Novel therapy of myxoid liposarcoma by Chromosome re-programming
| Project/Area Number |
23659192
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脂肪肉腫 / 染色体転座 / TLS-CHOP 遺伝子 / iPS 細胞 / 粘液型脂肪肉腫 / TLS-CHOP / 染色体リプログラミング / miRNA / TLS/FUS-CHOP遺伝子 / iPS細胞 |
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| Research Abstract |
Myxoid liposarcomas (MLSs) are characterized by t(12;16)(q13;p11) translocation and expression of TLS-CHOP chimeric oncoprotein. In this study, we have found that miR-486 regulate differentiation of adipocyte. Furthermore, miR-486 expression was repressed in TLS-CHOP-expressed NIH3T3 fibroblasts and MLS tissues, and exogenous overexpression of miR-486 repressed growth of MLS cells. In addition, we have identified plasminogen activator inhibitor-1 (PAI-1) as a novel target gene of miR-486. Furthermore, knockdown of PAI-1 by a specific small interfering RNA (siRNA) inhibited growth of MLS cells, suggesting that increased expression of PAI-1 by miR-486 repression is critical for survival of MLS cells. Collectively, these results suggest a novel essential molecular mechanism that TLS-CHOP activates PAI-1 expression by repression of miR-486 expression in MLS tumorigenesis and development.
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Report
(3 results)
Research Products
(96 results)
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[Journal Article] SMAD2 MEDIATES DENDRITIC CELL SUPPRESSION BY TGF-β Smad2 is the TGF-β receptor-regulated Smad to suppress the immunogenicity of mouse and human dendritic cells2013
Author(s)
Yoon JH, Sudo K, Han JS, Chae MY, Park SH, Weinstein M, Nakae S, Yamashita T, Lee IK, Sumida T, Kuroda M, and Mamura M
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Journal Title
European Journal of Immunology -2
Volume: Manuscript ID: eji.201242972.R1
Related Report
Peer Reviewed
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[Journal Article] GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance2013
Author(s)
Nakaya M, Tajima M, Kosako H, Nakaya T, Hashimoto A, Watari K, Nishihara H, Ohba M, Komiya S, Tani N, Nishida M, Taniguchi H, Sato Y, Matsumoto M, Tsuda M, Kuroda M, Inoue K, Kurose H
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Journal Title
Nature Commun
Volume: 4
Issue: 1
Pages: 1532-1532
DOI
Related Report
Peer Reviewed
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[Journal Article] The expression level of miR-18b in hepatocellular carcinoma is associated with the grade of malignancy and prognosis2013
Author(s)
Murakami Y, Tamori A, Itami S, Tanahashi T, Toyoda H, Tanaka M, Wu W, Brojigin N, Kaneoka Y, Maeda A, Kumada T, Kawada N, Kubo S and Kuroda M.
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Journal Title
BMC Cancer
Volume: 13
Issue: 1
Pages: 99-99
DOI
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Peer Reviewed
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[Journal Article] TLS-CHOP represses miR-486 expression, inducing upregulation of a metastasis regulator PAI-1 in human myxoid liposarcoma2012
Author(s)
N. Borjigin, S. Ohno, W. Wu, M. Tanaka, R. Suzuki, K. Fujita, M. Takanashi, K. Oikawa, T. Goto, T. Motoi, T. Kosaka, K. Yamamoto, M. Kuroda
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 427
Issue: 2
Pages: 355-360
DOI
Related Report
Peer Reviewed
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[Journal Article] Efficacy of a novel class of RNA interference therapeutic agents2012
Author(s)
Hamasaki T, Suzuki H, Shirohzu H, Matsumoto T, D'Alessandro-Gabazza CN,Gil-Bernabe P, Boveda-Ruiz D, Naito M, Kobayashi T, Toda M, Mizutani T, TaguchiO, Morser J, Eguchi Y, Kuroda M, Ochiya T, Hayashi H, Gabazza EC, Ohgi T
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Journal Title
PLoS One
Volume: 7
Issue: 8
Pages: 1-15
DOI
Related Report
Peer Reviewed
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[Journal Article] Regulation of the development of acute hepatitis by IL-23 through IL-22 and IL-17 production.2011
Author(s)
Xu M, Morishima N, Mizoguchi I, Chiba Y, Fujita K, Kuroda M, Iwakura Y, Cua DJ, Yasutomo K, Mizuguchi J, Yoshimoto T.
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Journal Title
Eur J Immunol.
Volume: 41
Pages: 2828-2839
Related Report
Peer Reviewed
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[Journal Article] Dysregulation of IFN system can lead to poor response to pegylated interferon and ribavirin therapy in chronic hepatitis C.2011
Author(s)
Onomoto K, Morimoto S, Kawaguchi T, Toyoda H, Tanaka M, Kuroda M, Uno K, Kumada T, Matsuda F, Shimotohno K, Fujita T, Murakami Y.
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Journal Title
NAID
Related Report
Peer Reviewed
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[Presentation] KLF5 is essential for oncogenesis of intestinal tumors and control of intestinal stem cells2012
Author(s)
Nakaya T, Ogawa S, Manabe I, TanakaM, Sanada M, Sato T, Goto M, Taketo M,Nakao K, Fukayama M, Kuroda M, Nagai R
Organizer
第35回分子生物学会年会
Place of Presentation
福岡
Year and Date
2012-12-11
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[Presentation] Regulation of the development of acute hepatitis by IL-23 through IL-22 and IL-17 production2011
Author(s)
Xu M, Morishima N Mizoguchi I, Chiba Y, Fujita K, Kuroda M, Iwakura Y, Cua DJ, Yasutomo K, Mizuguchi J, Yoshimoto T
Organizer
IMMUNOLOGY 2011, 98th Annual Meeting of The American Association of Immunologists
Place of Presentation
San Francisco, California
Year and Date
2011-05-13
Related Report
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[Presentation] The signification of lactoferrin in secondary corneal amyloidosis.2011
Author(s)
Hirano K, Araki-Sasaki K, OsakabeY, Tanaka K, Kuroda M, Fukuoka H, Kawasaki S, Maeda N, Kinoshita S
Organizer
2011 ARVO Annual Meeting
Place of Presentation
Fort Lauderdale, Florida, USA
Year and Date
2011-05-01
Related Report
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[Presentation] The signification of lactoferrin in secondary corneal amyloidosis.2011
Author(s)
Koji Hirano, Kaoru Araki-Sasaki, Yasuhiro Osakabe, Keisuke Tanaka, Masahiko Kuroda, Hideki Fukuoka, Satoshi Kawasaki, Naoyuki Maeda, Shigeru Kinoshita.
Organizer
ARVO Annual Meeting.
Place of Presentation
Fort Lauderdale, Florida, USA.
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