Drawing a differentiation potency index of iPSC based on genomic imprinting

• Project/Area Number: 23659181
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human genetics
• Research Institution: Saga University
• Principal Investigator: SOEJIMA Hidenobu 佐賀大学, 医学部, 教授 (30304885)
• Co-Investigator(Renkei-kenkyūsha): OGURA Atsuo 独立行政法人理化学研究所, 遺伝工学基盤技術室, 室長 (20194524)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: エピジェネティクス / ゲノム刷り込み / iPS細胞 / 肝芽腫 / DNAメチル化 / インプリントDMR / ゲノムインプリンティング / 分化万能性

Research Abstract

To evaluate iPSC from the aspect of genomic imprinting, we analyzed DNA methylation status of imprinting related differentially methylated regions (DMRs), which were scattered throughout the genome, in iPSCs derived from mouse fibroblast, peripheral blood cells of normal human and Beckwith-Wiedemann syndrome patients. We also analyzed hepatoblastomas, which originated from immature liver precursor cells. We found that in mouse iPSC, the methylation status of many DMRs decreased after reprogramming and that a certain number of DMRs restored their methylation status during retinoic acid induced differentiation. Human iPSC showed that methylation patterns of several DMRs changed to paternal epigenotype after reprogramming. Finally, hepatoblastoma analysis suggested that the occurrence of hypomethylation at a few DMRs prior to tumor development.

Research Products

• [Journal Article] Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation (2014)
  • Author(s): Ohnishi K, Semi K, Yamamoto T, Shimizu M, Tanaka A, Mitsunaga K, Okita K, Osafune K, Arioka Y, Maeda T, Soejima H, Moriwaki H, Yamanaka S, Woltjen K, Yamada Y
  • Journal Title: Cell Volume: 156(4) Pages: 663-677
  • Peer Reviewed
• [Journal Article] Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation. (2014)
  • Author(s): Ohnishi K, Semi K, Yamamoto T, Shimizu M, Tanaka A, Mitsunaga K, Okita K, Osafune K, Arioka Y, Maeda T, Soejima H, Moriwaki H, Yamanaka S, Woltjen K, Yamada Y.
  • Journal Title: Cell Volume: 156 Issue: 4 Pages: 663-677
  • DOI: 10.1016/j.cell.2014.01.005
  • Peer Reviewed
• [Journal Article] A novel de novo point mutation of the OCT-binding site in the IGF2/H19-imprinting control region in a Beckwith-Wiedemann syndrome patient (2013)
  • Author(s): Higashimoto K, Jozaki K, Kosho T, Matsubara K, Fuke T, Yamada D, Yatsuki H, Maeda T, Ohtsuka Y, Nishioka K, Joh K, Koseki H, Ogata T, Soejima H
  • Journal Title: Clin Genet Volume: (published online)
  • Peer Reviewed
• [Journal Article] Comprehensive analyses of imprinted differentially methylated regions reveal epigenetic and genetic characteristics in hepatoblastoma (2013)
  • Author(s): Rumbajan JM, Maeda T, Souzaki R, Mitsui K, Higashimoto K, Nakabayashi K, Yatsuki H, Nishioka K, Harada R, Aoki S, Kohashi K, Oda Y, Hata K, Saji T, Taguchi T, Tajiri T, Soejima H, Joh K
  • Journal Title: BMC Cancer Volume: 13 Pages: 608-608
  • Peer Reviewed
• [Journal Article] Ash1l methylates Lys36 of histone H3 independently of transcriptional elongation to counteract Polycomb silencing (2013)
  • Author(s): Miyazaki H, Higashimoto K, Yada Y, A. Endo T¶, Sharif J¶, Komori T, Matsuda M, Koseki Y, Nakayama M, Soejima H, Handa H, Koseki H, Hirose S, Nishioka K
  • Journal Title: PLoS Genet Volume: 9(11)
  • Peer Reviewed
• [Journal Article] Homozygous deletion of DIS3L2 exon 9 due to non-allelic homologous recombination between LINE-1s in a Japanese patient with Perlman syndrome (2013)
