Development of a novel technique to monitor gene expression profiles of pathogenic bacteria during infection process
Project/Area Number |
23659222
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bacteriology (including Mycology)
|
Research Institution | Osaka University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | IVET / マイクロアレイ / 気管支敗血症菌 / 百日咳 / 遺伝子発現 / 百日咳菌 |
Research Abstract |
Analyses for gene expression of pathogens in response to the host environment provide clues to understanding the underlying events to establish the infectious diseases. In this study, we established a novel method named IVET-IP (in vivo expression tag immunoprecipitation system) to enable us to identify genes expressed in the host environment. By using this method, we determined genes expressed in Bordetella bronchiseptica in rats during infection process. As a result, we found that the in vivo expression pattern of bacterial virulence genes were quite different from that in vitro. In addition, at least 289 gene loci of Bordetella bronchiseptica were found to be expressed >2-fold in rats compared to in vitro.
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Report
(3 results)
Research Products
(32 results)
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[Journal Article] Defining the substrate specificity of Pasteurella multocida toxin-induced deamidation of α-subunits of heterotrimeric G proteins2013
Author(s)
Orth, J. H., Fester, I., Siegert, P., Weise, M., Lanner, U., Kamitani, S., Tachibana, T., Wilson, B. A., Schlosser, A., Horiguchi, Y., and Aktories,K
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Journal Title
FASEB J
Volume: 27
Issue: 2
Pages: 832-842
DOI
Related Report
Peer Reviewed
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[Journal Article] Crystal Structure of Clostridiumperfringensenterotoxin displays features of β-pore-forming toxins2011
Author(s)
Kitadokoro, K., Nishimura, K., Kamitani, S., Fukui-Miyazaki, A., Toshima, H., Abe, H., Kamata, Y., Sugita-Konishi, Y., Yamamoto, S., Karatani, H., and Horiguchi, Y
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Journal Title
J Biol Chem
Volume: 286
Issue: 22
Pages: 19549-19555
DOI
Related Report
Peer Reviewed
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[Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq2011
Author(s)
Kamitani, S., Ao, S., Toshima, H., Tachibana, T., Hashimoto, M., Kitadokoro, K., Fukui-Miyazaki, A., Abe, H., and Horiguchi, Y.
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Journal Title
FEBS J
Volume: 278
Issue: 15
Pages: 2702-2712
DOI
Related Report
Peer Reviewed
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