Chimerism of human transplanted liver and possibility of anti-aging effect of chimera hepatocytes

• Project/Area Number: 23659193
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• Principal Investigator: TAKUBO Kaiyo 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究部長 (00154956)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生体肝移植 / 臨床病理学的研究 / テロメア / キメラ / 肝移植 / 移植肝 / キメリズム / FISH / 抗老化

Research Abstract

Based on the analysis on the database of the patients of living-donor liver transplantation (LDLT), it was indicated that maternal grafts protected daughter and son recipients from acute rejection after pediatric LDLT for biliary atresia. It was suggested that younger donors were better for LDLT patients to leave immunosuppressant. Q-FISH measurement of hepatocyte telomere lengths in donor liver and graft after LDLT indicated that donor age was a crucial factor affecting telomere length sustainability in hepatocytes after pediatric LDLT, and that the telomeres in grafts may be elongated somewhat longer when the grafts were immunologically well controlled.In the maternal grafts, many Y chromosome positive cells (mainly, endothelial cells) from son recipients were observed, whereas Y-chromosome and keratin-positive cells were very scanty in the grafts.

Research Products

• [Journal Article] Maternal grafts protect daughter recipients from acute cellular rejection after pediatric living donor liver transplantation for biliary atresia (2014)
  • Author(s): Sanada Y, Kawano Y, Miki A, Aida J, Nakamura K, Izumiyama NS, Ishikawa N, Arai T, Hirata Y, Yamada N, Okada N, Wakiya T, Ihara Y, Urahashi T, Yasuda Y, Takubo K.
  • Journal Title: Transpl Int. Volume: 27 Pages: 383-390
  • DOI: 10.1111/tri.12273
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Q-FISH measurement of hepatocyte telomere lengths in donor liver and graft after pediatric living-donor liver transplantation: donor age affects telomere length sustainability. (2014)
  • Author(s): Kawano Y., Ishikawa N., Aida J., Sanada Y., Izumiyama-Shimomura N., Nakamura K., Poon SSS., Matsumoto K., Mizuta K., Uchida E., Tajiri T., Kawarasaki H., Takubo K.
  • Journal Title: PLoS One Volume: 9 Pages: 1-10
  • DOI: 10.1371/journal.pone.0093749
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hepatocellular Telomere Length in Biliary Atresia Measured by Q-FISH (2014)
  • Author(s): Sanada Y, Aida J, Kawano Y, Nakamura KI, Shimomura N, Ishikawa N, Arai T, Poon SS, Yamada N, Okada N, Wakiya T, Hayashida M, Saito T, Egami S, Hishikawa S, Ihara Y, Urahashi T, Mizuta K, Yasuda Y, Kawarasaki H, Takubo K
  • Journal Title: World J Surg Volume: 36 Pages: 908-916
• [Journal Article] Hepatocellular telomere length in biliary atresia measured by Q-FISH. (2012)
  • Author(s): Sanada Y, Aida J, Kawano Y, Nakamura KI, Shimomura N, Ishikawa N, Arai T, Poon SS, Yamada N, Okada N, Wakiya T, Hayashida M, Saito T, Egami S, Hishikawa S, Ihara Y, Urahashi T, Mizuta K, Yasuda Y, Kawarasaki H, Takubo K.
  • Journal Title: World J Surg Volume: 36 Pages: 908-16
  • DOI: 10.1007/s00268-012-1453-z
  • Peer Reviewed
• [Presentation] Q-FI SH法により測定したテロメア長を指標とした超百歳リンパ球から樹立されたiPS細胞の評価 (2013)
  • Author(s): 仲村賢一, 広瀬信行, 下村-泉山七生貴, 相田順子, 石川直, 田久保海誉
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
• [Presentation] iPS細胞化によるリプログラミング過程でのテロメア動態のQ-FISH法による解析 (2013)
  • Author(s): 石川直, 仲村賢一, 下村-泉山七生貴, 相田順子, 寺井政憲, 豊田雅士, 田久保海誉
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
• [Presentation] Ｑ-ＦＩＳＨ法により測定したテロメア長を指標とした超百歳リンパ球から樹立されたｉＰＳ細胞の評価 (2013)
  • Author(s): 仲村賢一、広瀬信行、下村-泉山七生貴、相田順子、石川直、田久保海誉
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
• [Presentation] ｉＰＳ細胞化によるリプログラミング過程でのテロメア動態のＱ-ＦＩＳＨ法による解析 (2013)
  • Author(s): 石川直、仲村賢一、下村-泉山七生貴、相田順子、寺井政憲、豊田雅士、田久保海誉
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
• [Remarks] 東京都健康長寿医療センター研究所老年病理学研究チーム 高齢者がん研究グループ独自ホームページ
  • URL: http://www.ttaggg-rtgp.org/
• [Remarks] 東京都健康長寿医療センター研究所 老年病理学研究チーム 高齢者がんグループ ホームページ
  • URL: http://www.ttaggg-rtgp.org/
• [Remarks] 東京都健康長寿医療センター研究所老年病理学研究チーム高齢者がん研究グループ
  • URL: http://www.ttaggg-rtgp.org/
• [Remarks] グループのホームページURL：
  • URL: http://www.ttaggg-rtgp.org/