Rehabilitation of cancer cells

• Project/Area Number: 23659206
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Juntendo University
• Principal Investigator: HINO Okio 順天堂大学, 医学部, 教授 (90127910)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 疾患モデル動物 / リプログラミング / iPS化 / Tsc変異体 / がん幹細胞 / 遺伝性がん

Research Abstract

Normal rat’s and Eker (Tsc2+/-) rat’s ESCs were established. Oct4, Sox2, Nanog were expressed in these ERCs. We injected ESCs under the kidney capsule of nude mice. Tsc2-/-teratomas were capable to differentiate into various cell types derived from three germ layers. However, they showed abnormal epithelial structures, resemble to RCC tissues in Eker rats. We suggest that tissue specific abnormal cellular differentiation caused by Tsc2-deficiency was recaptured by teratoma formation

Research Products

• [Journal Article] NP-1250, an ABCG2 inhibitor, induces apoptotic cell death in breast carcinoma MCF7/mitoxantrone-resistant cells via a caspase-independent pathway (2013)
  • Author(s): Ito M., Kajino K., Abe M., Fujimura T., Mineki R., Ikegami T., Ishikawa T. and Hino O
  • Journal Title: Oncology Reports Volume: 29 Pages: 1492-1500
• [Journal Article] TSC1 controls distribution of actin fibers through its effect on function of Rho family of small GTPases and regulates cell migration and polarity (2013)
  • Author(s): Osawa M., Kobayashi T., Okura H., Igarashi T., MizuguchiM. and Hino O
  • Journal Title: Plos One Volume: 8 Pages: 1-13
• [Journal Article] Up-regulation of dbpA mRNA in hepatocellular carcinoma associated with metabolic syndrome (2013)
  • Author(s): Obulhasim G., Yasen M., Kajino K., Mogushi K., Tanaka S., Mizushima H., Tanaka H., Arii S. and Hino O
  • Journal Title: Hepatol. Int Volume: 7 Pages: 215-225
• [Journal Article] Tuberin activates and controls the distribution of Rac1 via association with p62 and ubiquitin through the mTORC1 signaling pathway. (2013)
  • Author(s): Okura, H., Kobayashi, T., Koike, M., Ohsawa, M., Zhang, D., Arai, H., Uchiyama, Y., *Hino O
  • Journal Title: Int J Oncol Volume: 43 Issue: 2 Pages: 447-456
  • DOI: 10.3892/ijo.2013.1984
  • Peer Reviewed
• [Journal Article] NP-1250, an ABCG2 inhibitor, induces apoptotic cell death in breast carcinoma MCF7/mitoxantrone-resistant cells via a caspase-independent pathway. (2013)
  • Author(s): Ito M
  • Journal Title: Int. J. Oncology Volume: 29 Pages: 1492-1500
  • Peer Reviewed
• [Journal Article] TSC1 controls distribution of actin fibers through its effect on function of Rho family of small GTPases and regulates cell migration and polarity. (2013)
  • Author(s): Osawa M
  • Journal Title: Plos One Volume: 8
  • Peer Reviewed
• [Journal Article] Up-regulation of dbpA mRNA in hepatocellular carcinoma associated with metabolic syndrome. (2013)
  • Author(s): Obulhasim G
  • Journal Title: Hepatol. Int Volume: 7 Pages: 215-225
  • Peer Reviewed
• [Journal Article] Ikaros is a critical target during simultaneous exposure to X-rays and N-ethyl-N-nitrosourea in mouse T-cell lymphomagenesis. (2013)
  • Author(s): Hirano S
  • Journal Title: International J. Cancer Volume: in press
  • Peer Reviewed
• [Journal Article] DNA binding protein A exression and methylation status in hepatocellular carcinoma and the adjacent tissue (2012)
  • Author(s): Yasen M., Obulhasim G., Kajino K., Mogushi K., Mizushima H., Tanaka S., Tanaka H., Hino O. and Arii S
  • Journal Title: Int. J. Oncology Volume: 40 Pages: 789-797
• [Journal Article] Preventing of alveolar destruction and airspace enlargement in a mouse Model of pulmonary lymphangioleiomyomatosis (LAM) (2012)
  • Author(s): Goncharova E.A., Goncharov D.A., Fehrenbach M., Khavin I., Ducka B., Hino O., Colby T.V., Merrilees M.J., Haczku A., Albelada S.M. and Krymskaya V.
  • Journal Title: Science Translational Medicine Volume: 4 Pages: 1-10
• [Journal Article] Rapamycin reverses impaired social interaction in mouse models of tuberous sclerosis complex (2012)
  • Author(s): Sato A., Kasai S., Kobayashi T., Takamatsu Y., Hino O., Ikeda K. and Mizuguchi M
  • Journal Title: Nature Communications Volume: 2295 Pages: 1-9
• [Journal Article] DNA binding protein A exression and methylation status in hepatocellular carcinoma and the adjacent tissue. (2012)
  • Author(s): Yasen M
  • Journal Title: Int. J. Oncology Volume: 40 Pages: 789-797
  • Peer Reviewed
• [Journal Article] Preventing of alveolar destruction and airspace enlargement in a mouse Model of pulmonary lymphangioleiomyomatosis (LAM). (2012)
  • Author(s): Goncharova E.A.
  • Journal Title: Science Translational Medicine Volume: 4 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Rapamycin reverses impaired social interaction in mouse models of tuberous sclerosis complex. (2012)
  • Author(s): Sato A
  • Journal Title: Nature Communications Volume: 2295 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Autophagy-deficient mice develop multiple liver tumors (2011)
  • Author(s): Takamura A., Komatsu M., Hara T., Sakamoto A., Kishi C., Waguri S., Eishi Y., Hino O., Tanaka K. and Mizushima N
  • Journal Title: Gene & Development Volume: 25 Pages: 795-800
• [Journal Article] HCV core protein promotes heparin binding EGF-like growth factor expression and activates Akt (2011)
  • Author(s): Nakamura H., Aoki H., Hino O. and Moriyama M
  • Journal Title: Hepatology Research Volume: 4 Pages: 455-462
  • NAID: 10031144111
• [Journal Article] Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells (2011)
  • Author(s): Inami Y., Waguri S., Sakamoto A., Kouno T., Nakada K., Hino O., Watanabe S., Andoh J., Iwadate M., Yamamoto M., Lee M-S., Tanaka K. and Komatsu M
  • Journal Title: J. Cell Biology Volume: 193 Pages: 275-284
• [Journal Article] Deficiency of the Erc/mesothelin gene ameliorates renal carcinogenesis in Tsc2 knockout mice. (2011)
  • Author(s): Zhang D.
  • Journal Title: Cancer Science Volume: 102 Pages: 720-727
  • Peer Reviewed
• [Journal Article] Autophagy-deficient mice develop multiple liver tumors. (2011)
  • Author(s): Takamura A.
  • Journal Title: Gene & Development Volume: 25 Pages: 275-284
  • Peer Reviewed
• [Journal Article] Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells. (2011)
  • Author(s): Inami Y.
  • Journal Title: J. Cell Biology Volume: 193 Pages: 275-284
  • Peer Reviewed
• [Journal Article] Ikaros is a critical target during simultaneous exposure to X-rays and N-ethyl-N-nitrosourea in mouse T-cell lymphomagenesis
  • Author(s): Hirano S., Kakinuma S., Amasaki Y., Nishimura M., Imaoka T., Fujimono S., Hino O. and Shimada Y
  • Journal Title: International J. Cancer Volume: (in press)