| Project/Area Number |
23659211
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Osaka University |
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Principal Investigator |
TANABE Kazuyuki 大阪大学, 微生物病研究所, 招へい教授 (40047410)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Makoto 群馬大学, 大学院・医学系, 講師 (50326849)
HONMA Hajime 大阪大学, 微生物病研究所, 特任研究員 (10617468)
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| Research Collaborator |
HORII Toshihiro 大阪大学, 微生物病研究所, 教授 (80142305)
MITA Toshihiro 東京女子医科大学, 医学部, 講師 (80318013)
NAKAMURA Shota 大阪大学, 微生物病研究所, 助教 (90432434)
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| Project Period (FY) |
2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | マラリア / ミューテーター / Plasmodium / ゲノム / 進化 / 突然変異 |
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| Research Abstract |
The present study was aimed to establish an experimental model system for predicting evolution of malaria parasites using a highly mutagenic Plasmodium berghei(mutator malaria parasite), which has defective proof reading activity of the parasite DNA polymeraseδ. Genome-wide sequence analysis of spontaneous mutations in the mutator parasite, after passages in mice over two years, revealed that mutation rate in the mutator was 75 to 100 times higher than that of control. Further, results of sulfadoxine-resistance tests suggest that the mutator system can be a novel innovative research tool, which enables us to predict drug resistance of malaria parasites.
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