  • Author(s): Higashimoto K, Maeda T, Okada J, Ohtsuka Y, Sasaki K, Hirose A, Nomiyama M, Takayanagi T, Fukuzawa R, Yatsuki H, Koide K, Nishioka K, Joh K, Watanabe Y, Yoshiura KI, Soejima H
  • Journal Title: Eur J Hum Genet Volume: 21(11) Pages: 1316-1319
  • Peer Reviewed
• [Journal Article] Epigenetic and genetic alterations of the imprinting disorder Beckwith-Wiedemann syndrome and related disorders (2013)
  • Author(s): Soejima H, Higashimoto K
  • Journal Title: J Hum Genet Volume: 58(7) Pages: 402-409
  • NAID: 10031190033
  • Peer Reviewed
• [Journal Article] Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes (2013)
  • Author(s): Fukuda K, Ichiyanagi K, Yamada Y, Go Y, Udono T, Wada S, Maeda T, Soejima H, Saitou N, Ito T, Sasaki H
  • Journal Title: J Hum Genet Volume: 58(7) Pages: 446-454
  • NAID: 10031190040
  • Peer Reviewed
• [Journal Article] Congenital hyperinsulinism in an infant with paternal uniparental disomy on chromosome 11p15 : Few clinical features suggestive of Beckwith-Wiedemann syndrome (2013)
  • Author(s): Adachi H, Takahashi I, Higashimoto K, Tsuchida S, Noguchi A, Tamura H, Arai H, Ito T, Masue M, Nishibori H, Takahashi T, Soejima H
  • Journal Title: Endocr J Volume: 60(4) Pages: 403-408
  • NAID: 10031170628
  • Peer Reviewed
• [Journal Article] Novel Mutations of CDKN1C in Japanese Patients with Beckwith-Wiedemann Syndrome (2013)
  • Author(s): Yatsuki H, Higashimoto K, Jozaki K, Koide K, Okada J, Watanabe Y, Okamoto N, Tsuno Y, Yoshida Y, Ueda K, Shimizu K, Ohashi H, Mukai T, Soejima H
  • Journal Title: Genes & Genomics Volume: 35(2) Pages: 141-147
  • Peer Reviewed
• [Journal Article] Beckwith-Wiedemann 症候群とSilver-Russell 症候群 (2013)
  • Author(s): 前田寿幸, 東元健, 副島英伸
  • Journal Title: 小児科臨床 Volume: 66(増刊号) Pages: 1308-1314
• [Journal Article] Beckwith-Wiedemann 症候群, Sotos 症候群 (2013)
  • Author(s): 副島英伸
  • Journal Title: 周産期医学 Volume: 43(3) Pages: 377-382
• [Journal Article] Epigenetic and genetic alterations of the imprinting disorder Beckwith-Wiedemann syndrome and related disorders. (2013)
  • Author(s): Soejima H, Higashimoto K.
  • Journal Title: J Hum Genet Volume: 58 Issue: 7 Pages: 402-409
  • DOI: 10.1038/jhg.2013.51
  • NAID: 10031190033
  • Peer Reviewed
• [Journal Article] Regional DNA Methylation Differences between Humans and Chirnpanzees Are Associated with Genetic Changes, Transeriptional Divergence and Disease Genes (2013)
  • Author(s): 福田 渓
  • Journal Title: J. Hum, Genet Volume: 58 Issue: 7 Pages: 446-54
  • DOI: 10.1038/jhg.2013.55
  • Peer Reviewed
• [Journal Article] Ash1l methylates Lys36 of histone H3 independently of transcriptional elongation to counteract polycomb silencing (2013)
  • Author(s): H. Miyazaki, K. Higashimoto, Y. Yada, T. A. Endo, J. Sharif, T. Komori, M. Matsuda, Y. Koseki, M. Nakayama, H. Soejima, H. Handa, H. Koseki, S. Hirose, K. Nishioka
  • Journal Title: PLoS Genet. Volume: 9 Issue: 11 Pages: e1003897-e1003897
  • DOI: 10.1371/journal.pgen.1003897
  • Peer Reviewed
• [Journal Article] Comprehensive analyses of imprinted differentially methylated regions reveal epigenetic and genetic characteristics in hepatoblastoma (2013)
  • Author(s): Rumbajan JM, Maeda T, Souzaki R, Mitsui K, Higashimoto K, Nakabayashi K, Yatsuki H, Nishioka K, Harada R, Aoki S, Kohashi K, Oda Y, Hata K, Saji T, Taguchi T, Tajiri T, Soejima H, Joh K.
