kimura, H., Uno, M., Takasawa, S. and Yoshizumi, M.
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[Journal Article] Identification of a major enzyme for the synthesis and hydrolysis of cyclic ADP-ribose in amphibian cells and evolutional conservation of the enzyme from human to invertebrate.2012
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Ikeda, T., Takasawa, S., Noguchi, N., Nata, K., Yamauchi, A., Takahashi, I., Yoshikawa, T., Sugawara, A., Yonekura, H. and Okamoto, H.
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Mol. Cell. Biochem.
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[Journal Article] Septic shock is associated with receptor for advanced glycation endproducts (RAGE) ligation of LPS2011
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Yamamoto, Y., Harashima, A., Saito, H., Tsuneyama, K., Munesue, S., Motoyoshi, S., Han, D., Watanabe, T., Asano, M., Takasawa, S., Okamoto, H., Shimura, S., Karasawa, T., Yonekura, H. and Yamamoto, H
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Journal Title
J. Immunol
Volume: vol. 186
Pages: 3248-3257
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[Journal Article] Expression profiling of the REG gene family in colorectal carcinoma2011
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Zheng, H.C., Sugawara, A., Okamoto, H., Takasawa, S., Takahashi, H., Masuda, S. and Takano, Y
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J. Histochem. Cytochem
Volume: vol. 59
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[Journal Article] Deletion of platelet-derived growth factor-β receptor improves diabetic nephropathy in Ca2+/calmodulin-dependent protein kinase IIα(Thr286Asp) transgenic mice2011
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Suzuki, H., Usui, I., Kato, I., Oya, T., Kanatani, Y., Yamazaki, Y., Fujisaka, S., Senda, S., Ishii, Y., Urakaze, M., Mahmood, A., Takasawa, S., Okamoto, H., Kobayashi, M., Tobe, K. and Sasahara, M
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Diabetologia
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[Journal Article] The effect of mesenchymal stem cell osteoblastic differentiation on the mechanical properties of engineered bone-like tissue2011
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Naito, H., Dohi, Y. Zimmermann, W.-H., Tojo, T., Takasawa, S., Eschenhagen, T. and Taniguchi, S
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Journal Title
Tissue Engineering
Volume: vol. 17
Pages: 2321-2329
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[Journal Article] Septic shock is associated with receptor for advanced glycation endproducts (RAGE) ligation of LPS.2011
Author(s)
Yamamoto, Y., Harashima, A., Saito, H., Tsuneyama, K., Munesue, S., Motoyoshi, S., Han, D., Watanabe, T., Asano, M., Takasawa, S. et al.
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Journal Title
J. Immunol.
Volume: 186
Pages: 3248-3257
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[Journal Article] Expression profiling of the REG gene family in colorectal carcinoma.2011
Author(s)
Zheng, H.C., Sugawara, A., Okamoto, H., Takasawa, S., Takahashi, H., Masuda, S. and Takano, Y.
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Journal Title
J. Histochem. Cytochem.
Volume: 59
Pages: 106-115
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[Journal Article] Deletion of platelet-derived growth factor-β; receptor improves diabetic nephropathy in CaM kinase IIα (Thr286Asp) transgenic mice.2011
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Suzuki, H., Usui, I., Kato, I., Oya, T., Kanatani, Y., Yamazaki, Y., Fujisaka, S., Senda, S., Ishii, Y., Urakaze, M., Takasawa, S., Okamoto, H., Kobayashi, M., Tobe, K. and Sasahara, M.
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Journal Title
Diabetologia
Volume: 54
Pages: 2953-2962
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[Journal Article] The effect of mesenchymal stem cell osteoblastic differentiation on the mechanical properties of engineered bone-like tissue.2011
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Naito, H., Dohi, Y. Zimmermann, W.-H., Tojo, T., Takasawa, S., Eschenhagen, T. and Taniguchi, S.
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Tissue Engineering
Volume: 17
Pages: 2321-2329
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[Journal Article] IL-6/Dexamethasone activated the human REG Iα gene transcription in pancreatic β-cells via the JAK/STAT signaling pathway.2011
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Yamauchi, A., Itaya-Hironak, A., Sakuramoto-Tsuchida, S., Yoshimoto, K., Miyaoka, T., Ota, H., Naito, H. and Takasawa, S.
