Development of a novel MRI contrast medium toward detection of hypoxic lesions and quantification of partial oxygen pressure

• Project/Area Number: 23659197
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Tohoku University
• Principal Investigator: FUKUMOTO Manabu 東北大学, 加齢医学研究所, 教授 (60156809)
• Co-Investigator(Kenkyū-buntansha): TAKAMI Seiichi 東北大学, 多元物質科学研究所, 准教授 (40311550) ; KAWAKAMI Kazuyoshi 東北大学, 医学系研究科, 教授 (10253973) ; ABE Keietsu 東北大学, 農学研究科, 教授 (50312624)
• Project Period (FY): 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 低酸素 / ナノ粒子 / MRI / がん幹細胞 / がん治療

Research Abstract

In order to eradicate cancer, we need establish diagnostic and therapeutic methods specially targeting hypoxic lesions. We intended to develop contrast medium for MRI composed of oxide iron nanoparticles which can seek for hypoxic lesions. In vitro study that these nanoparticles were ingested into cancer cells under hypoxic conditions and revealed cytotoxic property. Using transplanted tumors into mice, we will evaluate the in vivo potential of the oxide nanoparticles.

Research Products

• [Journal Article] Activation of the AKT/cyclin D1/Cdk4 survival signaling pathway in radioresistant cancer stem cells (2012)
  • Author(s): Shimura T, Noma N, Oikawa T, Ochiai Y, Kakuda S, Kuwahara Y, Takai Y, Fukumoto M
  • Journal Title: Oncogenesis
• [Journal Article] 麹菌を用いた生分解性プラスチックリサイクルシステム (2012)
  • Author(s): 高橋徹、阿部敬悦
  • Journal Title: 月刊プラスチック Volume: (in press)
• [Journal Article] TLR9-dependent activation of bone marrow-derived dendritic cells by URA5 DNA from Cryptococcus neoformans (2012)
  • Author(s): Tanaka M, Ishii K, Nakamura Y, Miyazato A, Maki A, Abe Y, Miyasaka T, Yamamoto H, Akahori Y, Fue M, Takahashi Y, Kanno E, Maruyama R, Kawakami K
  • Journal Title: Infect. Immun Volume: 80 Pages: 778-786
• [Journal Article] Continuous Hydrothermal Synthesis of 3, 4-Dihydroxyhydrocinnamic Acid-Modified Magnetite Nanoparticles with Stealth-Functionality against Immunological Response (2012)
  • Author(s): T. Togashi, S. Takami, K. Kawakami, H. Yamamoto, T. Naka, K. Sato, K. Abe, T. Adschiri
  • Journal Title: J. Mater. Chem. Volume: 22 Pages: 9041-9045
• [Journal Article] Synthesis of surface-modified monoclinic ZrO2 nanoparticles using supercritical water (2012)
  • Author(s): M. Taguchi, S. Takami, T. Adschiri, T. Nakane, K. Sato, T. Naka
  • Journal Title: CrystEngComm Volume: 14 Pages: 2132-2138
• [Journal Article] Synthesis of shape-controlled organic-hybridized hafnium oxide nanoparticles under sub-and supercritical hydrothermal conditions (2012)
  • Author(s): A. Sahraneshin, S. Takami, D. Hojo, K. Minami, T. Arita, T. Adschiri
  • Journal Title: J. Supercrit. Fluids Volume: 62 Pages: 190-196
• [Journal Article] Activation of the AKT/cyclin D1/Cdk4 survival signaling pathway in radioresistant cancer stem cells (2012)
  • Author(s): Shimura T, et al
  • Journal Title: Oncogenesis Volume: (未定)(accepted)
  • Peer Reviewed
• [Journal Article] Targeting the AKT/GSK3./cyclin D1/Cdk4 survival signaling pathway for eradication of tumor radioresistance acquired by fractionated radiotherapy (2011)
  • Author(s): Shimura T, Kakuda S, Ochiai Y, KuwaharaY, Takai Y, Fukumoto M
  • Journal Title: Int J Radiat Oncol Biol Phys Volume: 80(2) Pages: 540-8
• [Journal Article] Activation of the EGFR, AKT and ERK1/2 by exposure to tritiated water in human tumor cells (2011)
  • Author(s): Shimura T, Kuwahara Y, Fukumoto M, Umata T
  • Journal Title: Fusion Sci Technol Volume: 60(3) Pages: 1190-2
• [Journal Article] Neutrophil gelatinase-associated lipocalin : A new antioxidant that exerts its cytoprotective effect independent on Heme Oxygenase-1 (2011)
  • Author(s): Roudkenar MH, Halabian R, Bahmani P, Roushandeh AM, Kuwahara Y, Fukumoto M