  • Journal Title: BMC Cancer Volume: 13 Issue: 1 Pages: 608-619
  • DOI: 10.1186/1471-2407-13-608
  • Peer Reviewed / Open Access
• [Journal Article] A novel de novo point mutation of OCT-binding site in the IGF2/H19-imprinting control region in a patient with Beckwith-Wiedemann syndrome. (2013)
  • Author(s): Higashimoto K, Jozaki K, Kosho T, Matsubara K, Sato T, Yamada D, Yatsuki H, Maeda T, Ohtsuka Y, Nishioka K, Joh K, Koseki H, Ogata T, SoejimaH*
  • Journal Title: Clin Genet Volume: in press Issue: 6 Pages: 539-544
  • DOI: 10.1111/cge.12318
  • Peer Reviewed / Open Access
• [Journal Article] Beckwith-Wiedemann症候群とSilver-Russell症候群 (2013)
  • Author(s): 前田寿幸、東元健、副島英伸
  • Journal Title: 小児科臨床 Volume: 66 Pages: 1308-1314
• [Journal Article] Congenital hyperinsulinism in an infant with paternal uniparental disomy on chromosome 11p15: Few clinical features suggestive of Beckwith-Wiedemann syndrome (2013)
  • Author(s): Adachi H
  • Journal Title: Endocrine Journal Volume: 60 Issue: 4 Pages: 403-408
  • DOI: 10.1507/endocrj.EJ12-0242
  • NAID: 10031170628
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Journal Article] Homozygous deletion of DIS3L2 exon 9 due to non-allelic homologous recombination between LINE-is in a Japanese patient with Perlman syndrome. (2013)
  • Author(s): Higashimoto, K, Maeda, T, Okada, J, Ohtsuka, Y, Sasaki, K, Hirose, A, Nomiyama, M, Takayanagi, T, Fukuzawa, R, Yatsuki, H, Koide, K, Nishioka, K, Joh, K, Watanabe, Y, Yoshiura, ICI, Soejima, H.
  • Journal Title: European Journal of Human Genetics Volume: 21 Issue: 11 Pages: 1316-1319
  • DOI: 10.1038/ejhg.2013.45
  • Peer Reviewed
• [Journal Article] Multiple mucocutaneous (palisaded encapsulated) neuromas may be a distinct entity (2013)
  • Author(s): Misago N
  • Journal Title: Arch Dermatol Volume: in press
  • Peer Reviewed
• [Journal Article] Epigenetic and genetic alterations of the imprinting disorder Beckwith-Wiedemann syndrome (2013)
  • Author(s): Soejima H
  • Journal Title: J Hum Genet Volume: in press
  • Peer Reviewed
• [Journal Article] Beckwith-Wiedemann症候群，Sotos症候群 (2013)
  • Author(s): 副島英伸
  • Journal Title: 周産期医学 Volume: 43(3) Pages: 377-382
• [Journal Article] Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted reproduction technologies (2012)
  • Author(s): Hiura H, Okae H, Miyauchi N, Sato F, Sato A, Van De Pette M, John RM, Kagami M, Nakai K, Soejima H, Ogata T, Arima T
  • Journal Title: Hum Reprod Volume: 27(8) Pages: 2541-2548
  • Peer Reviewed
• [Journal Article] Aberrant methylation of H19-DMR acquired after implantation was dissimilar in soma versus placenta of patients with Beckwith-Wiedemann syndrome (2012)
  • Author(s): Higashimoto K, Nakabayashi K, Yatsuki H, Yoshinaga H, Jozaki K, Okada J, Watanabe Y, Aoki A, Shiozaki A, Saito S, Koide K, Mukai T, Hata K, Soejima H
  • Journal Title: Am J Med Genet Part A Volume: 158A(7) Pages: 1670-1675
  • Peer Reviewed
• [Journal Article] Aberrant methylation of H19-DMR acquired after implantation was dissimilar in soma versus placenta of patients with Beckwith-Wiedemann syndrome (2012)
  • Author(s): Higashimoto K
  • Journal Title: Am J Med Genet A Volume: 158A Issue: 7 Pages: 1670-1675
  • DOI: 10.1002/ajmg.a.35335
  • Peer Reviewed
• [Journal Article] Aberrant methylation of H19-DMR acquired after (2012)
  • Author(s): Higashimoto K, et al.
  • Journal Title: Am J Med Genet A Volume: in press
  • Peer Reviewed
• [Journal Article] Beckwith-Wiedemann Syndrome with Placental (2011)
  • Author(s): Aoki A, et al.
  • Journal Title: J Obstet Gynaecol Res Volume: 37 Issue: 12 Pages: 1872-1876
  • DOI: 10.1111/j.1447-0756.2011.01654.x
  • Peer Reviewed
• [Journal Article] Methylation dynamics of IG-DMR and Gtl2-DMR (2011)
  • Author(s): Sato S, et al.
  • Journal Title: Genomics Volume: 98 Issue: 2 Pages: 120-127
  • DOI: 10.1016/j.ygeno.2011.05.003
  • Peer Reviewed
• [Journal Article] Methylation screening of reciprocal genome-wide UPDs identifies novel human-specific imprinted genes (2011)
  • Author(s): Nakabayashi K, Aburatani H, et al.