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[Journal Article] Attenuation of glucose-induced insulin secretion by intermittent hypoxia via down-regulation of CD38.2011
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Ota, H., Tamaki, S., Itaya-Hironak, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Takasawa, S. and Kimura, H.
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[Journal Article] Involvement of autoimmunity to REG in patients with primary Sjοgren’s syndrome.2011
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Yoshimoto, K., Fujimoto, T., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Miyaoka, T., Takeda, M., Kasai, T., Nonomura, A. and Takasawa, S.
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Volume: 70
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[Journal Article] Attenuation of glucose-induced insulin secretion from pancreatic beta cells by intermittent hypoxia via down-regulation of CD38.2011
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Ota, H., Tamaki, S., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Morioka, T., Takasawa, S. and Kimura, H.
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[Journal Article] 睡眠時無呼吸/ガス交換 周期的間歇的低酸素による膵β細胞の機能不全と増殖:睡眠時無呼吸症候群と糖尿病との関連の可能性2011
Author(s)
Ota, H., Tamaki, S., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Morioka, T., Takasawa, S. and Kimura, H.
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日本呼吸器病学会雑誌
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[Presentation] Induction of REG Iα, a new auto-antigen in Sjogren's syndrome patients, in salivary duct epithelial cells by interleukin-6 and -112013
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[Presentation] Intermittent hypoxia stimulates βcell proliferation via up-regulation of Regfamily genes2012
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[Presentation] The JAK/STAT signaling is essential for human REG Iα transcription in pancreatic β cells2012
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[Presentation] Pancreatic β cell proliferation by intermittent hypoxia via up-regulation of Reg family genes2012
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[Presentation] Intermittent hypoxia promotes rat vascular muscle cells proliferation with up-regulation of erbB2 receptor and epidermal growth factor family2012
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[Presentation] CD38 SNP (R140W) is associated with an increased risk of admission to the neonatal intensive care unit2012
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[Presentation] Vascular smooth muscle cell proliferation by intermittent hypoxia is independent on the glucose-induced insulin secretion2012
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The 72nd American Diabetes Association Scientific Sessions
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[Presentation] CD38 SNP (R140W) is associated with an increased risk of admission to the neonatal intensive care unit.2012
Author(s)
Takasawa, S., Enami, N., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Miyaoka, T., Yoshimoto, K., Tsujinaka, H., Fujimura, T. and Takahashi, Y.
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Asian CD38 and NAD Meeting(招待講演)
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Jeju, Korea
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[Presentation] Involvement of autoimmunity to REG in patients with primary Sjogren's syndrome2011
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Fujimoto, T., Yoshimoto, K., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Miyaoka, T., Takeda, M., Kasai, T., Nonomura, A., Tanaka, Y. and Takasawa, S
Organizer
11thInternational Symposium on Sjogren's syndrome
Place of Presentation
Athens, Greece
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[Presentation] Attenuation of glucose-induced insulin secretion from pancreatic beta cells by intermittent hypoxia via down-regulation of CD382011
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Ota, H., Tamaki, S., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Morioka, T., Takasawa, S. and Kimura, H
Organizer
47thEASD Meeting
Place of Presentation
Lisbon, Portugal
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[Presentation] Involvement of autoimmunity to REG in patients with primary Sjogren's syndrome2011
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Yoshimoto, K., Fujimoto, T., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Miyaoka, T., Takeda, M., Kasai, T., Nonomura, A. and Takasawa, S
Organizer
Annual European Congress of Rheumatology EULAR 2011
