Development of a direct transfection method in Plasmodium falciparum
| Project/Area Number |
23659210
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Mie University |
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Principal Investigator |
YUDA Masao 三重大学, 大学院・医学系研究科, 教授 (90293779)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 原虫 / マラリア / 遺伝子導入 |
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| Research Abstract |
At present, genetic manipulation of Plasmodium falciparum, the most important human malaria parasite is carried out with indirect transfection method that is of low efficiencies. In this study we attempted to develop a direct transfection method in this parasite andimprove the efficiencies. By establishing a method to purify a large amount of mature schizonts and using a P. falciparum artificial chromosome, we succeeded in introducing a foreign DNA into the parasites with approximately 1000-fold efficiency compared to that with indirect transfection method. The method developed in this study will improve genetic manipulation of this parasite and have a great impact on this research field.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Altered gene and protein expression in liver of the obese spontaneously hypertensive/NDmcr-cp rat2012
Author(s)
Chang, J., S. Oikawa, G. Ichihara, Y. Nanpei, Y. Hotta, Y. Yamada, S. Tada-Oikawa, H. Iwahashi, E. Kitagawa, I. Takeuchi, M. Yuda & S. Ichihara
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Journal Title
Nutrition & metabolism
Volume: 9
Pages: 87-87
Related Report
Peer Reviewed
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