| Project/Area Number |
23659208
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine (2012-2013)
The University of Tokyo (2011) |
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Principal Investigator |
KATO Kentaro 帯広畜産大学, 原虫病研究センター, 特任准教授 (30401178)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Koichiro 東京大学, 医科学研究所, 准教授 (50179991)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | マラリア原虫 / 幹細胞 / 赤血球 |
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| Research Abstract |
We confirmed the invasion of Plasmodium falciparum using erythrocyte like cells from human umbilical cord blood hematopoietic stem cell and mouse ES cells. The establishment of effective production system of mature erythrocyte from stem cell has been mainly proceeded. Before the treatment of ES cell and iPS cell, the technique that CD34 positive hematopoietic stem cell are isolated from umbilical cord blood and cultured have been mastered. Afterwards, we investigated the effective condition to lead enucleation with arrangement of class, concentration of cytokine, and culture period.
Moreover, to produce the differentiated erythrocyte mutant with specific protein deficient, we tried to establish the trancfection system of differentiated erythrocyte using lentivirus vecter.
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