  • Journal Title: Free Radic Res Volume: 45(7) Pages: 810-9
• [Journal Article] Enhancement of autophagy is a potential modality for tumors refractory to radiotherapy (2011)
  • Author(s): Kuwahara Y, Oikawa T, Ochiai Y, Roudkenar MH, Fukumoto M, Shimura T, Ohtake Y, Ohkubo Y, Mori S, Uchiyama Y, Fukumoto M
  • Journal Title: Cell Death Dis Volume: 2
• [Journal Article] A narrow microbeam is more effective for tumor growth suppression than a wide microbeam : an in vivo study using implanted human glioma cells (2011)
  • Author(s): Uyama A, Kondoh T, Nariyama N, Umetani K, Fukumoto M, Shinohara K, Kohmura E
  • Journal Title: J Synchrotron Radiat Volume: 18(Pt4) Pages: 671-8
• [Journal Article] Invariant NKT cells recognize glycolipids from pathogenic gram-positive bacteria (2011)
  • Author(s): Kinjo Y, Illarionov P, Pei B, Vela JL, Girardi E, Li X, Li Y, Imamura M, Rogers P, Uchiyama S, Khurana A, Ainge GD, Gibson D, Kawahara K, Nizet V, Yesilkaya V, Andrew P, Wong CH, Painter G, Kawakami K, Besra GS, Tsuji M, Zajonc DM, Kronenberg M
  • Journal Title: Nat. Immunol Volume: 12 Pages: 966-974
• [Journal Article] Cryptococcus neoformans suppresses the activation of bone marrow-derived dendritic cells stimulated with its own DNA, but not with DNA from other fungi (2011)
  • Author(s): Yamamoto H, Abe Y, Miyazato A, Tanno D, Tanaka M, Miyasaka T, Ishii K, Kawakami K
  • Journal Title: FEMS Immunol. Med. Microbiol. Volume: 63 Pages: 363-372
• [Journal Article] 放射線病理学:トロトラスト症から (2011)
  • Author(s): 福本学
  • Journal Title: 病理と臨床 Volume: 29(7) Pages: 761-8
• [Journal Article] X線照射後のがん細胞で見られる様々な細胞死 (2011)
  • Author(s): 桑原義和, 及川利幸, 落合泰史, 福本基, 栗原愛, 野間直十, 大久保恭仁, 志村勉, 福本学
  • Journal Title: 放射線生物 Volume: 46(3) Pages: 271-82
• [Journal Article] Targeting the AKT/GSK3β/cyclin D1/Cdk4 survival signaling pathway for eradication of tumor radioresistance acquired by fractionated radiotherapy (2011)
  • Author(s): Shimura T, et al
  • Journal Title: Int J Radiat Oncol Biol Phys Volume: 80 Pages: 540-8
  • Peer Reviewed
• [Journal Article] Activation of the EGFR, AKT and ERK1/2 by exposure to tritiated water in human tumor cells (2011)
  • Author(s): Shimura T, et al
  • Journal Title: Fusion Sci Technol Volume: 60 Pages: 1190-2
  • Peer Reviewed
• [Journal Article] Neutrophil gelatinase-associated lipocalin : A new antioxidant that exerts its cytoprotective effect independent on Heme Oxygenase-1 (2011)
  • Author(s): Roudkenar MH, et al
  • Journal Title: Free Radic Res Volume: 45 Pages: 810-9
  • Peer Reviewed
• [Journal Article] Enhancement of autophagy is a potential modality for tumors refractory to radiotherapy (2011)
  • Author(s): Kuwahara Y, Oikawa T, Ochiai Y, Roudkenar, M Fukumoto, T Shimura, Y Ohtake M H, Ohkubo Y, Mori S, Uchiyama Y, Fukumoto M
  • Journal Title: Cell Death Dis (2011) Volume: 2 Issue: 6 Pages: e177-e177
  • DOI: 10.1038/cddis.2011.56
  • Peer Reviewed
• [Journal Article] A narrow microbeam is more effective for tumor growth suppression than a wide microbeam : an in vivo study using implanted human glioma cells (2011)
  • Author(s): Uyama A, et al
  • Journal Title: J Synchrotron Radiat Volume: 18 Pages: 671-8
  • Peer Reviewed
• [Journal Article] 放射線病理学:トロトラストト症から (2011)
  • Author(s): 福本学, 他
  • Journal Title: 病理と臨床 Volume: 29 Pages: 761-8
  • Peer Reviewed
• [Journal Article] X線照射後のがん細胞で見られる様々な細胞死 (2011)
  • Author(s): 桑原義和, 他
  • Journal Title: 放射線生物 Volume: 46 Pages: 271-82
  • Peer Reviewed
• [Presentation] 糸状菌の界面活性タンパク質による生分解生プラスチックの新規分解促進機構とその応用 (2012)
  • Author(s): 高橋徹, 阿部敬悦
  • Organizer: 日本化学会92春季年会
  • Place of Presentation: 慶応大学日吉キャンパス
  • Year and Date: 2012-03-28
• [Presentation] 糸状菌由来免疫回避機能性素材を用いた新規医療用ナノ粒子の開発 (2012)
