Role of brain-infiltrating T lymphocytes in the pathogenesis of West Nile virus infection

• Project/Area Number: 23659237
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: KURANE Ichiro 国立感染症研究所, 副所長 (90278656)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Ryuji 独立行政法人国立病院機構相模原病院臨床研究センター, 室長 (70373470)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ウイルス / 感染症 / 微生物 / 脳神経 / 免疫学 / 脳・神経

Research Abstract

We characterized brain-infiltrating T lymphocytes in mice infected with West Nile virus (WNV). The expression of CD3, CD8, CD69 positively correlated with viral RNA levels. The results suggest that activated Th1-like cytotoxic CD8(+) T cells are expanded in the brains in response to WNV infection. T cell receptor analysis demonstrated that WNV-specific CD3(+)CD8(+) T cells expanding in the infected brains are highly oligoclonal. Further, the infiltrating CD8(+) T cells were WNV-specific, but not cross-reactive to Japanese encephalitis virus.

Research Products

• [Journal Article] High clonality of virus-specific T lymphocytes defined by TCR usage in the brains of mice infected with West Nile virus. (2011)
  • Author(s): Kitaura, K., Fujii, Y., Hayasaka, D., Matsutani, T., Shirai, K., Nagata, N., Lim, C.K., Suzuki, S., Takasak, T., Suzuki, R. and Kurane, I.
  • Journal Title: Journal of Immunology Volume: 187(8) Pages: 3919-3930
• [Journal Article] High Clonality of Virus-Specific T Lymphocytes Defined by TCR Usage in the Brains of mice infected with west nile virus (2011)
  • Author(s): Kazutaka Kitaura、Yoshiki Fujii、Daisuke Hayasake、Takaji Matsutani、Kenji Shirai、Noriyo Nagata、Chang-Kweng Lim、Satsuki Suzuki、Tomohiko Takasaki、Ryuji Suzuki、Ichiro Kurane
  • Journal Title: Journal of Immunology Volume: 187(8) Issue: 8 Pages: 3919-3930
  • DOI: 10.4049/jimmunol.1100442
  • Peer Reviewed
• [Presentation] 日本脳炎ウイルス感染マウスにおける脳炎発症にかかわる脳内浸潤T細胞の解析 (2012)
  • Author(s): 白井顕治、北浦一孝、早坂大輔、高崎智彦、鈴木隆二、倉根一郎
  • Organizer: 第60回日本ウイルス学会学術集会
• [Presentation] 日本脳炎ウイルス感染においてTNFαは免疫応答を調節し重症化の抑制に働く (2012)
  • Author(s): 早坂大輔、青木康太郎、北浦一孝、白井顕治、Dash Sima Simanti、永田典代、高松由基、鈴木隆二、森田公一
  • Organizer: 第60回日本ウイルス学会学術集会
• [Presentation] 日本脳炎ウイルス感染マウスにおける脳炎発症にかかわる脳内浸潤Ｔ細胞の解析
  • Author(s): 倉根一郎、白井顕治、北浦一孝、早坂大輔、高崎智彦、鈴木隆二
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪国際会議場