| Project/Area Number |
23659194
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | 愛知県がんセンター(研究所) |
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Principal Investigator |
SETO Masao 愛知県がんセンター(研究所), 遺伝子医療研究部, 部長 (80154665)
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| Project Period (FY) |
2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 血液 / 悪性リンパ腫 / NK細胞 / ゲノム異常 |
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| Research Abstract |
In an attempt to clarify molecular mechanisms of NK cell tumor development, oligo-array CGH and expression profiling were applied. Oligo-array CGH identified two distinct minimal common deleted regions at 6q21 where the most frequent deletion was observed at the percentage of 36%(14 of 39 cases). The genes identified were PRDM1 and FOXO3 which were functionally approved by inducing expression system. Normal NK cells were know to be expanded by culturing peripheral blood mononuclear cells(PBMC) with irradiated K562-mb15-41BBL cells to one million order of magnitude. We could amplified normal NK cells to the same order of magnitude with feeder cells recovered from frozen status. This system will be useful for the functional analysis of genes involved in NK cell tumor development.
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