Investigation of the molecular targets for regulation of HIV-1 expression in latently infected cells

• Project/Area Number: 23659233
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Kagoshima University
• Principal Investigator: BABA Masanori 鹿児島大学, 大学院医歯学総合研究科, 教授 (70181039)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Mika 鹿児島大学, 大学院医歯学総合研究科, 講師 (90336347)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: HIV / 潜伏感染 / 遺伝子発現 / 宿主因子 / 活性化 / HIV-1 / 慢性潜伏感染 / Galectin-3 / TNF-α

Research Abstract

Microarray analysis revealed that 15 cellular factor genes were significantly upregulated along with HIV-1 expression in latently infected cells stimulated with TNF-α. Among the factors, Galectin-3 was found to promote HIV-1 expression through NF-κB activation in the cells.

Research Products

• [Journal Article] Sustained inhibition of HIV-1 replication by conditional expression of the E. coli-derived endoribonuclease MazF in CD4^+ T cells (2013)
  • Author(s): Okamoto M, Chono H, Kawano Y, Saito N, Tsuda H, Inoue K, Kato I, Mineno J, Baba M
  • Journal Title: Hum. Gene. Ther. Methods Volume: 24 Issue: 2 Pages: 94-103
  • DOI: 10.1089/hgtb.2012.131
  • Peer Reviewed
• [Journal Article] Identification of novel inhibitors of human immunodeficiency virus type 1 replication by in silico screening targeting cyclinT1/Tat interaction (2013)
  • Author(s): Takayuki Hamasaki
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 57 Issue: 3 Pages: 1323-1331
  • DOI: 10.1128/aac.01711-12
  • Peer Reviewed
• [Journal Article] 抗ウイルス薬の歴史と分類 (2013)
  • Author(s): 馬場昌範
  • Journal Title: 臨床と微生物 Volume: 40 Pages: 3-7
• [Journal Article] Anti-human immunodeficiency virus type 1 activity of novel 6-substituted 1-benzyl-3-(3,5-dimethylbenzyl)uracil derivatives (2012)
  • Author(s): Paula Ordnnez
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 56 Issue: 5 Pages: 2639-2646
  • DOI: 10.1128/aac.06307-11
  • Peer Reviewed
• [Journal Article] 抗ウイルス薬研究の歴史と進歩 (2012)
  • Author(s): 馬場昌範
  • Journal Title: 日本臨床 Volume: 70 Pages: 553-557
• [Presentation] Tat依存性のHIV-1産生を抑制する新規低分子化合物の同定 (2012)
  • Author(s): 濱崎隆之,岡本実佳,馬場昌範
  • Organizer: 第26回日本エイズ学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2012-11-25
• [Presentation] 核酸系逆転写酵素阻害薬フェスティナビル (2011)
  • Author(s): 馬場昌範
  • Organizer: 第25回日本エイズ学会学術集会シンポジウム「次世代の新薬の最新情報」
  • Place of Presentation: 東京
  • Year and Date: 2011-12-02
• [Presentation] Differential expression of host cellular factors upon HIV-1 reactivation (2011)
  • Author(s): Ordonez-Suarez P, Hamasaki T, Baba M, Okamoto M
  • Organizer: 24th International Conference on Antiviral Research
  • Place of Presentation: Sofia, Bulgaria
  • Year and Date: 2011-05-09
• [Presentation] Long-term inhibition of HIV-1 replication in CD4^+ T cells transduced with a retroviral vector conditionally expressing the Escherichia coli endoribonuclease MazF (2011)
  • Author(s): Okamoto M, Chono H, Tsuda H, Inoue K, Mineno J, Baba M
  • Organizer: 24th International Conference on Antiviral Research
  • Place of Presentation: Sofia, Bulgaria
  • Year and Date: 2011-05-09
• [Presentation] In silico screening of compounds targeting human cyclin T1 and in vitro evaluation of their anti-HIV-1 activity (2011)
  • Author(s): Hamasaki T, Okamoto M, Baba M
  • Organizer: 24th International Conference on Antiviral Research
  • Place of Presentation: Sofia, Bulgaria
  • Year and Date: 2011-05-09
• [Presentation] HIV の活性化における宿主細胞因子の異なった発現 (2011)
  • Author(s): オルドネス・スアレス パウラ，濱崎隆之，馬場昌範，岡本実佳
  • Organizer: 第２４回国際抗ウイルス会議
  • Place of Presentation: ソフィア（ブルガリア）
• [Presentation] 薬剤耐性ウイルスと抗ウイルス薬の開発戦略 (2011)
  • Author(s): 馬場昌範
  • Organizer: 第２１回感染研シンポジウム「病原体と薬剤耐性」(招待講演)
  • Place of Presentation: 東京
• [Book] 抗ウイルス薬の開発.柳 雄介,堤 裕幸(編集)「新編 ウイルスの今日的意味」 (2012)
  • Author(s): 馬場昌範
  • Publisher: 医薬ジャーナル社
• [Book] ウイルス病の治療.平松啓一(監修),中込 治,神谷 茂(編集)標準微生物学(第11版) (2012)
  • Author(s): 馬場昌範(分担)
  • Publisher: 医学書院
• [Book] ウイルス感染症の治療.東 臣伸,小熊恵二,堀田 博(編集)シンプル微生物学(第5版) (2011)
  • Author(s): 馬場昌範(分担)
  • Publisher: 南江堂
• [Remarks]
  • URL: http://www.kufm.kagoshima-u.ac.jp/~ccvd/
• [Patent(Industrial Property Rights)] 抗ウイルスヌクレオシド類似体およびウイルス感染特にHIV感染の処置方法 (2012)
  • Inventor(s): 田中博道,馬場昌範,チェン ユン-チ
  • Industrial Property Rights Holder: エール ユニヴァーシティ,田中博道,馬場昌範
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2012-07-18
• [Patent(Industrial Property Rights)] 抗HCV薬 (2011)
  • Inventor(s): 馬場昌範,モハメド タハ アーメッド サリモ,下茂徹朗,武次祐樹
  • Industrial Property Rights Holder: 国立大学法人鹿児島大学
  • Industrial Property Rights Type: 特許
  • Patent Publication Number: 2012-232955
  • Filing Date: 2011-05-09