  • Journal Title: Hum Mol Genet Volume: 20 Issue: 16 Pages: 3188-97
  • DOI: 10.1093/hmg/ddr224
  • Peer Reviewed
• [Journal Article] Tissue-specific demethylation in CpG-poor promoters during cellular differentiation (2011)
  • Author(s): Nagae G, Isagawa T, Shiraki N, Fujita T, Yamamoto S, Tsutsumi S, Nonaka A,Yoshiba S, Matsusaka K, Midorikawa Y,Ishikawa S, Soejima H, Fukayama M,Suemori H, Nakatsuji N, Kume S,Aburatani H
  • Journal Title: Hum Mol Genetics Volume: 20(14) Issue: 14 Pages: 2710-21
  • DOI: 10.1093/hmg/ddr170
  • Peer Reviewed
• [Presentation] Ash1l methylates Lys36 of histone H3 independently of transcriptional elongation to counteract Polycomb silencing (2013)
  • Author(s): 宮崎仁美, 東元 健, 矢田有加里, 遠藤高帆, Sharif Jafar, 小森敏治, , 松田正史, 古関庸子, 中山学, 副島英伸, 半田宏, 古関明彦, 広瀬進, 西岡憲一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-03-06
• [Presentation] Beckwith-Wiedemann 症候群と関連疾患におけるゲノム・エピゲノム異常 (2013)
  • Author(s): 副島英伸
  • Organizer: 日本人類遺伝学会第58回大会「シンポジウム8 先天異常とゲノム・エピゲノム」
  • Place of Presentation: 仙台
• [Presentation] シスチン尿症を伴うゲノムワイド父性片親性ダイソミー症例の遺伝子解析 (2013)
  • Author(s): 大塚泰史, 佐々木健作, 城崎幸介, 東元健, 岡本信彦, 高間勇一, 窪田昭男, 松本富美, 中山雅弘, 吉浦孝一郎, 副島英伸
  • Organizer: 日本人類遺伝学会第58回大会
  • Place of Presentation: 仙台
• [Presentation] H19DMR メチル化異常で発症するインプリント疾患におけるH19DMR の変異解析 (2013)
  • Author(s): 東元 健, 城崎幸介, 八木ひとみ, 古庄知己, 松原圭子, 山田大輔, 前田寿幸, 大塚泰史, 古関明彦, 緒方勤, 副島英伸
  • Organizer: 第7回日本エピジェネティクス研究会年会
  • Place of Presentation: 奈良
• [Presentation] Beckwith-Wiedemann 症候群におけるインプリントDMR のマルチローカスメチル化解析 (2013)
  • Author(s): 前田寿幸, 東元健, 中林一彦, 城崎幸介, 八木ひとみ, 緒方勤, 秦健一郎, 副島英伸
  • Organizer: 第7回日本エピジェネティクス研究会年会
  • Place of Presentation: 奈良
• [Presentation] インプリンティング疾患Beckwith-Wiedemann症候群と類縁疾患におけるエピゲノム・ゲノム異常 (2013)
  • Author(s): 副島英伸
  • Organizer: 第5回金沢大学学際科学実験センターシンポジウム「深遠なる疾患エピジェネティクス」
  • Place of Presentation: 金沢大学
  • Invited
• [Presentation] ゲノム・インプリンティングとヒト疾患―間葉性異形成胎盤の分子遺伝学的解析 (2013)
  • Author(s): 副島英伸
  • Organizer: 群馬大学生体調節研究所内分泌・代謝学共同研究拠点セミナー
  • Place of Presentation: 群馬大学
  • Invited
• [Presentation] 新生児期低血糖合併Beckwith-Wiedemann症候群患者における病態形成機序の検討 (2013)
  • Author(s): 長嶋一昭、田中大祐、東元 健、八木ひとみ、杉崎 和、田原 裕美子、小倉かさね、佐藤広規、佐藤雄一、山野 言、副島英伸、稲垣暢也
  • Organizer: 第56回日本糖尿病学会年次学術集会
  • Place of Presentation: 熊本
• [Presentation] Beckwith-Wiedemann症候群におけるインプリントDMRのマルチローカスメチル化解析 (2013)
  • Author(s): 前田寿幸、東元健、中林一彦、城崎幸介、八木ひとみ、緒方勤、秦健一郎、副島英伸
  • Organizer: 第7回日本エピジェネティクス研究会年会
  • Place of Presentation: 奈良
• [Presentation] H19DMRメチル化異常で発症するインプリント疾患におけるH19DMRの変異解析 (2013)
  • Author(s): 東元 健、城崎幸介、八木ひとみ、古庄知己、松原圭子、山田大輔、前田寿幸、大塚泰史、古関明彦、緒方勤、副島英伸
  • Organizer: 第7回日本エピジェネティクス研究会年会
  • Place of Presentation: 奈良
• [Presentation] シスチン尿症を伴うゲノムワイド父性片親性ダイソミー症例の遺伝子解析 (2013)
  • Author(s): 大塚泰史、佐々木健作、城崎幸介、東元健、岡本信彦、高間勇一、窪田昭男、松本富美、中山雅弘、吉浦孝一郎、副島英伸
  • Organizer: 第48回日本小児腎臓病学会
  • Place of Presentation: 徳島
• [Presentation] Practical use of pyrosequencing analysis to detect Moyamoya disease susceptible gene RNF213 variant c.14576G>A (2013)
