| Project/Area Number |
23659283
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied pharmacology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Yoshinobu 京都大学, 大学院・薬学研究科, 教授 (30171432)
TAKAHASHI Yuki 京都大学, 大学院・薬学研究科, 教授 (00547870)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ドラッグデリバリー / 細胞治療 / 体内分布 / イメージング / 細胞個性 / 細胞スフェロイド / PEG修飾 |
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| Research Abstract |
To realized cell therapy, we tried to develop “targeted cell therapeutic systems based on cell characteristics”. We examined the use of multicellular spheroids as a method to maximize the cell survival and functions after in vivo application. Spheroids with controlled size were obtained by using polydimethylsiloxane-based micro-pockets, but cell adhesion to the micro-pocket surface prevented some types of cells from spheroid formation. Coating the micro-pockets with poly(N-isopropylacrylamide) solved this problem, and multicellular spheroids we re successfully prepared using all the cell types used. Then, therapeutic potential of multicellular spheroids was examined in diabetic model mice using insulin-producing NIT -1 cells. NIT-1 spheroids showed prolonged survival of the mice and high activity to reduce blood glucose level compared with NIT -1 cell suspension.
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