| Project/Area Number |
23659185
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HAGA Hironori 京都大学, 医学研究科, 教授 (10252462)
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 肺癌 / 質量分析 / 組織マイクロアレイ / 癌 |
|---|
| Research Abstract |
A novel chemical treatment technique for mass spectrometry using formaldehyde fixed paraffin embedded specimen of human cancer tissue was developed. By this technique including swelling process and heating under high pressure in a reaction solution containing acetonitrile, peptide ionization was significantly enhanced. As a result, detection sensitivity for Matrix-assisted laser ionization time-of-flight mass spectrometry (MALDI-TOF MS) was augmented more than 100-fold. We were successful in identification of a tumor marker H2A protein in cancer tissue in comparison with the normal tissue.
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