Establishment of Peyer's patch M-cell-deficient mice to analyzeM-cell function

• Project/Area Number: 23659199
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Shintaro 東京大学, 医科学研究所, 助教 (80447333)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 発生病理 / M 細胞 / M細胞 / 細胞分化 / 遺伝子改変マウス / DT-A

Research Abstract

Although many of the biological features of microfold cells (M cells) have been known for many years, the molecular mechanisms of M-cell development and antigen recognition have remained unclear. Here, we report that transcription factor Spi-B was specifically expressed in M cells among non-hematopoetic lineages. Spi-B-deficient mice showed reducedexpression of most, but not all, other M cell-specific genes and M-cell surface markers. Whereas uptake of Salmonella Typhimurium via M cells was obviously reduced in Spi-B-deficient mice, the abundance of intra-tissue cohabiting bacteria was comparable between wild-type and Spi-B-deficient mice. These data indicate that there is a small M-cell population with developmental regulation that is Spi-B independent; however, Spi-B is probably a candidate master regulator of M-cell functional maturation and development by another pathway.

Research Products

• [Journal Article] Nanogel-Based PspA Intranasal Vaccine Prevents Invasive Disease and Nasal Colonization by Streptococcus pneumoniae (2013)
  • Author(s): Il Gyu Kong, Ayuko Sato, Yoshikazu Yuki, Tomonori Nochi, Haruko Takahashi, et al.
  • Journal Title: Infection and Immunity Volume: (online) Issue: 5 Pages: 1625-34
  • DOI: 10.1128/iai.00240-13
  • Peer Reviewed
• [Journal Article] Transcription factor Spi-B-dependent and -independent pathways for the development of Peyer's patch M cells (2012)
  • Author(s): Sato, S., et al.
  • Journal Title: Mucosal Immunology Volume: ６ Issue: 4 Pages: 838-846
  • DOI: 10.1038/mi.2012.122
  • Peer Reviewed
• [Journal Article] Lipocalin 2 bolsters innate and adaptive immune responses to blood-stage malaria infection by reinforcing host iron metabolism. (2012)
  • Author(s): Hong Zhao
  • Journal Title: Cell Host & Microbe Volume: 12 Issue: 5 Pages: 705-716
  • DOI: 10.1016/j.chom.2012.10.010
  • Peer Reviewed
• [Journal Article] Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors (2012)
  • Author(s): Y. Kurashima, T. Amiya, T. Nochi, K. Fujisawa, T. Haraguchi, H. Iba, H. Tsutsui, S. Sato, S. Nakajima, H. Iijima, M. Kubo, J. Kunisawa, H. Kiyono
  • Journal Title: Nat Commun. Volume: 3-1034 Issue: 1 Pages: 1034-1034
  • DOI: 10.1038/ncomms2023
  • Peer Reviewed
• [Journal Article] The Mucosal immune system of the respiratory tract. (2012)
  • Author(s): Sato, S
  • Journal Title: Curr. Opin. Virol. Volume: 2 Issue: 3 Pages: 225-232
  • DOI: 10.1016/j.coviro.2012.03.009
  • Peer Reviewed
• [Journal Article] The airway antigen sampling system: respiratory M cells as an alternative gateway for inhaled antigens (2011)
  • Author(s): Kim DY, Sato A, Fukuyama S, Sagara H, Nagatake T, Kong IG, Goda K, Nochi T, Kunisawa J, Sato S, Yokota Y, Lee CH, Kiyono H
  • Journal Title: J Immunol Volume: 186 Pages: 4253-4262
• [Journal Article] The airway antigen sampling system : respiratory M cells as an alternative gateway for inhaled antigens (2011)
  • Author(s): Kim DY, et al
  • Journal Title: J Immunol Volume: 186(7) Issue: 7 Pages: 4253-4262
  • DOI: 10.4049/jimmunol.0903794
  • Peer Reviewed
• [Presentation] Discovery of novel cell surface markers and two non-redundant pathways for the development of Perey' s patch M cells (2012)
  • Author(s): Shintaro Sato, Satoshi Kaneto, Naoko Shibata, Hideaki Okura, and Hiroshi Kiyono
  • Organizer: 第41回日本免疫学会総会・学術集会
  • Place of Presentation: 神戸
• [Presentation] Discovery of novel cell surface markers and two non-redundant pathways for the development of Perey’s patch M cells. (2012)
  • Author(s): Shintaro Sato
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