Development of a novel technique to monitor gene expression profiles of pathogenic bacteria during infection process

• Project/Area Number: 23659222
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: HORIGUCHI Yasuhiko 大阪大学, 微生物病研究所, 教授 (00183939)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: IVET / マイクロアレイ / 気管支敗血症菌 / 百日咳 / 遺伝子発現 / 百日咳菌

Research Abstract

Analyses for gene expression of pathogens in response to the host environment provide clues to understanding the underlying events to establish the infectious diseases. In this study, we established a novel method named IVET-IP (in vivo expression tag immunoprecipitation system) to enable us to identify genes expressed in the host environment. By using this method, we determined genes expressed in Bordetella bronchiseptica in rats during infection process. As a result, we found that the in vivo expression pattern of bacterial virulence genes were quite different from that in vitro. In addition, at least 289 gene loci of Bordetella bronchiseptica were found to be expressed >2-fold in rats compared to in vitro.

Research Products

• [Journal Article] Defining the substrate specificity of Pasteurella multocida toxin-induced deamidation of α-subunits of heterotrimeric G proteins (2013)
  • Author(s): Orth, J. H., Fester, I., Siegert, P., Weise, M., Lanner, U., Kamitani, S., Tachibana, T., Wilson, B. A., Schlosser, A., Horiguchi, Y., and Aktories,K
  • Journal Title: FASEB J Volume: 27 Issue: 2 Pages: 832-842
  • DOI: 10.1096/fj.12-213900
  • Peer Reviewed
• [Journal Article] Swine Atrophic Rhinitis caused by Pasteurella multocida toxin and Bordetella dermonecrotic toxin (2012)
  • Author(s): Y. Horiguchi
  • Journal Title: Current Topics in Microbiology and Immunology Volume: 361 Pages: 113-129
  • DOI: 10.1007/82_2012_206
  • ISBN: 9783642310164, 9783642310171
  • Peer Reviewed
• [Journal Article] Swine Atrophic Rhinitis Caused by Pasteurella Multocida Toxin and Bordetella Dermonecrotic Toxin. (2012)
  • Author(s): Horiguchi, Y.
  • Journal Title: Curr Top Microbiol Immunol Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Crystal Structure of Clostridiumperfringensenterotoxin displays features of β-pore-forming toxins (2011)
  • Author(s): Kitadokoro, K., Nishimura, K., Kamitani, S., Fukui-Miyazaki, A., Toshima, H., Abe, H., Kamata, Y., Sugita-Konishi, Y., Yamamoto, S., Karatani, H., and Horiguchi, Y
  • Journal Title: J Biol Chem Volume: 286 Issue: 22 Pages: 19549-19555
  • DOI: 10.1074/jbc.m111.228478
  • Peer Reviewed
• [Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq (2011)
  • Author(s): Kamitani, S., Ao, S., Toshima, H., Tachibana, T., Hashimoto, M., Kitadokoro, K., Fukui-Miyazaki, A., Abe, H., and Horiguchi, Y.
  • Journal Title: FEBS J Volume: 278 Issue: 15 Pages: 2702-2712
  • DOI: 10.1111/j.1742-4658.2011.08197.x
  • Peer Reviewed
• [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids (2011)
  • Author(s): Fukui-Miyazaki A, Ohnishi S, Kamitani S, Abe H, Horiguchi Y
  • Journal Title: Microbiol Immunol Volume: 55 Issue: 3 Pages: 154-159
  • DOI: 10.1111/j.1348-0421.2010.00300.x
  • NAID: 10031121454
  • Peer Reviewed
• [Presentation] Attempts to understand how the pathogenic bordetellae exert specific pathogenicity in specific hosts (2013)
  • Author(s): Horiguchi Y
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2013-03-29
• [Presentation] A putative virulence factor produced by Bordetella (2013)
  • Author(s): Nishikawa S, Abe H, Okada K, Kamitani S, Fukui A, Horiguchi Y
  • Organizer: 第86回日本細菌学会
  • Place of Presentation: 千葉市 幕張メッセ
  • Year and Date: 2013-03-18
• [Presentation] IVET-IP Analysis Monitoring Temporal Gene Expression Profiles of Bordetella bronchisepticain Rats (2013)
  • Author(s): Abe H, Kamitani S, Fukui A, Okada K, Horiguchi Y
  • Organizer: 第86回日本細菌学会
  • Place of Presentation: 千葉市 幕張メッセ
  • Year and Date: 2013-03-18
• [Presentation] IVET-IP Analysis Monitoring Temporal Gene Expression Profiles of Bordetella bronchiseptica in Rats (2013)
  • Author(s): H. Abe
  • Organizer: 第86回日本細菌学会
  • Place of Presentation: 横浜市幕張メッセ
• [Presentation] A putative virulence factor produced by Bordetella (2013)
  • Author(s): S. Nishikawa
  • Organizer: 第86回日本細菌学会
  • Place of Presentation: 横浜市幕張メッセ
• [Presentation] Attempts to understand how the pathogenic bordetellae exert specific pathogenicityi in specific hosts (2013)
  • Author(s): Y. Horiguchi