  • Author(s): Takamatsu Y, Maeda T, Matsuo M, Higashimoto K, Kawashima M, Matsushima T, Soejima H
  • Organizer: 3rd International Moyamoya Meeting
  • Place of Presentation: 札幌
• [Presentation] 11p15インプリントドメインのメチル化異常を認めた間葉性異形成胎盤の1例 (2013)
  • Author(s): 副島英伸、東元健、八木ひとみ、青木早織、鮫島梓、齋藤滋、夫律子、中山雅弘、坂口勲、大場隆、片渕秀隆
  • Organizer: 第20回遺伝性疾患に関する出生前診断研究会
  • Place of Presentation: 鹿児島
• [Presentation] 母体血中sFlt-1が異常高値を示したPlacental Mesenchymal Dysplasiaの一例 (2013)
  • Author(s): 鮫島梓、米田徳子、森尻昌人、米澤理可、米田哲、塩崎有宏、夫律子、中山雅弘、副島英伸、齋藤滋
  • Organizer: 第21回日本胎盤学会学術集会
  • Place of Presentation: 名古屋市
• [Presentation] 本邦における間葉性異形成胎盤の臨床像 (2013)
  • Author(s): 青木早織、大場隆、岡島翠、坂口 勲、東元健、副島英伸、福永真治、片渕秀隆
  • Organizer: 第21回日本胎盤学会学術集会
  • Place of Presentation: 名古屋市
• [Presentation] 間葉性異形成胎盤における11p15インプリント領域の分子遺伝学的解析 (2013)
  • Author(s): 副島英伸、東元健、城﨑幸介、八木ひとみ、大塚泰史、前田寿幸、青木早織、岡島翠、坂口勲、大場隆、片渕秀隆
  • Organizer: 第21回日本胎盤学会学術集会
  • Place of Presentation: 名古屋市
• [Presentation] モヤモヤ病原因遺伝子RNF213の琉球諸島・北部九州ヒト集団における遺伝的多型 (2013)
  • Author(s): 小金渕佳江、中込滋樹、間野修平、石崎直也、河村正二、木村亮介、石田肇、城圭一郎、副島英伸、藤本一真、佐藤公俊、湯澤泉、安井美江、隈部俊宏、藤井清孝、秋山辰穂、埴原恒彦、太田博樹
  • Organizer: 第67回日本人類学会大会
  • Place of Presentation: 筑波
• [Presentation] 時計遺伝子 PERIOD2 の多型と光刺激応答の生理的多様性の関係及びその人類学的考察 (2013)
  • Author(s): 秋山辰穂、勝村啓史、埴原恒彦、太田博樹、中込滋樹、藤本一真、副島英伸、城圭一郎、木村亮介、石田肇、安河内朗、樋口重和
  • Organizer: 第67回日本人類学会大会
  • Place of Presentation: 筑波
• [Presentation] 経過観察中にWilms腫瘍を発症した父性ダイソミー型Beckwith-Wiedemann症候群の1例 (2013)
  • Author(s): 中山真悠子、岡陽一郎、近藤剛、村守克己、岩田はるか、城賀本敏宏、石田也寸志、東元健，副島英伸
  • Organizer: 第88回日本小児外科学会愛媛地方会
  • Place of Presentation: 愛媛
• [Presentation] 胎児発育異常症例の網羅的ゲノム・エピゲノム解析 (2013)
  • Author(s): 佐々木かりん、右田王介、中林一彦、東元健、前田寿幸、橋本和法、松井英雄、副島英伸、高田史男、秦健一郎
  • Organizer: 日本人類遺伝学会第58回大会
  • Place of Presentation: 仙台
• [Presentation] 間葉性異形成胎盤における11p15刷り込み領域の分子遺伝学的解析 (2013)
  • Author(s): 副島英伸、東元健、城﨑幸介、八木ひとみ、大塚泰史、前田寿幸、青木早織、岡島翠、坂口勲、大場隆、片渕秀隆
  • Organizer: 日本人類遺伝学会第58回大会
  • Place of Presentation: 仙台
• [Presentation] ゲノムワイドDNAメチル化解析によるヒトインプリントーム解明 (2013)
  • Author(s): 中林一彦、Trujillo Alex Martin、田山千春、兼城英輔、和氣徳夫、副島英伸、緒方勤、Monk David、秦健一郎
  • Organizer: 日本人類遺伝学会第58回大会
  • Place of Presentation: 仙台
• [Presentation] Beckwith-Wiedemann症候群と関連疾患におけるゲノム・エピゲノム異常 (2013)
  • Author(s): 副島英伸
  • Organizer: 日本人類遺伝学会第58回大会
  • Place of Presentation: 仙台
• [Presentation] H19-DMR メチル化異常で発症するインプリント疾患におけるH19-DMR の変異解析 (2012)
  • Author(s): 東元 健, 城崎幸介, 八木ひとみ, 古庄知己, 松原圭子, 山田大輔, 前田寿幸, 大塚泰史, 古関明彦, 緒方勤, 副島英伸
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Genome-wide Screening of Aberrant Methylations of Imprinted DMRs in Hepatoblastomas (2012)
  • Author(s): Rumbajan JM, Maeda T, Tajiri T, Higashimoto K, Souzaki R, Taguchi T, Soejima H, Joh K
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Genome-wide quantitative DNA methylation analysis of imprinted DMRs in patients with Beckwith-Wiedemann Syndrome by MALDI-TOF MS technology (2012)
  • Author(s): Maeda T, Jozaki K, Yatsuki H, Higashimoto K, Soejima H
  • Organizer: The American Society of Human Genetics 62nd Annual Meeting (poster 3432T)