Place of Presentation
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[Presentation] REG family genes expression in lung cancer2011
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Naito, H., Tojo, T., Kimura, M., Kawaguchi, T., Takasawa, S., Itaya-Hironaka, A., Dohi, Y. and Taniguchi, S
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International Association for the Study of Lung Cancer 2011
Place of Presentation
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[Presentation] Attenuation of glucose-induced insulin secretion by intermittent hypoxia via down-regulation of CD382011
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Ota, H., Tamaki, S., Itaya-Hironak, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Takasawa, S. and Kimura, H
Organizer
The 71stAmerican Diabetes Association Scientific Sessions
Place of Presentation
San Diego, CA, USA
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[Presentation] IL-6/Dexamethasone activated the human REG Iα gene transcription in pancreatic β-cells via the JAK/STAT signaling pathway2011
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Yamauchi, A., Itaya-Hironak, A., Sakuramoto-Tsuchida, S., Yoshimoto, K., Miyaoka, T., Ota, H., Naito, H. and Takasawa, S
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The 71stAmerican Diabetes Association Scientific Sessions
Place of Presentation
San Diego, CA, USA
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[Presentation] Dysfunction and proliferation of pancreatic beta-cells by intermittent hypoxia2011
Author(s)
Takasawa, S., Ota, H., Tamaki, S., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Yoshimoto, K., Miyaoka, T. and Kimura, H
Organizer
Meting for the world-class research hub in East Asia on cADPR and CD38 (Winter conference in Kanazawa 2011)
Place of Presentation
Kaga-shi, Japan (Invited Lecture)
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[Presentation] 睡眠時無呼吸/ガス交換 周期的間歇的低酸素による膵 β 細胞の機能不全と増殖:睡眠時無呼吸症候群と糖尿病との関連の可能性2011
Author(s)
Ota, H., Tamaki, S., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Morioka, T., Takasawa, S. and Kimura, H
Organizer
第51回日本呼吸器学会学術講演会
Place of Presentation
東京
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[Presentation] 睡眠時無呼吸/ガス交換 周期的間歇的低酸素による膵β細胞の機能不全と増殖:睡眠時無呼吸症候群と糖尿病との関連の可能性2011
Author(s)
Ota, H., Tamaki, S., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Morioka, T., Takasawa, S. and Kimura, H.
Organizer
第51回日本呼吸器学会学術講演会
Place of Presentation
東京
Related Report
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[Presentation] IL-6/Dexamethasone activated the human REG Iα gene transcription in pancreatic β-cells via the JAK/STAT signaling pathway.2011
Author(s)
Yamauchi, A., Itaya-Hironak, A., Sakuramoto-Tsuchida, S., Yoshimoto, K., Miyaoka, T., Ota, H., Naito, H. and Takasawa, S.
Organizer
The 71st American Diabetes Association Scientific Sessions
Place of Presentation
San Diego, CA
Related Report
-
[Presentation] Attenuation of glucose-induced insulin secretion by intermittent hypoxia via down-regulation of CD38.2011
Author(s)
Ota, H., Tamaki, S., Itaya-Hironak, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Takasawa, S. and Kimura, H.
Organizer
The 71st American Diabetes Association Scientific Sessions
Place of Presentation
San Diego, CA
Related Report
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[Presentation] Involvement of autoimmunity to REG in patients with primary Sjοgren’s syndrome.2011
Author(s)
Yoshimoto, K., Fujimoto, T., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Miyaoka, T., Takeda, M., Kasai, T., Nonomura, A. and Takasawa, S.
Organizer
Annual European Congress of Rheumatology EULAR 2011
Place of Presentation
London, UK
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[Presentation] Attenuation of glucose-induced insulin secretion from pancreatic beta cells by intermittent hypoxia via down-regulation of CD38.2011
Author(s)
Ota, H., Tamaki, S., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Morioka, T., Takasawa, S. and Kimura, H.
Organizer
47th EASD Meeting
Place of Presentation
Lisbon, Portugal
Related Report
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[Presentation] Involvement of autoimmunity to REG in patients with primary Sjοgren’s syndrome.2011
Author(s)
Fujimoto, T., Yoshimoto, K., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Miyaoka, T., Takeda, M., Kasai, T., Nonomura, A., Tanaka, Y. and Takasawa, S.
Organizer
11th International Symposium on Sjögren’s syndrome(招待講演)
Place of Presentation
Athens, Greece
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