Identification of Helicobacter pylori-derived ncRNAs that induce host response disruption

• Project/Area Number: 23659220
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: The University of Tokyo
• Principal Investigator: MIMURO Hitomi 東京大学, 医科学研究所, 准教授 (80396887)
• Co-Investigator(Kenkyū-buntansha): MARUYAMA Fumito 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (30423122)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細菌 / 感染症 / ヘリコバクターピロリ

Research Abstract

The aim of this study was to explore effector RNAs in Helicobacter pylori, which disrupt host responses. In silico analysis revealed that there is no effector RNAs possessing human microRNA-like conformation in H. pylori genome sequence. We found that the levels of several microRNAs were increased independent of the bacterial RNA transfer in H. pylori-infected epithelial cells. The effect of the identified microRNA increased in H. pylori-infected cells was estimated as the cell proliferation of host cells.

Research Products

• [Journal Article] A bacterial effector targets the TRAF6-NFκB pathway to modulate the acute inflammatory response to bacterial invasion of epithelial cells. (2012)
  • Author(s): Sanada T
  • Journal Title: Virulence Volume: 3 Issue: 6 Pages: 518-21
  • DOI: 10.4161/viru.21451
  • Peer Reviewed
• [Journal Article] Uptake of Shigella-containing pseudopodia by neighboring epithelial cells at tricellular junctions via non-canonical clathrin-dependent trafficking pathway. (2012)
  • Author(s): Fukumatsu M
  • Journal Title: Virulence Volume: 3 Issue: 6 Pages: 515-8
  • DOI: 10.4161/viru.21740
  • Peer Reviewed
• [Journal Article] Cell death and infection : a double-edged sword for host and pathogen survival (2011)
  • Author(s): Ashida H, Mimuro H, Ogawa M, Kobayashi T, Sanada T, Kim M, Sasakawa C
  • Journal Title: J Cell Biol Volume: 195 Issue: 6 Pages: 931-942
  • DOI: 10.1083/jcb.201108081
  • Peer Reviewed
• [Journal Article] Cell death and infection: a double-edged sword for host and pathogen survival. (2011)
  • Author(s): Ashida, H., Mimuro, H., Ogawa, M., Kobayashi, T., Sanada, T., Kim, M. and Sasakawa, C.
  • Journal Title: J Cell Biol Volume: 195 Pages: 931-942
  • Peer Reviewed
• [Presentation] Control of host cell responses by Helicobacter pylori infection (2012)
  • Author(s): Mimuro H, Kiga K, Sasakawa C.
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎新聞文化ホール , 長崎県
  • Year and Date: 2012-03-28
• [Presentation] Control of host cell responses by Helicobacter pylori infection (2012)
  • Author(s): Hitomi Mimuro, Kotaro Kiga, and Chihiro Sasakawa
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎新聞文化ホール
• [Presentation] Control of host cell responses by Helicobacter pylori infection (2011)
  • Author(s): Mimuro H, Kiga K, Sasakawa C.
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター, 北海道
  • Year and Date: 2011-09-08
• [Presentation] Control of host cell responses by Helicobacter pylori infection (2011)
  • Author(s): Hitomi Mimuro, Kotaro Kiga, and Chihiro Sasakawa
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress(招待講演)
  • Place of Presentation: 札幌コンベンションセンター
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/Mimuro_Lab/index.html