  • Place of Presentation: San Francisco, California, USA
• [Presentation] The relationship between paternal uniparental disomy and clinical features in patients with Beckwith-Wiedemann syndrome (2012)
  • Author(s): Ohtsuka Y, Jozaki K, Maeda T, Yatsuki H, Higashimoto K, Soejima H
  • Organizer: The American Society of Human Genetics 62nd Annual Meeting (poster 3519T)
  • Place of Presentation: San Francisco, California, USA
• [Presentation] Beckwith-Wiedemann 症候群における片親性父性ダイソミーの多様性と臨床症状との関連 (2012)
  • Author(s): 大塚泰史, 城崎幸介, 前田寿幸, 八木ひとみ, 東元 健, 副島英伸
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 東京
• [Presentation] 肝芽腫におけるメチル化インプリントDMR のゲノムワイド検索 (2012)
  • Author(s): 副島英伸, 東元 健, 田尻達郎
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市
• [Presentation] Beckwith-Wiedemann症候群における片親性父性ダイソミーの多様性と臨床症状との関連 (2012)
  • Author(s): 大塚泰史
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
• [Presentation] Beckwith-Wiedemann症候群エピ変異症例のゲノム網羅的DNAメチル化解析 (2012)
  • Author(s): 前田寿幸
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
• [Presentation] 全ゲノム片親性ダイソミー症例のDNAメチル化解析によるヒトインプリント遺伝子座位の網羅的解析 (2012)
  • Author(s): 田山千春
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
• [Presentation] 肝芽腫におけるインプリントDMR異常メチル化のゲノムワイド検索 (2012)
  • Author(s): Janette M. Rumbajan
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
• [Presentation] Beckwith-Wiedemann症候群の遺伝カウンセリング (2012)
  • Author(s): 大町和美
  • Organizer: 第36回日本遺伝カウンセリング学会学術集会
  • Place of Presentation: 信州大学
• [Presentation] 肝芽腫におけるメチル化インプリントDMRのゲノムワイド検索 (2012)
  • Author(s): 副島英伸
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市
• [Presentation] パイロシークエンス法を用いたもやもや病感受性遺伝子RNF213のc.14576G>A多型解析 (2012)
  • Author(s): 髙松裕一郎
  • Organizer: 日本脳神経外科学会第71回学術総会
  • Place of Presentation: 大阪
• [Presentation] Perlman症候群におけるDIS3L2のエクソン9の欠失はLINE-1間の非相同組換えによって生じる (2012)
  • Author(s): 東元 健
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 東京
• [Presentation] 19-DMRにメチル化異常を認めたインプリント疾患におけるH19-DMRの変異解析 (2012)
  • Author(s): 東元 健
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 東京
• [Presentation] Beckwith-Wiedemann症候群エピ変異症例におけるインプリントDMRの網羅的メチル化解析 (2012)
  • Author(s): 前田寿幸
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 東京
• [Presentation] Beckwith-Wiedemann症候群における片親性父性ダイソミーの多様性と臨床症状との関連 (2012)
  • Author(s): 大塚泰史
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 東京
• [Presentation] 全ゲノム片親性ダイソミー症例のDNAメチル化解析によるヒトインプリントーム解明 (2012)
  • Author(s): 中林一彦
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 東京
• [Presentation] 肝芽腫におけるインプリントDMRメチル化異常のゲノムワイド検索 (2012)
  • Author(s): 副島英伸
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 東京
• [Presentation] The relationship between paternal uniparental disomy and clinical features in patients with Beckwith-Wiedemann syndrome (2012)
  • Author(s): Ohtsuka Y
  • Organizer: The American Society of Human Genetics 62nd Annual Meeting
  • Place of Presentation: San Francisco, California, USA
