| Project/Area Number |
23659284
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied pharmacology
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| Research Institution | Kyoto University |
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Principal Investigator |
HASHIDA Mitsuru 京都大学, 大学院・薬学研究科, 教授 (20135594)
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| Co-Investigator(Renkei-kenkyūsha) |
HIGUCHI Yuriko 京都大学, 大学院・薬学研究科, 助教 (40402797)
HASHIDA Yasuhiko 京都大学, 物質-細胞統合システム拠点, 特定研究員 (30512462)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ドラッグデリバリー / 細胞治療 / 遺伝子発現長期化 / トランスポゾン / 細胞・遺伝子治療 / 遺伝子・細胞治療 / 長期発現 |
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| Research Abstract |
Transplantation of cells expressing therapeutic protein is a promising way to supply such proteins for long time. Such gene-cell therapy would be a novel treatment for chronic or congenital disease. In this study, we could established primary mesenchymal stem cells with long-term expression of target protein by transformation using transposon. These cells kept expressing the target protein after differentiation. Moreover, we could show the possible method for controlling insertion site in transformation using DNA-binding protein.
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