| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
At present, genetic manipulation of Plasmodium falciparum, the most important human malaria parasite is carried out with indirect transfection method that is of low efficiencies. In this study we attempted to develop a direct transfection method in this parasite andimprove the efficiencies. By establishing a method to purify a large amount of mature schizonts and using a P. falciparum artificial chromosome, we succeeded in introducing a foreign DNA into the parasites with approximately 1000-fold efficiency compared to that with indirect transfection method. The method developed in this study will improve genetic manipulation of this parasite and have a great impact on this research field.
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