• [Presentation] DNA methylation analysis of reciprocal genome-wide UPDs to define imprinted differentially methylated regions in the human genome (2012)
  • Author(s): Nakabayashi K
  • Organizer: The American Society of Human Genetics 62nd Annual Meeting
  • Place of Presentation: San Francisco, California, USA
• [Presentation] Genome-wide quantitative DNA methylation analysis of imprinted DMRs in patients with Beckwith-Wiedemann Syndrome by MALDI-TOF MS technology (2012)
  • Author(s): Maeda T
  • Organizer: The American Society of Human Genetics 62nd Annual Meeting
  • Place of Presentation: San Francisco, California, USA
• [Presentation] H19-DMRメチル化異常で発症するインプリント疾患におけるH19-DMRの変異解析 (2012)
  • Author(s): 東元 健
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 全ゲノム片親性ダイソミー症例のDNAメチル化解析によるヒトインプリントーム解明 (2012)
  • Author(s): 田山千春
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 本邦Beckwith-Wiedemann 症候群の臨床像とゲノム・エピゲノム解析.180 超例の解析により明らかとなった本邦Beckwith-Wiedemann 症候群の臨床像とゲノム・エピゲノム変異 (2011)
  • Author(s): 副島英伸, 城崎幸介, 八木ひとみ, 前田寿幸, 大塚泰史, 東元 健
  • Organizer: 日本人類遺伝学会第56回大会 第11回東アジア人類遺伝学会
  • Place of Presentation: 幕張
• [Presentation] Acquisition of aberrant hypermethylation after implantation induces discordant hypermethylation at H19-DMR between bodies and placentas in Beckwith-Wiedemann syndrome patients (2011)
  • Author(s): Soejima H, Nakabayashi K, Yatsuki H, Jozaki K, Hata K, Higashimoto K
  • Organizer: Idibell Cancer Conferences on Imprinting and Beyond; "Mono-allelic expression in Health and Disease"
  • Place of Presentation: Barcelona, Spain
• [Presentation] Beckwith-Wiedemann症候群の臨床像と遺伝子解析 (2011)
  • Author(s): 副島英伸
  • Organizer: BWS親の会勉強会(招待講演)
  • Place of Presentation: 旭川市
• [Presentation] Beckwith-Wiedemann症候群の臨床像と遺伝子解析 (2011)
  • Author(s): 副島英伸
  • Organizer: BWS親の会勉強会(招待講演)
  • Place of Presentation: 鹿児島市
• [Presentation] Clinical features and genome/epigenome analyses of (2011)
  • Author(s): Soejima H
  • Organizer: The 9th Korean PWS Symposium(招待講演)
  • Place of Presentation: Seoul, Korea
• [Presentation] Methylation screening of reciprocal genome-wide (2011)
  • Author(s): Tayama C, et al.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Role of Histone H3 Lys36 methylation by Ash1l (2011)
  • Author(s): Higashimoto K, et al.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Aberrant methylation of H19-DMR acquired after (2011)
  • Author(s): Soejima H, et al.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 本邦Beckwith-Wiedemann症候群の臨床像とゲノム・エピゲノム解析 (2011)
  • Author(s): 副島英伸、他
  • Organizer: 日本人類遺伝学会第56回大会 第11回東アジア人類遺伝学会
  • Place of Presentation: 幕張
• [Presentation] Hemihypertrophyにおける11番染色体短腕BWS領域の異常について (2011)
  • Author(s): 蒔田芳男、副島英伸
  • Organizer: 日本人類遺伝学会第56回大会 第11回東アジア人類遺伝学会
  • Place of Presentation: 幕張
• [Presentation] 本邦における間葉性異形成胎盤の臨床像 (2011)
  • Author(s): 岡島 翠、大場 隆、片渕秀隆、東元 健、副島英伸
  • Organizer: 第18回遺伝性疾患に関する出生前診断研究会
  • Place of Presentation: 佐賀
• [Presentation] Acquisition of aberrant hypermethylation after (2011)
  • Author(s): Soejima H, et al.