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学駒場キャンパス
  • Invited
• [Presentation] なぜ百日咳菌はヒトだけに感染して激しい咳発作を起こすのか?その基礎細菌学的アプローチ (2012)
  • Author(s): 堀口安彦
  • Organizer: 第39回地方衛生研究所全国協議会近畿支部細菌部会研究会
  • Place of Presentation: 大阪市 天王寺区役所
  • Year and Date: 2012-11-09
• [Presentation] A functional difference in adenylate cyclase toxins produced by Bordetella pertussisand Bordetella bronchiseptica (2012)
  • Author(s): Horiguchi Y
  • Organizer: IEIIS 2012 Homeostatic Inflammation Symposium
  • Place of Presentation: National Center of Sciences Building, Tokyo.
  • Year and Date: 2012-10-25
• [Presentation] ボルデテラ属細菌の感染特異性決定機構解析へのアプローチ. (2012)
  • Author(s): 堀口安彦
  • Organizer: 第79回日本細菌学会北海道支部学術集会
  • Place of Presentation: 北海道帯広市 とかちプラザ
  • Year and Date: 2012-08-28
• [Presentation] Different activities of adenylate cyclase toxins of Bordetella pertussisand B.bronchiseptica (2012)
  • Author(s): 戸嶋ひろ野, 福井理, 安倍裕順, 神谷重樹, 堀口安彦
  • Organizer: 日本細菌学会
  • Place of Presentation: 長崎市 長崎ブリックホール
  • Year and Date: 2012-03-27
• [Presentation] The molecular action of Clostridium perfringensenterotoxin,a pore-forming toxin (2012)
  • Author(s): Horiguchi Y
  • Organizer: 日本細菌学会
  • Place of Presentation: 長崎市 長崎ブリックホール.
  • Year and Date: 2012-03-27
• [Presentation] A functional difference in adenylate cyclase toxins produced by Bordetella pertussis and Bordetellabronchiseptica (2012)
  • Author(s): Y. Horiguchi
  • Organizer: IEIIS 2012 Homeostatic Inflammation Symposium
  • Place of Presentation: National Center of Sciences Building, Tokyo
  • Invited
• [Presentation] ボルデテラ属細菌の感染特異性決定機構解析へのアプローチ (2012)
  • Author(s): 堀口安彦
  • Organizer: 第79回日本細菌学会北海道支部学術集会
  • Place of Presentation: 北海道帯広市とかちプラザ
  • Invited
• [Presentation] 「なぜ百日咳菌はヒトだけに感染して激しい咳発作を起こすのか?」 その基礎細菌学的アプローチ (2012)
  • Author(s): 堀口安彦
  • Organizer: 第39回地方衛生研究所全国協議会近畿支部細菌部会研究会
  • Place of Presentation: 大阪市天王寺区役所
  • Invited
• [Presentation] Different Activities of Adenylate Cyclase Toxins of Bordetella Pertussis and B.Bronchiseptica. (2012)
  • Author(s): 戸嶋ひろ野, 福井理, 安倍裕順, 神谷重樹, 堀口安彦.
  • Organizer: 日本細菌学会
  • Place of Presentation: 長崎ブリックホール（長崎）
• [Presentation] The molecular action of Clostridium perfringens enterotoxin, a pore-forming toxin. (2012)
  • Author(s): Horiguchi Y
  • Organizer: 日本細菌学会
  • Place of Presentation: 長崎ブリックホール（長崎）
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順, 神谷重樹, 福井理, 戸嶋ひろ野, 堀口安彦
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市 パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順, 神谷重樹, 福井理, 戸嶋ひろ野, 堀口安彦
  • Organizer: 日本細菌学会関西支部総会
  • Place of Presentation: 大阪市 大阪府立大学中百舌鳥キャンパス
  • Year and Date: 2011-11-19
• [Presentation] The pore-forming mechanism of clostridium perfringens enterotoxin targeting claudins,components of the tight junction (2011)
  • Author(s): Horiguchi Y, Kitadokoro K, Nishimura K, Kamitani S, Abe H
  • Organizer: 日本生化学会
  • Place of Presentation: 京都市 京都国際会議場.
  • Year and Date: 2011-09-23
• [Presentation] The pore-forming mechanism of clostridium perfringens enterotoxin targeting claudins, components of the tight junction (2011)
  • Author(s): Horiguchi Y, Kitadokoro K, Nishimura K, Kamitani S, Abe H
  • Organizer: International Union of Microbiological Societies2011
  • Place of Presentation: 札幌市 札幌コンベンションセンター
  • Year and Date: 2011-09-07
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順, 神谷重樹, 福井理, 戸嶋ひろ野, 堀口安彦
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順, 神谷重樹, 福井理, 戸嶋ひろ野, 堀口安彦
  • Organizer: 日本細菌学会関西支部総会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス（大阪 堺）
• [Presentation] The Pore-Forming Mechanism of Clostridium perfringens enterotoxin targeting caludins, components of the tight junction. (2011)
  • Author(s): Horiguchi Y, Kitadokoro K, Nishimura K, Kamitani S, and Abe H
  • Organizer: 日本生化学会
  • Place of Presentation: 京都国際会議場（京都）
• [Presentation] The Pore-Forming Mechanism of Clostridium perfringens enterotoxin targeting caludins, components of the tight junction. (2011)
  • Author(s): Horiguchi Y, Kitadokoro K, Nishimura K, Kamitani S, and Abe H
  • Organizer: International Union of Microbiological Societies 2011
  • Place of Presentation: 札幌コンベンションセンター（札幌）
• [Book] Swine Atrophic Rhinitis Caused by Pasteurella multocidaToxin and BordetellaDermonecrotic Toxin
  • Author(s): Horiguchi Y
  • Publisher: Curr Top Microbiol Immunol(Springer)
• [Remarks]
  • URL: http://bactox1.biken.osaka-u.ac.jp/2011Web/Horiguchis_Lab_top.html