  • Author(s): 高橋徹村垣公英, 川上和義, 冨樫貴成, 高見誠一, 阿尻雅文, 福本学, 阿部敬悦
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都)
• [Presentation] 脳腫瘍の放射線治療に伴う脳壊死-機序と対策- (2011)
  • Author(s): 福本学
  • Organizer: 日本放射線腫瘍学会第24回学術大会日本放射線腫瘍学会第24回学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-17
• [Presentation] 放射線障害の病理学:トロトラスト症から臨床的放射線耐性へ (2011)
  • Author(s): 福本学
  • Organizer: 日本放射線腫瘍学会第24回学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-17
• [Presentation] 脳腫瘍の放射線治療に伴う脳壊死-機序と対策- (2011)
  • Author(s): 福本学
  • Organizer: 日本放射線腫瘍学会第24回学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-17
• [Presentation] 放射線障害の病理学:トロトラスト症から臨床的放射線耐性へ (2011)
  • Author(s): 福本学
  • Organizer: 日本放射線腫瘍学会第24回学術大会
  • Place of Presentation: 神戸(招待講演)
  • Year and Date: 2011-11-17
• [Presentation] 放射線被ばく総論 (2011)
  • Author(s): 福本学
  • Organizer: 母子保健指導者研修会
  • Place of Presentation: 盛岡
  • Year and Date: 2011-09-18
• [Presentation] 放射線被ばく総論 (2011)
  • Author(s): 福本学
  • Organizer: 母子保健指導者研修会
  • Place of Presentation: 盛岡(招待講演)
  • Year and Date: 2011-09-18
• [Presentation] X線蛍光分析法を用いて考察したトロトラスト沈着肝における代謝と発がんの関係 (2011)
  • Author(s): 福本学
  • Organizer: ワークショップ:量子ビームを用いた物質・生命科学の新展開
  • Place of Presentation: 仙台
• [Presentation] AKT経路を標的としたがん幹細胞の放射線耐性の克服 (2011)
  • Author(s): 志村勉, 他
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸
• [Presentation] 臨床的放射線耐性細胞はなぜドセタキセルに耐性を示すのか? (2011)
  • Author(s): 桑原義和, 他
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸
• [Presentation] Long incubation period of cancer induced by internal exposure is attributed to the uneven distribution of deposited radionuckides at the microscopic level (2011)
  • Author(s): Manabu Fukumoto
  • Organizer: The 6th International Symposium of Nagasaki University Global COE Program"Global Strategic Center for Radiation Health Risk Control"
  • Place of Presentation: 長崎
• [Presentation] Clinically relevant radioresistant cells are resistant to hydrogen peroxide (2011)
  • Author(s): 桑原義和, 他
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] The effect of boron neutron capture therapy targeting tumor endohtelial cells to clinically relevant radioresistant cell (2011)
  • Author(s): 福本基, 他
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Histological type of Thorotrast-induced tumors depends on the metabolic behavio of radionuclides (2011)
  • Author(s): 山本陽一朗, 他
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Clinically Relevant Radioresistant Cells are Established through Acquired Radioresistance by Exposure to Long-term Factionated X-ray Radiation (2011)
  • Author(s): Fukumoto M, et al
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw, Poland
• [Presentation] The e-Pathologists Cancer Diagnosisi Assistance System for Gastric Biopsy Tissues (2011)
  • Author(s): Fukumoto M
  • Organizer: 1st Congress of the International Academy of Digital Pathology
  • Place of Presentation: Quebec City, Canada
• [Presentation] POS36-12. Clinically Relevant Radioresistant Cells are Established through Acquired Radioresistance by Exposure to Long-term Factionated X-ray Radiation (2011)
  • Author(s): Manabu Fukumoto, et al
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw, Poland
• [Presentation] Long incubation period of cancer induced by internal exposure is attributed to the uneven distribution of deposited radionuckides at the microscopic level (2011)
  • Author(s): Manabu Fukumoto
  • Organizer: The 6th International Symposium of Nagasaki University Global COE Program "Global Strategic Center for Radiation Health Risk Control"
  • Place of Presentation: 長崎(招待講演)
• [Book] 放射線被ばくによる発癌:特に内部ひばく発癌機構. 病気の分子形態学 (2011)
  • Author(s): 福本学
  • Publisher: 学際企画
• [Book] 病気の分子形態学 (2011)
  • Author(s): 日本臨床分子形態学会編
  • Publisher: 学際企画