  • Organizer: Idibell Cancer Conferences
  • Place of Presentation: Barcelona, Spain
• [Presentation] p57KIP2（KIP2）の遺伝子変異を認めたBeckwith-Wiedermann症候群 (2011)
  • Author(s): 岡田純一郎、他
  • Organizer: 第34回日本小児遺伝学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Beckwith-Wiedemann症候群に間葉性異形成胎盤を合併し、 (2011)
  • Author(s): 三好潤也、他
  • Organizer: 第19回日本胎盤学会学術集会
  • Place of Presentation: 東京
• [Presentation] 本邦Beckwith-Wiedemann症候群の臨床像とゲノム・エピゲノム解析 (2011)
  • Author(s): 副島英伸、他
  • Organizer: 遺伝医学合同学術集会2011
  • Place of Presentation: 京都
• [Presentation] Beckwith-Wiedemann症候群に間葉性異形成胎盤を合併し、 (2011)
  • Author(s): 三好潤也、他
  • Organizer: 遺伝医学合同学術集会2011
  • Place of Presentation: 京都
• [Presentation] ゲノム・エピゲノム解析による間葉性異形成胎盤の原因遺伝子探索の現状
  • Author(s): 副島英伸
  • Organizer: 熊本大学医学部産婦人科セミナー
  • Place of Presentation: 熊本
  • Invited
• [Presentation] ゲノムインプリンティングとヒト疾患―ベックウィズ・ビーデマン症候群と間葉性異形成胎盤の解析を中心に
  • Author(s): 副島英伸
  • Organizer: 第８回遺伝医学セミナー
  • Place of Presentation: 別府
  • Invited
• [Presentation] Silver－Russell症候群の遺伝学的異常の検討
  • Author(s): 外木秀文
  • Organizer: 第35回小児遺伝学会学術集会
  • Place of Presentation: 久留米市
• [Book] Beckwith-Wiedemann症候群と小児腫瘍.遺伝子医学MOOK25エピジェネティクスと病気 第2 章エピジェネティクスと病気 4.不妊・先天異常(監修:佐々木裕之, 編集:中尾光善, 中島欽一) (2013)
  • Author(s): 東元健, 副島英伸
  • Publisher: メディカルドゥ, 大阪
• [Book] 遺伝子医学MOOK25 (2013)
  • Author(s): 東元健、副島英伸
  • Total Pages: 280
  • Publisher: メディカルドゥ
• [Book] ベックウィズ・ヴィーデマン症候群 第XIV 章 先天異常・奇形 症候群ハンドブック.(井村裕夫総編集, 福井次矢・辻省次編集.) (2011)
  • Author(s): 副島英伸
  • Total Pages: 679
  • Publisher: 中山書店
• [Book] シルバー・ラッセル症候群第XIV 章 先天異常・奇形 症候群ハンドブック.(井村裕夫総編集, 福井次矢・辻省次編集) (2011)
  • Author(s): 副島英伸
  • Total Pages: 685
  • Publisher: 中山書店
• [Book] 症候群ハンドブック、第XIV章先天異常・奇形、ベックウィズ・ヴィーデマン症候群 (2011)
  • Author(s): 副島英伸
  • Total Pages: 757
  • Publisher: 中山書店
• [Book] 症候群ハンドブック、第XIV章先天異常・奇形、シルバー・ラッセル症候群 (2011)
  • Author(s): 副島英伸
  • Total Pages: 757
  • Publisher: 中山書店
• [Remarks] ホームページ
  • URL: http://www.biomol.med.saga-u.ac.jp/mbg/index.htm
• [Remarks] 佐賀大学医学部分子生物学講座分子遺伝学・エピジェネティクス分野
  • URL: http://www.biomol.med.saga-u.ac.jp/mbg/index.htm
• [Remarks] 佐賀大学医学部分子生命科学講座分子遺伝学・エピジェネティクス分野
  • URL: http://www.biomol.med.saga-u.ac.jp/mbg/index.htm
• [Remarks]
  • URL: http://www.biomol.med.saga-u.ac.jp/mbg/index.htm