Development of a new in situ technique demonstrating methyaled cytosine by iso-thermal DNA amplification method
Project/Area Number |
23659186
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Human pathology
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Research Institution | Ehime University |
Principal Investigator |
KITAZAWA Sohei 愛媛大学, 医学(系)研究科(研究院), 教授 (90186239)
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Co-Investigator(Kenkyū-buntansha) |
KITAZAWA Riko 愛媛大学, 大学院・医学系研究科, 准教授 (00273780)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | DNA / メチル化シトシン / 組織細胞化学 / 遺伝子増幅 / in situ hybridization / エピジェネティクス / メチル化 / 組織化学 / PCR / 減数分裂 |
Research Abstract |
By making a ICON probe which specifically bound to methylated cytosine on chromosomal DNA in a sequence specific manner, a strong chemical cross-linking was made by osmium reaction. By so-called PRINS method, a DNA elongation reaction was established using bounded ICON probe on the specific chromosome. Modified nucleotides were incorporated during this elongation stem and visualized by a conventional histochemical techniques. By this easy, methyalation status of specific chromosome or DNA sequence could be visualized. So far we gathered a lot of basic and fundamental data. We are now currently trying to visualize a single-copy gene during spermatogenesis in mouse testis, and updating our protocol for more stable and reliable one.
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] CIZ/NMP4 is expressed in B16 melanoma and forms a positive feedback loop with RANKL to promote migration of the melanoma cells2012
Author(s)
Sakuma, T., Nakamoto, T., Hemmi, H., Kitazawa, S., Kitazawa, R., Notomi, T., Hayata, T., Ezura, Y., Amagasa, T., and Noda, M
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Journal Title
Journal of cellular physiology
Volume: 227
Issue: 7
Pages: 2807-2812
DOI
Related Report
Peer Reviewed
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[Journal Article] Nonalcoholic fatty liver disease in adult hypopituitary patients with growth hormone deficiency and the impact of growth hormone replacement therapy2012
Author(s)
Hitoshi Nishizawa, Genzo Iguchi, Ayumi Murawaki, Hidenori Fukuoka, Yoshitake Hayashi, Hidesuke Kaji, Masaaki Yamamoto, Kentaro Suda, Michiko Takahashi, Yasushi Seo, Yoshihiko Yano, Riko Kitazawa, Sohei Kitazawa, Masafumi Koga, Yasuhiko Okimura, Kazuo Chihara, Yutaka Takahashi
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Journal Title
Eur J Endcri
Volume: 167(1)
Issue: 1
Pages: 67-74
DOI
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Peer Reviewed
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[Journal Article] Pyruvate dehydrogenase kinase 4 induces bone loss at unloading by promoting osteoclastogenesis2012
Author(s)
Wang Y, Liu1 W, Masuyama R, Fukuyama R, Ito M, Zhang Q, Komori H, Murakami T, Moriishi T, Miyazaki T, Kitazawa R, Yoshida CA, Kawai Y, Izumi S, Komori T
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Journal Title
Bone
Volume: 50(1)
Issue: 1
Pages: 9-419
DOI
Related Report
Peer Reviewed
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[Journal Article] PGRN is a key adipokine mediating high fat diet-induced insulin resistance and obesity through IL-6 in adipose tissue.2012
Author(s)
Matsubara T, Mita A, Minami K, Hosooka T, Kitazawa S, Takahashi K, Tamori Y, Yokoi N, Watanabe M, Matsuo E, Nishimura O, Seino S
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Journal Title
Cell Metab
Volume: 15
Pages: 38-50
Related Report
Peer Reviewed
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[Journal Article] Parathyroid hormone-responsive Smad3-related factor, Tmem119, promotes osteoblast differentiation and interacts with the bone morphogenetic protein-Runx2 pathway2011
Author(s)
Hisa I, Inoue Y, Hendy GN, Canaff L, Kitazawa R, Kitazawa S, Komori T, Sugimoto T, Seino S, Kaji H
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Journal Title
J Biol Chem
Volume: 286
Issue: 11
Pages: 9787-9796
DOI
Related Report
Peer Reviewed
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[Journal Article] Carvedilol ameliorates low-turnover bone disease in non-obese type 2 diabetes2011
Author(s)
Shunsuke Goto, Hideki Fujii, Keiji Kono, Kentaro Nakai, Yasuhiro Hamada, Hideyuki Yamato, Masami Shinohara, Riko Kitazawa, Sohei Kitazawa, Shinichi Nishi, and Masafumi Fukagaw
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Journal Title
Am J Nephrol
Volume: 34(3)
Issue: 3
Pages: 281-290
DOI
Related Report
Peer Reviewed
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[Journal Article] Chemerin regulates β-cell function in mice.2011
Author(s)
Takahashi M, Okimura Y, Iguchi G, Nishizawa H, Yamamoto M, Suda K, Kitazawa R, Fujimoto W, Takahashi K, Zolotaryov FN, Hong KS, Kiyonari H, Abe T, Kaji H, Kitazawa S, Kasuga M, Chihara K, Takahashi Y.
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Journal Title
Scientific Reports
Volume: 1
Issue: 1
Pages: 123-123
DOI
Related Report
Peer Reviewed
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[Journal Article] Carvedilol ameliorates low-turnover bone disease in non-obese type 2 diabetes.2011
Author(s)
Goto S, Fujii H, Kono K, Nakai K, Hamada Y, Yamato H, Shinohara M, Kitazawa R, Kitazawa S, Nishi S, Fukagawa M
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Journal Title
Am J Nephrol
Volume: 34
Pages: 281-290
Related Report
Peer Reviewed
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